Washed platelets (1.5 × 105 platelets/µl) were maintained in Tyrode’s buffer (unactivated) or treated with 30 µM ADP (activated). Both unactivated and activated platelets were incubated for 6 min at room temperature with either control liposomes (Lipo-C, 50 µg/ml) or sulfatide-containing liposomes (Lipo-S, 50 µg/ml) in the absence and presence of 50 µM of one of the following peptides: Dab2 SBP, Dab2 SBP R42A, or Dab2 SBP R42K. Platelets were fixed with 1% formalin in phosphate buffered saline and incubated with phycoerythrin-labelled anti-CD62P (P-selectin; Biolegend) antibody following manufacturer’s instructions. Antibody-bound platelets were quantified using a FacsAria flow cytometer.
Anti cd62p p selectin
Manufactured by BioLegend
Anti-CD62P (P-selectin) is a lab equipment product that detects the expression of the CD62P cell surface antigen, also known as P-selectin. P-selectin is a cell adhesion molecule involved in the initial recruitment of leukocytes to sites of inflammation. This product can be used to measure P-selectin expression on activated platelets and endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti cd62p p selectin
1
Platelet P-selectin Expression Modulation
2
Sulfatide-mediated Platelet Activation Regulation
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Washed platelets (1.5 x 10 5 platelets/µl) were maintained in Tyrode's buffer (unactivated) or treated with 30 µM ADP (activated). Both unactivated and activated platelets were incubated for 6 min at room temperature with either control liposomes (Lipo-C, 50 µg/ml) or sulfatide-containing liposomes (Lipo-S, 50 µg/ml) in the absence and presence of 50 µM of one of the following peptides: Dab2 SBP, Dab2 SBP R42A, or Dab2 SBP R42K. Platelets were fixed with 1% formalin in phosphate buffered saline and incubated with phycoerythrin-labelled anti-CD62P (P-selectin; Biolegend) antibody following manufacturer's instructions. Antibody-bound platelets were quantified using a FacsAria flow cytometer.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 8, 2020. ; https://doi.org/10.1101/2020.05.06.081299 doi: bioRxiv preprint
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 8, 2020. ; https://doi.org/10.1101/2020.05.06.081299 doi: bioRxiv preprint
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