IL-1β and IL-18 levels in rat kidney tissues were quantified using ELISA kits (IL-1β: E-EL-R0012c; IL-18: E-EL-R0567c, Elabscience, Wuhan, China). Tissues were collected, weighed, minced, and lysed with RIPA buffer containing protease and phosphatase inhibitors. After homogenization, the lysates were centrifuged at 10,000 rpm for 15 minutes at 4°C to obtain the protein supernatants. The protein concentrations were then determined using a BCA Protein Assay Kit. Samples were then processed as per the ELISA kit instructions and analyzed with a microplate reader to determine IL-1β and IL-18 protein levels.
E el r0567c
Manufactured by Elabscience
Sourced in China
E-EL-R0567c is a laboratory equipment product from Elabscience. It serves as a Protein Purification Column.
Automatically generated - may contain errors
Lab products found in correlation
E el r0012c, by Elabscience (7 mentions)
E el r0015c, by Elabscience (3 mentions)
E el r0019c, by Elabscience (2 mentions)
Synergr lx, by Agilent Technologies (1 mentions)
Hemin, by Merck Group (1 mentions)
Corm 2, by Merck Group (1 mentions)
Multiskan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Ab1872, by Abcam (1 mentions)
Ab219800, by Abcam (1 mentions)
Znppix, by Merck Group (1 mentions)
Sc 374615, by Santa Cruz Biotechnology (1 mentions)
E el r0505c, by Elabscience (1 mentions)
E el r0016c, by Elabscience (1 mentions)
E el 0162c, by Elabscience (1 mentions)
E el r2856c, by Elabscience (1 mentions)
Csb e04640r, by Cusabio (1 mentions)
Csb e11987r, by Cusabio (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Csb e06869r, by Cusabio (1 mentions)
Csb e12654r, by Cusabio (1 mentions)
8 protocols using e el r0567c
1
Quantifying IL-1β and IL-18 in Rat Kidneys
2
Cytokine Profiling in Serum
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The levels of interleukin (IL)-6 (E-EL-R0015c; Elabscience, China), IL-1β (E-EL-R0012c; Elabscience), and IL-18 (E-EL-R0567c; Elabscience) in the serum were determined using ELISA kits and the 96-well plates placed into a multimode reader (SYNERGR LX; BioTEK).
3
Hemin and CORM-2 Inflammasome Modulation
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Hemin (no. 51280), zinc protoporphyrin IX (ZnPPIX) (no.282820), Carbon monoxide (CO)-releasing molecule 2 (CORM-2) (no.288144), and dextran 4000 Da (FD-4) (46944) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dimethylsulfoxide (DMSO) (D3870), phosphate buffer saline (PBS) (P1020), and 4% paraformaldehyde (P1110) were obtained from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). CORM-2 was dissolved in 0.5% of DMSO at 37°C in a sterile incubator for 24 h, and the resulting inactive CORM-2 (iCORM-2) was used as a negative control. Antibodies used were to caspase-1 (ab1872; Abcam, Shanghai, China), gasdermin D (GSDMD) (ab219800; Abcam, Shanghai, China), and caspase-11 p20 (sc-374615; Santa Cruz Biotechnology, Texas, USA). Enzyme-linked immunosorbent assay (ELISA) kits included rat tumor necrosis factor-α (TNF-α) (E-EL-R0019c), rat interleukin-1β (IL-1β) (E-EL-R0012c), rat IL-18 (E-EL-R0567c), and rat high mobility group box protein 1 (HMGB1) (E-EL-R0505c) (Elabscience Biotechnology Co., Ltd., Wuhan, China). The ELISA results were analyzed using a Multiskan Spectrum spectrophotometer (Thermofisher Scientific, Waltham, MA, USA).
4
Cytokine Profiling in Hippocampus
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After modeling (9 days after surgery), the whole blood and hippocampus tissues were harvested. After 2 h of standing, the whole blood was centrifuged for 3 min at 9,000 rpm. The serum was then collected to analyze the expression of cytokines. The hippocampus tissues were homogenized, and the levels of IL-1β (Elabscience, E-EL-R0012c), IL-6 (Elabscience, E-EL-R0015c), IL-12 (Elabscience, E-EL-R0064c), tumor necrosis factor (TNF)-α (Elabscience, E-EL-R2856c), IL-18 (Elabscience, E-EL-R0567c), colony-stimulating factor (CSF)-1 (Elabscience, E-EL-R0601c), IL-4 (Elabscience, E-EL-R0014c), IL-10 (Elabscience, E-EL-R0016c), transforming growth factor (TGF)-β (Elabscience, E-EL-0162c), and IL-13 (Elabscience, E-EL-R0563c) were determined according to the manufacturers’ instructions.
5
Quantifying IL-18 and IL-1β in MCAO Rats
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Rats were sacrificed 7 days after MCAO, and amounts of IL-18 and IL-1β in peri-infarct cortical tissues were measured with specific ELISA kits (E-EL-R0567c and E-EL-R0012c, respectively; Elabscience, China) as directed by the manufacturer. The results are shown as pictograms per milligram (pg/mg).
6
Cytokine and Estrogen Receptor Quantification
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ELISA kits of TNF‐α (CSB‐E11987r, CUSABIO, Wuhan, China), IL‐1β (E‐EL‐R0012c, Elabscience, Wuhan, China), IL‐18 (E‐EL‐R0567c, Elabscience), IL‐6 (CSB‐E04640r, CUSABIO) and ESR1 (CSB‐E06848r, CUSABIO) were employed to detect TNF‐α, IL‐1β, IL‐18 and IL‐6 levels in the medium of H92c cells and ESR1 levels in H92c cells according the manufacture's instructions.
7
Quantifying Brain Cytokine Levels
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The IL-1β and IL-18 levels in the brain tissues of each group were measured using the ELISA kits for IL-1β (E-EL-R0012c; Elabscience, Wuhan, China) and IL-18 (E-EL-R0567c; Elabscience) respectively according to the manufacturer’s instructions, and the protein concentration in the sample was detected using a BCA Protein Assay kit (Beyotime). Finally, the levels of IL-1β and IL-18 were normalized to the protein content in the corresponding sample.
8
Comprehensive Serum Biomarker Profiling
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Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the serum concentrations of HSP70 (DYC1663E, Minneapolis, MN, USA), follicle stimulating hormone (FSH)(CSB-E06869r, Cusabio Biotech CO.,Ltd., Wuhan, China), luteinizing hormone (LH) (CSB-E12654r, Cusabio Biotech CO.,Ltd., Wuhan, China), estradiol (E2) (CSB-E05110r, Cusabio Biotech CO.,Ltd., Wuhan, China), progesterone (P) (CSB-E07282r, Cusabio Biotech CO.,Ltd., Wuhan, China), testosterone (T)(E-EL-0072c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), insulin (CSB-E05070r, Elabscience Biotechnology Co.,Ltd., Wuhan, China), C-reactive protein (CRP) (E-EL-R0022c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), interleukin (IL)-6(E-EL-R0015c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), IL-18(E-EL-R0567c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), tumor necrosis factor (TNF)-α. (E-EL-R0019c, Elabscience Biotechnology Co.,Ltd., Wuhan, China). There were standard curves used to calculate for all these kits. The coefficients of variation within and between plates were less than 10%.
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