Kaleidagraph ver 3

Kaleidagraph ver. 3.51 (Synergy Software, Reading, PA) was applied to fit survival data of irradiated cells into LQ model. Mean values between two groups was compared using Student’s t test. All statistical analyses were undertaken using SPSS ver. 18.0 (SPSS Inc., Chicago, IL).

Kaleidagraph ver. 3.51 (Synergy Software, Reading, PA) was used to fit the survival data of irradiated cells into a linear quadratic model. Chi-square test was used for statistical analysis of in vitro experiments. Dose enhancement factor (DEF), defined as ratio of dose with radiation alone over radiation with drug for the same biologic effect was calculated. SAS program ver. 9.1 (SAS Institute, Cary, NC) was used for statistical analysis of in vivo experiments. ANOVA was used to determine the effect of drug and radiation for in vivo experiments. Fisher's exact test for immunohistochemistry analysis was used for comparison of differences between groups. Relative growth delay was defined as difference in growth delay between two groups. In the current study, difference of growth delay between IR and IR+VA group was calculated to test the role of VA in addition to radiation. In addition, enhancement factor for growth delay, defined as ratio of days required for the tumor to grow to a certain volume for IR group over IR+VA group was calculated.
Probability values less than 0.05 were considered statistically significant and less than 0.1 were considered of borderline significance.