Catechol-conjugated chitosan (CS-C) was synthesized using chitosan (Mw 100 kDa, 80% deacetylated; Sigma-Aldrich) and 3,4-dihydroxybenzaldehyde (3,4-DHB; Sigma-Aldrich) following previously published method by Clifford et al.26 The catechol was conjugated to the amine groups of chitosan by Schiff base reaction. Methanol was used to dissolve 3,4-DHB, NaBH4 (Sigma-Aldrich) as a reductant was used to reduce the C=N to C-N, and the degree of catechol substitution on the backbone of chitosan was determined by proton nuclear magnetic resonance. Stock solutions of CS-C (2 mg/mL, pH 6.5) were prepared for further use.
3 4 dhb
Manufactured by Merck Group
Sourced in United States
3,4-DHB is a chemical compound commonly used as a laboratory reagent. It is a dihydroxybenzoic acid derivative with the chemical formula C7H6O3. This compound is frequently utilized in various analytical and research applications within the scientific community.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
β glucuronidase, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Nabh4, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
2 protocols using 3 4 dhb
1
Catechol-Functionalized Chitosan Synthesis
2
Analytical Standards and Chemicals for LC-MS
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Analytical standards of MeP, EtP, n-PrP, n-BuP, 4-HB, and 3,4-DHB were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chlorzoxazone was purchased from National Institutes for Food and Drug Control (NIFDC, Beijing, China). β-glucuronidase (100,000 U/mL) was supplied by Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, methanol, ethyl acetate, ammonium acetate, and formic acid were supplied by Thermo Fisher Scientific (Houston, TX, USA). All the chemicals were of LC-MS or HPLC grade. Ultra-pure water was obtained from the Milli-Q system (Millipore, Bedford, MA, USA).
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