All chemicals were purchased from Sigma-Aldrich Australia (Castle Hill, NSW, Australia), Sigma-Aldrich USA (St Louis, MO, USA), or Merck Chemicals (Kilsyth, Victoria, Australia) with the exception of isopropyl-β-D-thiogalactopyranoside (IPTG) and streptomycin (Life Technologies, Victoria, Australia), tetrodotoxin (Alomone Labs, Israel), and HPLC-grade acetonitrile (RCI Labscan, Bangkok, Thailand). 13C6-glucose and 15NH4Cl were from Sigma-Aldrich Australia. Recombinant His6-TEV protease (EC 3.4.22.44) was produced in-house using a published protocol62 (link).
Isopropyl β d thiogalactopyranoside iptg and streptomycin
Manufactured by Thermo Fisher Scientific
Sourced in Australia
Isopropyl-β-D-thiogalactopyranoside (IPTG) is a chemical compound used as an inducer in bacterial expression systems. IPTG triggers the expression of genes under the control of the lac operon. Streptomycin is an antibiotic used for the selection of bacterial cells that have been transformed with a plasmid containing a streptomycin resistance gene.
Automatically generated - may contain errors
2 protocols using isopropyl β d thiogalactopyranoside iptg and streptomycin
1
Reagent Procurement for Biological Research
2
Reagent Procurement for Biological Research
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