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Actin aan01 a

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Actin (AAN01-A) is a purified protein from Cytoskeleton, Inc. It is a key structural component of the cytoskeleton in eukaryotic cells and is involved in various cellular processes such as cell motility, cell division, and intracellular transport.

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Lab products found in correlation

Ripk3, by Cell Signaling Technology (3 mentions) Alexa fluor 680 goat anti mouse, by Thermo Fisher Scientific (3 mentions) Goat anti mouse hrp, by Jackson ImmunoResearch (3 mentions) Goat anti rabbit hrp, by Jackson ImmunoResearch (3 mentions) Erk1 2, by Merck Group (2 mentions) Phospho p70 s6 kinase, by Cell Signaling Technology (2 mentions) P70 s6 kinase, by Santa Cruz Biotechnology (2 mentions) Ha 7 hrp, by Merck Group (2 mentions) Phospho stat5a b, by Merck Group (2 mentions) Hsp90, by BD (2 mentions) Phospho mek1 2, by Cell Signaling Technology (2 mentions) Stat5, by BD (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Mek1 2, by Cell Signaling Technology (2 mentions) Ir dye 800 donkey anti rabbit, by Rockland Immunochemicals (1 mentions) Ripk1, by BD (1 mentions)

Spelling variants of this product

AAN01 (4 citations)

3 protocols using actin aan01 a

1

Antibody Validation for Signaling Pathways

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Antibodies used were HA (SC-805, Santa Cruz, Dallas, TX), HA-7-HRP (H6533, Sigma-Aldrich), MEK1/2 (#9126, Cell Signaling, Danvers, MA), phospho-MEK1/2 (#2338, Cell Signaling), ERK1/2 (M5670, Sigma-Aldrich), phospho- ERK1/2 (#4370, Cell Signaling), STAT5 (610191, BD Biosciences, Franklin Lakes, NJ), phospho-STAT5A/B (05–886R, Merck Millipore), phospho-p70 S6 kinase (#9234, Cell Signaling), p70 S6 kinase (SC-230, Santa Cruz), RIPK3 (#12107, Cell Signaling), HSP90 (610418, BD Transduction Laboratories), actin (AAN01-A, Cytoskeleton, Denver, CO), and tubulin (ab7291, Abcam, Cambridge, UK). The secondary antibodies used were goat anti-mouse HRP (115–035-003, Jackson ImmunoResearch, West Grove, PA), goat anti-rabbit HRP (111–035-003, Jackson ImmunoResearch), and Alexa Fluor 680 goat anti-mouse (A-21057, Molecular probes, Grand Island, NY).
Bigenzahn J.W., Fauster A., Rebsamen M., Kandasamy R.K., Scorzoni S., Vladimer G.I., Müller A.C., Gstaiger M., Zuber J., Bennett K.L, & Superti-Furga G. (2015). An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client. Molecular & Cellular Proteomics : MCP, 15(3), 1139-1150.
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2

Antibodies Used in Cell Signaling

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Antibodies used were HA (SC-805, Santa Cruz, Dallas, TX, USA), HA-7-HRP (H6533, Sigma-Aldrich), MEK1/2 (#9126, Cell Signaling, Danvers, MA, USA), phospho-MEK1/2 (#2338, Cell Signaling), ERK1/2 (M5670, Sigma-Aldrich), phospho- ERK1/2 (#4370, Cell Signaling), STAT5 (610191, BD Biosciences, Franklin Lakes, NJ, USA), phospho-STAT5A/B (05-886R, Merck Millipore), phospho-p70 S6 kinase (#9234, Cell Signaling), p70 S6 kinase (SC-230, Santa Cruz), RIPK3 (12107, Cell Signaling), HSP90 (610418, BD Transduction Laboratories), actin (AAN01-A, Cytoskeleton, Denver, CO, USA), and tubulin (ab7291, Abcam, Cambridge, UK). The secondary antibodies used were goat anti-mouse HRP (115-035-003, Jackson ImmunoResearch, West Grove, PA, USA), goat anti-rabbit HRP (111-035-003, Jackson ImmunoResearch), and Alexa Fluor 680 goat anti-mouse (A-21057, Molecular probes, Grand Island, NY, USA).
Bigenzahn J.W., Fauster A., Rebsamen M., Kandasamy R.K., Scorzoni S., Vladimer G.I., Müller A.C., Gstaiger M., Zuber J., Bennett K.L, & Superti-Furga G. (2015). An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client. Molecular & Cellular Proteomics : MCP, 15(3), 1139-1150.
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3

Immunoblotting for Cell Signaling Proteins

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Antibodies used were p-IkBa (Ser32/36) (9246S, Cell Signaling, Danvers, MA, USA), actin (AAN01-A, Cytoskeleton, Denver, CO, USA), IkBa (SC-371, Santa Cruz, Dallas, TX, USA), tubulin (ab7291 Abcam), phospho-MLKL (Ser385) (ab187091, Abcam), RIPK1 (610458, BD Biosciences, Franklin Lakes, NJ, USA), RIPK3 (12107, Cell Signaling), HA (SC-805, Santa Cruz) and HA-7 (H6533, Sigma-Aldrich). The secondary antibodies used were goat anti-mouse HRP (115-035-003, Jackson ImmunoResearch, West Grove, PA, USA), goat anti-rabbit HRP (111-035-003, Jackson ImmunoResearch), Alexa Fluor 680 goat anti-mouse (A-21057, Molecular probes, Grand Island, NY, USA) and IRDye 800 donkey anti-rabbit (611-732-127, Rockland, Limerick, PA, USA).
Fauster A., Rebsamen M., Huber K.V., Bigenzahn J.W., Stukalov A., Lardeau C.H., Scorzoni S., Bruckner M., Gridling M., Parapatics K., Colinge J., Bennett K.L., Kubicek S., Krautwald S., Linkermann A, & Superti-Furga G. (2015). A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis. Cell Death & Disease, 6(5), e1767-.
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