Cytoset elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in Belgium

The CytoSet ELISA kit is a laboratory equipment product designed for the detection and quantification of specific analytes in a sample. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of the target analyte.

Automatically generated - may contain errors

Lab products found in correlation

Luminex 200, by Bio-Rad (2 mentions) Human inflammatory 5 plex panel, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ELISAs (35 citations) CytoSet (10 citations) CytoSet kits (7 citations) IL-8 cytoset ELISA kit (2 citations)

4 protocols using cytoset elisa kit

Cytokine Profiling of Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture supernatants were harvested after DC stimulation at indicated time points and frozen at -20°C until further analysis. TNFα was routinely measured by ELISA using the CytoSet ELISA kit (Biosource) according to the manufacturer’s protocol. For the multiplex bead immunoassay the Human Inflammatory 5-plex panel (Invitrogen, USA) was supplemented with a single-plex kit for IL-12 p70 (Invitrogen, USA). The assay was performed according to Invitrogen’s user manual ‘Human Inflammatory 5-plex panel’. Samples were measured by Luminex 200 (Bio-Rad, USA) and data analysis was performed using Bio-plex Manager 6.0 software. The TGF-β1 ELISA (Promega, G7591) was done according to the manufacturer’s protocol.

Klaver E.J., Kuijk L.M., Lindhorst T.K., Cummings R.D, & van Die I. (2015). Schistosoma mansoni Soluble Egg Antigens Induce Expression of the Negative Regulators SOCS1 and SHP1 in Human Dendritic Cells via Interaction with the Mannose Receptor. PLoS ONE, 10(4), e0124089.

+ Open protocol

+ Expand

Cytokine Profiling of Monocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The production of pro- and anti-inflammatory mediators was assessed by enzyme-linked immunosorbent assay (ELISA) in cell-free supernatants of vehicle- or SMI-treated CD40-stimulated monocytes using commercial kits for human IL-10, IL-6, and TNF-α CytoSet ELISA kit (Biosource, Nivelles, Belgium) according to the manufacturer’s protocol. The samples were measured using a Luminex 200 (Bio-Rad, Hercules, CA, USA).

Aarts S.A., Seijkens T.T., Kusters P.J., van der Pol S.M., Zarzycka B., Heijnen P.D., Beckers L., den Toom M., Gijbels M.J., Boon L., Weber C., de Vries H.E., Nicolaes G.A., Dijkstra C.D., Kooij G, & Lutgens E. (2017). Inhibition of CD40-TRAF6 interactions by the small molecule inhibitor 6877002 reduces neuroinflammation. Journal of Neuroinflammation, 14, 105.

+ Open protocol

+ Expand

Cytokine Secretion Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture supernatants were harvested and frozen at − 20 °C until further analysis. TNF-α, IL-8 and IL-12p70 secretion levels were determined using the respective Cytoset ELISA Kits (Invitrogen). IL-6 and IL-1β secretion were determined using the respective human Pelipair ELISA Kits (Sanquin, Amsterdam, The Netherlands). All ELISA assays were done according to the manufacturer’s protocol.

Klaver E., van der Pouw Kraan T., Laan L., Kringel H., Cummings R., Bouma G., Kraal G, & van Die I. (2015). Trichuris suis soluble products induce Rab7b expression and limit TLR4 responses in human dendritic cells. Genes and immunity, 16(6), 378-387.

+ Open protocol

+ Expand

Quantifying Cartilage and Synovium Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatants of cartilage and synovium cultures were analysed for matrix-metalloproteinase (MMP)1 and MMP3, tissue-inhibitorof-metalloproteinases (TIMP1), and pain-related mediators nervegrowth-factor (NGF) and vascular-endothelial-growth-factor (VEGF) using multiplex assays 41 . Some MMP3 concentrations measured were above the highest concentration of the standard. As the reproducibility of the assay for these high concentrations is good (coefficient of variation <3% for the three highest concentrations of the standard curve), these data could still be used in the analyses. The cytokines IL6 and IL8 were measured by ELISA (CytoSet ELISA Kit's; Invitrogen), according to manufacturer's instructions. All values were corrected for tissue weight and culture volume.

Steen-Louws C., Popov-Celeketic J., Mastbergen S.C., Coeleveld K., Hack C.E., Eijkelkamp N., Tryfonidou M., Spruijt S., van Roon J.A.G, & Lafeber F.P.J.G. (2018). IL4-10 fusion protein has chondroprotective, anti-inflammatory and potentially analgesic effects in the treatment of osteoarthritis. Osteoarthritis and cartilage, 26(8).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!