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Ifnγ apc cy7 clone 4s b3

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IFNγ-APC/Cy7 Clone 4S.B3 is a fluorescently-labeled monoclonal antibody used for the detection and quantification of interferon-gamma (IFNγ) in flow cytometry applications. The antibody is conjugated to the fluorescent dyes APC and Cy7, which allow for simultaneous multicolor analysis.

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Lab products found in correlation

Foxp3 tf staining buffer set, by Thermo Fisher Scientific (2 mentions) Monensin, by Thermo Fisher Scientific (2 mentions) Tnfα bv711 clone mab11, by BioLegend (2 mentions) Il2 pecy7 clone mq1 17h12, by BioLegend (2 mentions)

Close spelling variants of this product

APC-Cy7 (106 citations) IFNγ-APC-Cy7 (7 citations) Clone 4S.B3 (3 citations) APC-Cy7-conjugated anti-IFNγ (clone 4S.B3) (2 citations)

2 protocols using ifnγ apc cy7 clone 4s b3

1

Intracellular Cytokine Staining of CAR-T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ICS analysis, CAR+ T cells and target cells were plated at 1:1 E:T ratio in CM containing 1X Monensin (eBioscience) and 5uL/test CD107a antibody (BV605, Clone H4A3, BioLegend) for 5–6 hours. After incubation, intracellular cytokine staining was performed using the FoxP3 TF Staining Buffer Set (eBioscience) according to the manufacturer’s instruction using the following antibodies from BioLegend: IL2-PECy7 Clone MQ1–17H12, IFNγ-APC/Cy7 Clone 4S.B3, and TNFα-BV711 Clone Mab11.
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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2

Intracellular Cytokine Staining of CAR-T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ICS analysis, CAR+ T cells and target cells were plated at 1:1 E:T ratio in CM containing 1X Monensin (eBioscience) and 5uL/test CD107a antibody (BV605, Clone H4A3, BioLegend) for 5–6 hours. After incubation, intracellular cytokine staining was performed using the FoxP3 TF Staining Buffer Set (eBioscience) according to the manufacturer’s instruction using the following antibodies from BioLegend: IL2-PECy7 Clone MQ1–17H12, IFNγ-APC/Cy7 Clone 4S.B3, and TNFα-BV711 Clone Mab11.
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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