Antimousecd29

Manufactured by BioLegend

Sourced in United States

AntimouseCD29 is a monoclonal antibody product designed for flow cytometry applications. It recognizes the CD29 cell surface antigen, also known as the β1 integrin subunit, which is expressed on a variety of cell types. This product can be used to identify and characterize cells expressing CD29.

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Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (1 mentions) Anti mouse sca1, by BioLegend (1 mentions) Anti mouse igg peroxidase a4416, by Merck Group (1 mentions) Anti mouse cd45, by BioLegend (1 mentions) Mouse anti tg2 antibody, by Thermo Fisher Scientific (1 mentions) Anti mouse cd106, by BioLegend (1 mentions) Anti mouse cd73, by BioLegend (1 mentions) Antirabbit igg peroxidase a0545, by Merck Group (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions)

2 protocols using antimousecd29

Spheroid and Subspheroid Characterization Assay

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For spheroid and subspheroid assays, growth
supplements were used: B27 supplement (50×) (Gibco, 15504-044),
fibroblast-growth factor (100 μg/mL) (Gibco, AA-10-155), insulin
transferrin selenium (ITS) (Gibco, 41400-045), N2 supplement (100×)
(Gibco, 17502-048), and epithelial-growth factor (EGF) (200 μg/mL)
(Invitrogen, PHG0313); mouse-anti-TG2 antibody (CUB 7402, Thermo Fisher
Scientific, M S-224-P), monoclonal mouse anti-ITGβ1 antibody
(Santa Cruz, sc-8978), and anti-SDC-4 antibody (Abcam, ab24511). For
characterization, antimouse CD11b (BioLegend, B192967), antimouse
Sca-1 (BioLegend, B163257), antimouse CD44 (BioLegend, B193068), antimouse
CD45 (BioLegend, B187805), antimouse CD73 (BioLegend, B182619), antimouse
CD29 (BioLegend, B181560), and antimouse CD106 (BioLegend, B178443).
Antirabbit IgG peroxidase (A0545) and antimouse IgG peroxidase (A4416)
conjugates were purchased from Sigma.

Ulukan B., Bihorac A., Sipahioglu T., Kiraly R., Fesus L, & Telci D. (2020). Role of Tissue Transglutaminase Catalytic and Guanosine Triphosphate-Binding Domains in Renal Cell Carcinoma Progression. ACS Omega, 5(43), 28273-28284.

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Cell Sorting, Culturing, and Analysis

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Three-quarters of the total bulk cells were prepared for FACS, in order to obtain Sk-DN (CD45-/34-/29+) and Sk-34 (CD45-/34+) cells (FACS Aria III, Nippon BD, Japan). Other cells were also counted. Anti-mouse CD29, CD45 (Biolegend, San Diego, CA, USA), and CD34 (BD Pharmingen, San Jose, CA, USA) were used for cell sorting. As a result, four unsorted groups of cells were obtained: unsorted bulk, Sk-DN, Sk-34, and other cells. The bulk, Sk-DN, and Sk-34 cells were then cultured in 20% FCS/IMDM for 5 days, and their differentiation/proliferation capacities were examined. After culturing, the cells were harvested using 0.25% Trypsin-EDTA (Life Technologies Japan, Tokyo, Japan), washed with DMEM, and further prepared for cytospin and mRNA analysis as described below.

Fukuzawa T., Natsume T., Tamaki M., Imai T., Yamato I, & Tamaki T. (2022). Quantitative Evaluation of the Reduced Capacity of Skeletal Muscle Hypertrophy after Total Body Irradiation in Relation to Stem/Progenitor Cells. Journal of Clinical Medicine, 11(13), 3735.

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