1100 series autosampler hplc

Dried peptides were reconstituted in 15 μL of 0.1% formic acid in water. An Agilent 1100 series autosampler/HPLC was used to draw 0.5 μL of sample and inject it onto a C18 reverse phase column (GRACE; 150 × 0.500 mm) where an acetonitrile concentration gradient (5–30% in water with 0.1% formic acid) was used to elute peptides for online ESI-MS/MS by a QStar XL mass spectrometer (Applied Biosystems). Pertinent instrument scan settings were as follows: 4800 scans were taken during 140 min of elution for ions between 300 to 1800 m/z with charge states of 2 to 5 exceeding 10 counts. Former target ions were excluded for 300 s. Column cleaning was performed automatically with 2 cycles of a 5–85% acetonitrile gradient lasting 15 min each between runs.

Dried peptides were reconstituted in 15 µL of 0.1% formic acid in water. An Agilent 1100 series autosampler/HPLC was used to draw 0.5 µL of sample and inject it onto a C18 reverse phase column (GRACE; 150 × 0.500mm) where an acetonitrile concentration gradient (5–30% in water with 0.1% formic acid) was used to elute peptides for on-line ESI-MS/MS by a QStar XL mass spectrometer (Applied Biosystems). Column cleaning was performed automatically with 2 cycles of a 5–85% acetonitrile gradient lasting 15 min each between runs.