Human hypopharyngeal normal cell culture media with serum

Manufactured by Celprogen

Sourced in United States

Human Hypopharyngeal Normal Cell Culture Media with Serum is a cell culture media formulation designed to support the growth and maintenance of human hypopharyngeal normal cells in vitro. This media contains the necessary growth factors, nutrients, and serum components required for the optimal proliferation and survival of these specific cell types.

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Lab products found in correlation

Serum free media, by Celprogen (3 mentions) Gentamicin, by Thermo Fisher Scientific (2 mentions) Human epidermal growth factor (hegf), by Thermo Fisher Scientific (2 mentions) Kgm 2 sf, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

4 protocols using human hypopharyngeal normal cell culture media with serum

Establishment of Hypopharyngeal Cell Models

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Human hypopharyngeal primary cells (HHPC) were obtained from Celprogen Inc. CA, USA. The HHPC were plated in non-coated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA, USA), at 37°C in humidified air and 5% CO₂. Cells were sub-cultured and media were gradually replaced by Serum Free Media (Celprogen Inc. CA, USA).
We also established a telomerase-immortalized human hypopharyngeal cell line (HHK), by expression of hTERT, extending its life span without altering the characteristic phenotypic properties of the cells, as previously described [10 (link),33 (link)]. HHK, were grown in keratinocyte serum free basal medium (KGM-2 SF, Gibco^®) supplemented by L-Glutamine, BPE, hEGF and gentamicin (Gibco^®), at 37°C in humidified air and 5% CO₂.

Sasaki C.T., Toman J, & Vageli D. (2016). The In Vitro Effect of Acidic-Pepsin on Nuclear Factor KappaB Activation and Its Related Oncogenic Effect on Normal Human Hypopharyngeal Cells. PLoS ONE, 11(12), e0168269.

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Culturing Human Hypopharyngeal Cell Lines

Cited 2 times

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Human hypopharyngeal carcinoma cell, FaDu, was purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum (Gibco-BRL, Carlsbad, CA, USA) and penicillin-streptomycin at 37°C incubator according to previous study [15 (link)]. Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16 (link)].

Zheng Y., Su L., Tan J, & Dong F. (2022). Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1. Bioengineered, 13(3), 4911-4922.

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Cultivation of Human Hypopharyngeal Cells

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HHPC were provided by Celprogen Inc., Torrance, CA and cultured as previously described.17, 19 In detail, The HHPC were plated in noncoated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA), at 37°C in humidified air and 5% CO₂. The HHPC were subcultured and media were gradually replaced by Serum Free Media (Celprogen Inc.), and passed after reaching ~90% confluence, using 0.05% trypsin‐EDTA (Gibco^®, NY).

Doukas S.G., Cardoso B., Tower J.I., Vageli D.P, & Sasaki C.T. (2019). Biliary tumorigenic effect on hypopharyngeal cells is significantly enhanced by pH reduction. Cancer Medicine, 8(9), 4417-4427.

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Establishing Human Hypopharyngeal Cell Cultures

Cited 1 time

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We used human hypopharyngeal primary cells (HHPC) from Celprogen Inc. CA, USA. The HHPC were plated in non-coated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA, USA), at 37°C in humidified air and 5% CO2. The HHPC were sub-cultured and media were gradually replaced by Serum Free Media (Celprogen Inc. CA, USA), and passed after reaching ∼90% confluence, using 0.05% trypsin-EDTA (Gibco^®, NY, USA).
We also established a telomerase-immortalized human hypopharyngeal keratinocytes (HHK), by expression of hTERT, extending its life span without altering the characteristic phenotypic properties of the cells, as previously described [20 (link)]. HHK, were grown in keratinocyte serum free basal medium (KGM-2 SF, Gibco^®, NY, USA) supplemented by L-Glutamine, BPE, hEGF and gentamicin (Gibco^®, NY, USA), at 37°C in humidified air and 5% CO2.

Vageli D.P., Doukas S.G, & Sasaki C.T. (2017). Inhibition of NF-κB prevents the acidic bile-induced oncogenic mRNA phenotype, in human hypopharyngeal cells. Oncotarget, 9(5), 5876-5891.

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