Log In Sign up
The largest database of trusted experimental protocols

Anti sirt1 sc 15404

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in France, United States

Anti-SIRT1 (sc-15404) is a primary antibody product from Santa Cruz Biotechnology. It is designed to detect SIRT1 (Sirtuin 1) protein expression.

Automatically generated - may contain errors

Lab products found in correlation

Anti flag antibody f1804, by Merck Group (2 mentions) Immobilon western blotting kit, by Merck Group (2 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Anti phospho p65 s536 3031 antibodies, by Cell Signaling Technology (1 mentions) 3 3 diaminobenzidine (dab), by ZSGB-BIO (1 mentions) D glucose, by Merck Group (1 mentions)

4 protocols using anti sirt1 sc 15404

1

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were lysed in 10 mM Tris (pH 7.4), 150 mM sodium chloride, 1 mM EDTA, 0.5% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and protease inhibitor mixture (Roche Applied Science). Samples were separated using SDS–PAGE and transferred onto nitrocellulose membranes. The anti-β-tubulin (sc-9104), anti-RelB (sc-226), anti-p65 (sc-372), anti-p105/p50 (sc-8414), anti-IκBα (sc-371), and anti-SIRT1 (sc-15404) antibodies (Santa Cruz Biotechnology); anti-Lamin A/C (2032), anti-myc (2276), and anti-p52 (4882) (Cell Signaling); anti-YY1 (A302-779A) (Bethyl Laboratories), and anti-flag (F1804) antibodies (Sigma-Aldrich) were used. Antigen–antibody complexes were visualized by ECL using Immobilon Western blotting kit (Millipore).
Waters M.R., Gupta A.S., Mockenhaupt K., Brown L.N., Biswas D.D, & Kordula T. (2019). RelB acts as a molecular switch driving chronic inflammation in glioblastoma multiforme. Oncogenesis, 8(6), 37.
+ Open protocol
+ Expand
2

Western Blot Analysis of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 10 mM Tris (pH 7.4), 150 mM sodium chloride, 1 mM EDTA, 1% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and Pierce protease inhibitor mixture. Samples were separated on a 10% gel, and transferred onto nitrocellulose membranes (GE Healthcare). Anti-β-tubulin (sc-9104), anti-RelB (sc-226), anti-p65 (sc-372), anti-p105/p50 (sc-8414), anti-IκBα (sc-371), and anti-SIRT1 (sc-15404) antibodies (Santa Cruz Biotechnology); anti-Lamin A/C (2032), anti-myc (2276) anti-GAPDH (5174), and anti-phospho-p65(S536) (3031) antibodies (Cell Signaling); anti-flag (F1804) antibodies (Sigma-Aldrich); and anti-pRelB(S472) antibodies were from CliniSciences (NB-22-1004) (Nanterre, France). Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
Gupta A.S., Waters M.R., Biswas D.D., Brown L.N., Surace M.J., Floros C., Siebenlist U, & Kordula T. (2019). RelB controls adaptive responses of astrocytes during sterile inflammation. Glia, 67(8), 1449-1461.
+ Open protocol
+ Expand
3

Evaluation of Chemotherapy Drug Resistance

Check if the same lab product or an alternative is used in the 5 most similar protocols
D-Glucose and methylthiazoly-diphenyl-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The chemotherapy drug 5-fluorouracil (5-FU) was acquired from The First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University (Xi’an, China). The primary antibodies used were as follows: Rabbit anti-human polyclonal anti-Nampt (AV42255; Sigma-Aldrich), rabbit anti-human polyclonal anti-p53 (sc-126; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA)/p-glycoprotein (P-gp; sc-55510; Santa Cruz Biotechnology, Inc.), rabbit anti-human polyclonal anti-topoisomerase (topo)-IIα (20233-1-AP; Proteintech, Chicago, IL, USA) and rabbit anti-human monoclonal anti-Sirt1 (sc-15404; Santa Cruz Biotechnology, Inc.). The secondary antibodies and 3,3′-diaminobenzidine were purchased from ZSGB-BIO (Beijing, China).
ZHAO W., CHEN R., ZHAO M., LI L., FAN L, & CHE X.M. (2015). High glucose promotes gastric cancer chemoresistance in vivo and in vitro. Molecular Medicine Reports, 12(1), 843-850.
+ Open protocol
+ Expand
4

Dual-Immunostaining for SIRT1 and ISG15

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Dual-immunohistochemical staining for SIRT1 and ISG15 was performed using a Ventana BenchMark ULTRA system (Roche Korea Diagnostics Ltd., Seoul, Korea). Antigen retrieval was performed for 64 min using pH 9.0 CC1 antigen retrieval solution (Roche Korea Diagnostics Ltd.). The specimens were incubated with primary antibodies (anti-SIRT1 (sc-15404), 1:100, Santa Cruz; and anti-ISG15 (HPA004627), 1:100, Atlas Antibodies) for 90 min, and developed with an OptiView DAB IHC Detection Kit (Roche Korea Diagnostics Ltd.) for SIRT1 and with an UltraView Universal Alkaline Phosphatase Red Detection Kit (Roche Korea Diagnostics Ltd.) for ISG15. For quantification, the combined expression of SIRT1 and ISG15 (SIRT1/ISG15) and the individual expression of SIRT1 or ISG15 were assessed. The combined expression of SIRT1 and ISG15 (SIRT1/ISG15) and the individual expression of SIRT1 or ISG15 were scored by summing the staining intensity score (0; no staining, 1; weak staining, 2; intermediate staining, 3; strong staining) and the area score (0; 0%, 1; 1%, 2; 2–10%, 3; 11–33%, 4; 34–66%, 5; 67–100%)40 (link). The final score was assessed by summing the scores from the two tissue microarray cores, resulting in a final immunohistochemical staining score ranging from zero to sixteen41 (link).
Kang J.A., Kim Y.J., Jang K.Y., Moon H.W., Lee H., Lee S., Song H.K., Cho S.W., Yoo Y.S., Han H.G., Kim M.J., Chung M.J., Choi C.Y., Lee C., Chung C., Hur G.M., Kim Y.S, & Jeon Y.J. (2024). SIRT1 ISGylation accelerates tumor progression by unleashing SIRT1 from the inactive state to promote its deacetylase activity. Experimental & Molecular Medicine, 56(3), 656-673.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!