Garcinol

Histone acetyltransferase inhibitor Garcinol (GAR), SIRT1 activator Resveratrol (RES), SIRT1 inhibitor Nicotinamide (NIC) and NF-κB inhibitor BAY 11-7082 (BAY) were obtained from Santa Cruz (Santa Cruz, CA). Antibodies against HMGB1 and β-actin were purchased from Cell Signaling Technology (Danvers, MA). Anti-acetyl lysine antibody (clone Kac-01) was purchased from PTM Biolab (Hangzhou, China). siRNAs were purchased from biotend (Shanghai, China). The bovine serum albumin (BSA) was purchased from Dingguo (Nanjing, China).

Cells were pre-treated with Tip60 inhibitors, MG149 (Axon MedChem) or NU9056 (Tocris Bioscience), or general HAT inhibitors, curcumin (Santa Cruz Biotech) or garcinol (Santa Cruz Biotech), for 6 h. KSHV reactivation was induced by treating HEK293T.Bac36 cells with 20 ng/mL 12-O-Tetradecanoylphorbol-13-acetate (TPA) (Sigma-Aldrich) and 1.5 mM sodium butyrate (SB) (Sigma-Aldrich). For MC116.219, BCBL-1, and BC-3 cells, 0.3 mM of SB was used instead.

Garcinol (sc-200891A, HPLC purity ≥95%) and Z-VAD-FMK (sc-3067, HPLC purity ≥95%) purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) was dissolved in dimethyl sulfoxide (DMSO) to prepare a 20 mM stock and stored at −20 °C until use. For different assays, the stock was further diluted using cell growth medium as appropriate. Dimethyl sulfoxide (DMSO), served as vehicle and negative control. BD Pharmingen™ PE Annexin V apoptosis detection kit I (#559763) was purchased from BD Biosciences (San Jose, CA, USA). Unless otherwise indicated, all reagents were obtained from Gibco (Thermo Fisher Scientific, Life Technologies, Foster City, CA, USA).