Abi q6 flex real time pcr machine

Manufactured by Thermo Fisher Scientific

Sourced in United States, Japan

The ABI Q6 Flex Real-Time PCR machine is a laboratory instrument designed for performing real-time polymerase chain reaction (PCR) analysis. It is capable of accurately detecting and quantifying target DNA sequences in real-time.

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Lab products found in correlation

Rnaiso plus, by Takara Bio (2 mentions) Hiscript 3 rt supermix for qpcr gdna wiper kit, by Vazyme (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Sybr green pcr master mix, by Takara Bio (1 mentions)

Close spelling variants of this product

PCR machine (27 citations) ABI Q6 (14 citations)

2 protocols using abi q6 flex real time pcr machine

Quantitative Real-Time PCR Procedure

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Total RNA was isolated using the RNAiso Plus (9109, Takara, Tokyo, Japan). 0.5 mg of total RNA was reverse transcribed into complementary DNA using the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (R323, Vazyme, Nanjing, China). Then the Q-PCR was performed on the ABI Q6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA) using the 2x SYBR Green qPCR Master Mix kit (B21203, Bimake, Houston, Texas USA). The relative gene expression levels were analyzed using the 2(–ΔΔCt) method and normalized against 18 S expression. Primer sequences are in Supplementary Table 2.

Wu Y., Huang T., Li X., Shen C., Ren H., Wang H., Wu T., Fu X., Deng S., Feng Z., Xiong S., Li H., Gao S., Yang Z., Gao F., Dong L., Cheng J, & Cai W. (2023). Retinol dehydrogenase 10 reduction mediated retinol metabolism disorder promotes diabetic cardiomyopathy in male mice. Nature Communications, 14, 1181.

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Cardiac Gene Expression Quantification

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Total RNA was isolated from the heart tissues using the RNAiso Plus according to the manufacturer's instruction (Takara, Tokyo , Japan). Five hundred nanograms of total RNA was reverse transcribed into complementary DNA in a 10 μl reaction mixture using the PrimeScriptTM RT reagent kit (Takara, Tokyo, Japan). The Q-PCR was performed using the SYBR Green PCR Master Mix (Takara, Tokyo, Japan) and the ABI Q6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). The relative gene expression levels were analyzed using the 2(-ΔΔCt) method and normalized against GAPDH expression. The primers used are listed in Table S2.

Li X., Wu Y., Zhao J., Wang H., Tan J., Yang M., Li Y., Deng S., Gao S., Li H., Yang Z., Yang F., Ma J., Cheng J, & Cai W. (2020). Distinct cardiac energy metabolism and oxidative stress adaptations between obese and non-obese type 2 diabetes mellitus. Theranostics, 10(6), 2675-2695.

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