Chicken anti insulin

Manufactured by Abcam

Sourced in United States

Chicken anti-insulin is a primary antibody that recognizes insulin, a hormone produced by the pancreas that regulates blood sugar levels. This antibody can be used in various immunoassay techniques to detect and quantify insulin in biological samples.

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Lab products found in correlation

Guinea pig anti glucagon, by Merck Group (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Fv1000 d confocal laser scanning microscope, by Olympus (1 mentions) Guinea pig anti insulin, by Agilent Technologies (1 mentions) Bz x analyzer, by Keyence (1 mentions) Anti ki67, by BD (1 mentions)

Close spelling variants of this product

Anti-insulin Insulin

2 protocols using chicken anti insulin

Immunofluorescence Staining of Hormones

Cited 1 time

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Immunofluorescence was carried out as previously described [17 (link)] using commercial antisera against glucagon (guinea pig anti-glucagon at 1:7000; EMD Millipore, Billerica MA), somatostatin (sheep anti-somatostatin at 1:1000; American Research Products Inc., Waltham MA), insulin (chicken anti-insulin at 1:1000; Abcam) and IL1r1/CD121a (armenian hamster anti-mouse Il1r1/CD121a-APC at 1:100; Biolegend, San Diego, CA). Rabbit antisera against UCN3 (#7218, 1:2000) and CRH (rc70, 1:5000) were generated in house. Secondary antibodies were raised in donkey against each of these host species and were obtained from Jackson Immuno Research Laboratories (West Grove, PA) conjugated to Alexa Fluor-405, -488, -649 or Cy3 and used at 1:600. Dapi was applied as a nuclear stain at 1 microgram/ml and slides were embedded with Prolong Gold antifade reagent (Life Technologies, Carlsbad, CA). All imaging was carried out on Zeiss LSM780 confocal microscopes at The Waitt Advanced Biophotonics Center Core facility of the Salk Institute.

Benner C., van der Meulen T., Cacéres E., Tigyi K., Donaldson C.J, & Huising M.O. (2014). The transcriptional landscape of mouse beta cells compared to human beta cells reveals notable species differences in long non-coding RNA and protein-coding gene expression. BMC Genomics, 15(1), 620.

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Immunohistochemical Evaluation of Pancreatic Islets

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Immunohistochemistry was performed for the observation of GFP-expressing cells, as described previously [14 (link)]. The primary antibodies used in this study were the following: guinea pig anti-insulin (1:1000; Dako, Carpinteria, CA, USA), chicken anti-insulin (1:1000; Abcam, Cambridge, MA, USA), rabbit anti-GFP (1:200; MBL, Nagoya, Japan), and anti-Ki67 (1:100; BD Pharmingen, Franklin Lakes, NJ, USA). Insulin-positive area and total pancreas area were measured using a BZ-X analyzer (Keyence, Osaka, Japan), and β-cell mass was calculated as relative β-cell area multiplied by the pancreatic weight. For the detection of Ki67, mounted sections were microwaved at 95 °C for 20 min in citrate buffer (pH 6.0) for antigen retrieval, before being incubated with blocking serum. Slides were observed using an Olympus FV1000D confocal laser-scanning microscope. In order to detect apoptotic cells, TUNEL assay was performed using an apoptotic detection system (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s protocol.

Kubo F., Miyatsuka T., Sasaki S., Takahara M., Yamamoto Y., Shimo N., Watada H., Kaneto H., Gannon M., Matsuoka T.A, & Shimomura I. (2016). Sustained expression of GLP-1 receptor differentially modulates β-cell functions in diabetic and nondiabetic mice. Biochemical and biophysical research communications, 471(1), 68-74.

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