Paclitaxel, doxorubicin, etoposide, LPS, ATP, nigericin, staurosporine, glibenclamide, and valiomycin were purchased from Sigma-Aldrich. Ciliobrevin D and SP600125 were purchased from Calbiochem. JC-1 and MitoSOX were purchased from Invitrogen. Alum was purchased from InvivoGen. Anti-mouse caspase-1, anti-NLRP3 and anti-ASC antibodies were obtained from AdipoGen. Anti-mouse IL-1β antibody was obtained from R&D Systems. Anti-phospho-IκB and anti- IκB antibodies were purchased from Cell Signaling Technology. Anti-phospho-JNK and anti-JNK antibodies were obtained from Invitrogen and BD, respectively. Anti-β-actin antibody was purchased from Santa Cruz Biotechnology.
Anti phospho jnk
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-phospho-JNK is a laboratory reagent used to detect and quantify the phosphorylated form of the c-Jun N-terminal kinase (JNK) protein. JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a crucial role in cellular signaling pathways. The Anti-phospho-JNK reagent can be used in various applications, such as Western blotting, immunohistochemistry, and ELISA, to analyze the activation status of JNK in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti jnk, by Cell Signaling Technology (2 mentions)
Nigericin, by Merck Group (1 mentions)
Glibenclamide, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Anti mouse il 1β antibody, by R&D Systems (1 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Anti mouse caspase 1, by Adipogen (1 mentions)
Anti iκb antibodies, by Cell Signaling Technology (1 mentions)
Mitosox, by Thermo Fisher Scientific (1 mentions)
Anti phospho iκb, by Cell Signaling Technology (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Etoposide, by Merck Group (1 mentions)
Staurosporine, by Merck Group (1 mentions)
Anti nlrp3, by Adipogen (1 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Sulfanilamide, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
5 protocols using anti phospho jnk
1
Inflammasome Activation and Inhibition
2
Apoptosis Mechanism Biochemical Assays
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Hydrogen peroxide (H2O2), N-acetyl-cysteine (NAC), N-(1-naphthyl)ethylenediamine dihydrochloride, sulfanilamide, 2-methyl-2-thiopseudourea hemisulfate salt, sodium nitrite, orthophosphoric acid, and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich Chemical Co. (Saint Louis, MO). 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolylcarbocyanine chloride (JC-1), 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), ROS-sensitive dichlorodihydrofluorescin diacetate (H2DCFDA), 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM diacetate), and MitoTrackerTM were purchased from Invitrogen (Carlsbad, CA). SP600125 and SB203580 were obtained from Calbiochem (San Diego, CA). Fluorescent mounting medium was purchased from Dako (Carpinteria, CA). Primary antibodies used in this study include anti-Bax, anti-JNK, anti-extracellular signal-related kinase (ERK), anti-phospho-ERK, anti-p38, anti-phospho-p38, anti-caspase 3, anti-bid, anti-Bcl-xL, anti-phospho-Bcl2, anti-cytochrome C, (Cell Signaling Technology, Beverly, MA), and anti-phospho-JNK (Invitrogen, CA), anti-actin and anti-COX IV (Abcam, Cambridge, UK), and anti-PARP (BD Bioscience, San Jose, CA) antibodies. Horseradish peroxidase (HRP)-conjugated and fluorescently labeled Alexa488- and Alexa546-conjugated antibodies (Invitrogen) were used as secondary antibodies.
3
UV/IR Spectral and NMR Analysis
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The UV and IR spectra A were obtained using Jasco UV-550 and Perkin-Elmer model LE599 spectrometer, respectively. A Bruker DRX 500 or 700 MHz spectrometer were used for the analysis of NMR signals using methanol-d4 as solvents. ESIMS data were measured on VG Autospec Ultima Mass spectrometers.
A RAW264.7 mouse macrophage cell line was obtained from Korean Cell Line Bank (No. 40071, Seoul, Korea), and a HaCaT human keratinocyte cell line was obtained from Cell Lines Service GmbH (No. 300493, Eppelheim, Germany). Griess reagent was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). A TaqMan probe was obtained from Applied Biosystems (Foster City, CA, USA). To confirm the change in protein level, anti-IκB-α, anti-phospho-IκB-α, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK and anti-GAPDH were obtained from Invitrogen (Carlsbad, CA, USA) and anti-p38 MAPK, anti-phospho-p38 MAPK, anti-STAT3 and anti-phospho-STAT3 were purchased from Cell Signaling (Danvers, MA, USA).
A RAW264.7 mouse macrophage cell line was obtained from Korean Cell Line Bank (No. 40071, Seoul, Korea), and a HaCaT human keratinocyte cell line was obtained from Cell Lines Service GmbH (No. 300493, Eppelheim, Germany). Griess reagent was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). A TaqMan probe was obtained from Applied Biosystems (Foster City, CA, USA). To confirm the change in protein level, anti-IκB-α, anti-phospho-IκB-α, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK and anti-GAPDH were obtained from Invitrogen (Carlsbad, CA, USA) and anti-p38 MAPK, anti-phospho-p38 MAPK, anti-STAT3 and anti-phospho-STAT3 were purchased from Cell Signaling (Danvers, MA, USA).
4
Protein Expression Analysis in Cell Lysates
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Cells were lysed in RIPA lysis buffer (50 mM Tris–HCl, 150 mM NaCl, 1 mM EDTA-Na2, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitors and phosphatase inhibitor on ice for 15 min. The cell lysates were cleared by centrifugation at 12,000×g for 10 min, then heat-denatured with 5 × Laemmli buffer (G2013, Servicebio). The prepared samples with equal amount of total protein (20 μg) were subjected to SDS-PAGE and immunoblotting, as per the standard procedure. The following antibodies were used: anti-SOX1 (ab109290, Abcam), anti-β-catenin (ab32572, Abcam), anti-HES1 (#11988, Cell Signaling Technology [CST]), anti-PROX1 (11067-2-AP, Proteintech), anti-p38 (#8690, CST), anti-phospho-p38 (#9215, CST), anti-ERK (#4695, CST), anti-phospho-ERK (#4370, CST), anti-AKT (#4691, CST), anti-phospho-AKT (#4060, CST), anti-JNK (#9252, CST), anti-phospho-JNK (#700031, Invitrogen), anti-RAF (#9422, CST), anti-phospho-RAF (#9427, CST), anti-MEK (51080-1-AP, Proteintech), anti-phospho-MEK (#9154, CST), anti-α-Tubulin (11224-1-AP, Proteintech), anti-GAPDH (BM1623, Boster), anti-BCL2 (Proteintech, 12789-1-AP), anti-PCNA (BM0104, Boster). All antibodies were diluted by antibody dilutions (G2025, Servicebio) with a ratio of 1:1,000. All quantitative analyses were performed using the software image J.
5
Western Blot Analysis of Cellular Proteins
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Total protein cell extracts were separated by SDS–polyacrylamide gel electrophoresis gels and transferred to Immobilon-P polyvinylidene difluoride membranes (Millipore). Membranes were probed with primary antibodies; anti-p53(1C12) (Cell Signaling; 2524; 1:750 dilution), anti-p21(C-19) (Santa Cruz Biotechnology; sc-397; 1:1,000 dilution), anti-cleaved caspase-3 (Asp175) (Cell Signaling; 9661; 1:1,000 dilution), anti-phospho JNK (Invitrogen; 44-682G; 1:1,000 dilution), anti-JNK (Cell Signaling; 9252; 1:1,000 dilution), anti-IκBα (Santa cruz; sc-371; 1:1,000 dilution), anti-tubulin (Sigma; T6074; 1:5,000) and anti-actin (Santa Cruz Biotechnology; sc-1616; 1:1,000 dilution) antibodies at 4° O/N. Membranes were incubated with secondary horseradish peroxidase-coupled antibodies (GE Healthcare and Jackson Immune Research) and developed with chemiluminescent detection substrate (GE Healthcare and Thermo Scientific).
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