Serum alanine transaminase (ALT) and aspartate transaminase (AST) were determined using a clinical automatic analyzer (Hitachi, Tokyo, Japan) and a commercial reagent kit (Roche Diagnostic, Mannheim, Germany) according to the manufacturer’s protocol.
Clinical automatic analyzer
Manufactured by Hitachi
Sourced in Japan
The Clinical Automatic Analyzer is a laboratory equipment designed to perform automated analysis of clinical samples. The core function of this device is to automate the process of measuring and analyzing various components in biological samples, such as blood, urine, or other bodily fluids.
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Lab products found in correlation
6 protocols using clinical automatic analyzer
1
Serum Enzyme Measurements: ALT and AST
2
Quantifying Hepatic Function and Oxidative Stress
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By conferring to industrialist’s procedure, and consuming a clinical automatic analyzer (Hitachi, Japan) and a commercial reagent kit (Roche Diagnostic, Mannheim, Germany), levels of Serum ALT, AST, and TBIL were quantified. Hepatic quantity was quantified conferring to the company’s protocol with hydroxyproline detection kit (Sigma-Aldrich, St. Louis, Mo, USA). GSH, GSSG, SOD, MDA, IL-1β, and TNF-α intensities were quantified via commercial kits (Lipid peroxidation). The quantity of oxidative stress damage was evaluated by the proportions GSH:GSSG. According to the producers’ protocols all the processes were accomplished.
3
Serum Transaminase Measurement Protocol
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Serum samples were separated from blood by centrifugation at 3000 ×g for 15 min at 4°C. Serum alanine transaminase (ALT) and aspartate transaminase (AST) were determined by using a clinical automatic analyzer (Hitachi, Japan) and commercial reagent kit (Roche Diagnostic, Mannheim, Germany) according to the manufacturer's protocol.
4
Quantifying Liver Enzymes and Oxidative Stress
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By conferring to industrialist’s procedure, and consuming a clinical automatic analyzer (Hitachi, Japan) and a commercial reagent kit (Roche Diagnostic, Mannheim, Germany), levels of ALT, AST were quantified in the serum. Hepatic quantity was quantified conferring to the company’s protocol with hydroxyproline detection kit (Sigma-Aldrich, St. Louis, MO, USA). GSH, SOD, MDA intensities were quantified via commercial kits (lipid peroxidation). According to the producers’ protocols all the processes were accomplished.
5
Metabolic Profile Evaluation Protocol
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Serum biochemical parameters including TG, LDL-C, HDL-C, cholesterol, AST, and ALT were analyzed using an automatic clinical analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). Serum glucose concentrations were determined using a glucometer Rightest GM550 (Bionime). Serum levels of insulin, adiponectin, leptin, LPS, and MCP-1 were measured using commercial rat ELISA kits (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
6
Serum Biochemical and Glucose Tolerance Evaluation
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Serum biochemical parameters including triglyceride (TG), low density lipoprotein (LDL), cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were analyzed using an automatic clinical analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). Serum glucose concentrations were determined using a glucometer (TaiDoc Technology Co., Taipei, Taiwan). Serum levels of insulin were measured using a commercial rat insulin ELISA kit (Millipore, MA, USA) according to the manufacturer's instructions. Homeostasis model assessment (HOMA) was computed with the formula: HOMA = (FPI × FPG)/22.5, where FPI is fasting plasma insulin and FPG is fasting plasma glucose. 20 2.6. Oral glucose tolerance test (OGTT)
An oral glucose tolerance test was performed after 11 weeks treatment in this study. The diets were removed from animal cages for 12 h before the administration of an oral glucose load (2 g per kg of body weight). Blood samples were collected from the tail vein at 0, 30, 60, 90, and 120 min after glucose administration. Glucose concentrations were determined by using a glucometer (TaiDoc Technology Co., Taipei, Taiwan). The total glucose areas under the curve (AUC glucose ) between 0 and 120 min represented the magnitude of the glucose response and were calculated as described previously. 19
An oral glucose tolerance test was performed after 11 weeks treatment in this study. The diets were removed from animal cages for 12 h before the administration of an oral glucose load (2 g per kg of body weight). Blood samples were collected from the tail vein at 0, 30, 60, 90, and 120 min after glucose administration. Glucose concentrations were determined by using a glucometer (TaiDoc Technology Co., Taipei, Taiwan). The total glucose areas under the curve (AUC glucose ) between 0 and 120 min represented the magnitude of the glucose response and were calculated as described previously. 19
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