The cells were fixed in 4% formaldehyde diluted in PBS for 15 min, permeabilized with 0.3% Triton X-100, treated with blocking buffer (5% BSA in PBS), and then incubated overnight with the primary antibody at 4°C. The cells were then incubated with Red-conjugated secondary antibody from Jackson Immunoresearch Laboratories (West Grove, PA, USA) for 1 h at room temperature. Slides were mounted and examined using a Zeiss Instruments microscope (Carl Zeiss, Oberkochen, Germany).
Red conjugated secondary antibody
Manufactured by Jackson ImmunoResearch
Sourced in United States
Red-conjugated secondary antibody is a laboratory reagent used in immunoassays and other immunological techniques. It is designed to detect and visualize primary antibodies by binding to their constant regions and producing a red-colored signal. The red fluorescent label allows for the identification and localization of target proteins or antigens in samples.
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Lab products found in correlation
3 protocols using red conjugated secondary antibody
1
Immunostaining of Cells with Antibodies
2
Immunofluorescence Staining Protocol
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Cells were fixed in 4% formaldehyde diluted in phosphate buffered saline (PBS) for 15 min, permeabilized with 0.3% Triton X-100, treated with blocking buffer (5% BSA in PBS), and then incubated overnight with the primary antibody at 4 °C. Cells were then incubated with the Red conjugated secondary antibody from Jackson Immunoresearch Laboratories (West Grove, PA, USA) for 1 h at room temperature. Slides were mounted and examined using a Zeiss Instruments confocal microscope.
3
Immunofluorescence Microscopy of Cells
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Cells were fixed in 4% formaldehyde diluted in phosphate-buffered saline (PBS) for 15 min, permeabilized with 0.3% Triton X-100, treated with blocking buffer (5% BSA in PBS), and then incubated overnight with the primary antibody at 4°C. The cells were then incubated with the Red-conjugated secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) for 1 h at room temperature. Slides were mounted and examined using a Zeiss Instruments confocal microscope.
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