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1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc

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1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) is a water-soluble carbodiimide used as a coupling agent in organic synthesis and biochemical reactions. It facilitates the formation of amide bonds between carboxyl groups and primary amines.

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Lab products found in correlation

N hydroxysuccinimide nhs, by Thermo Fisher Scientific (3 mentions) N n dicyclohexylcarbodiimide dcc, by Merck Group (2 mentions) 5 norbornene 2 carboxylic acid, by Merck Group (2 mentions) Lithium phenyl 2 4 6 trimethylbenzoylphosphinate lap, by Merck Group (2 mentions) Streptavidin hrp conjugate, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions) N hydroxysulfosuccinimide sulfo nhs, by Thermo Fisher Scientific (1 mentions) N isopropylacrylamide nipam, by Merck Group (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Polyvinyl alcohol pva mw 25 000 16 000, by Merck Group (1 mentions) Pyridine, by Thermo Fisher Scientific (1 mentions) Dichloromethane dcm, by Thermo Fisher Scientific (1 mentions) Tetrazine amine, by Vector Laboratories (1 mentions) Medium m3 base, by INCELL (1 mentions) Thiazolyl blue tetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions) Phosphotungstic acid pta, by Electron Microscopy Sciences (1 mentions) U87 mg cell lines, by RIKEN BioResource Center (1 mentions) Dulbecco s modified eagle medium dmem powder, by Thermo Fisher Scientific (1 mentions) Bend 3, by RIKEN BioResource Center (1 mentions)

Close spelling variants of this product

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (14 citations) 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (2 citations)

12 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc

1

Multifunctional Nanoparticle Biosensing

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N-(4-Aminobutyl)-N-ethylisoluminal (ABEI), sodium sulfide nonahydrate (SDS), potassium persulfate (KPS), N, N’-Methylenebisacrylamide (bis), acrylic acid (AA), allylamine (ALA) and N-isopropylacrylamide (NIPAM) were purchased from Sigma Aldrich. The biotinylated mouse anti-human ErbB2/HER2 (Recombinant Monoclonal Human IgG1 Clone Hu5), mouse anti-human CD63 (Clone mem-259), and biotinylated mouse anti-human CD63 (Clone NVG-2) was obtained from R&D systems, Sino Biological, and BioLegend, respectively. Horseradish peroxidase (HRP)-conjugated secondary antibody was from Cell Signaling Technology; and the streptavidin-HRP-conjugate was attained from Invitrogen. All other chemicals, including 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysulfosuccinimide (Sulfo-NHS), were purchased from ThermoFisher Scientific.
Jiang Q., Liu Y., Wang L., Adkins G.B, & Zhong W. (2019). Rapid Enrichment and Detection of Extracellular Vesicles Enabled by CuS-Enclosed Microgels. Analytical chemistry, 91(24), 15951-15958.
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2

Daunorubicin and Doxorubicin Nanoparticles

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Daunorubicin hydrochloride and doxorubicin hydrochloride were provided by Jubilant Chemsys Ltd. Noida, Uttar Pradesh, India (purity ≥ 98%). PLGA was purchased from Supreme Combine, Mumbai, India and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Thermo Scientific. Dichloromethane (DCM) was obtained from Qualigens Fine Chemical, Mumbai, India. Polyvinyl alcohol (PVA, MW-25,000; 16,000), and sodium tripolyphosphate (TPP), ammonium formate (MS grade), acetonitrile and methanol (LC–MS grade), ammonium acetate (MS grade) and formic acid (purity > 98%) were obtained from Sigma-Aldrich (St Louis, MO). Water used was purified through Milli-Q water purification system (Millipore, Bedfrod, MA).
Ahmad N., Ahmad R., Alam M.A, & Ahmad F.J. (2018). Enhancement of oral bioavailability of doxorubicin through surface modified biodegradable polymeric nanoparticles. Chemistry Central Journal, 12, 65.
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3

Synthesis of Multifunctional PEG-Based Hydrogels

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The hydroxyl-terminated 8-arm PEG (20 kDa)
and 4-arm PEG-amino succinic acid (PEG-ASA) (10 kDa) were purchased
from JenKem Technology and Laysan Bio Inc., respectively. Carbic anhydride,
pyridine, dichloromethane (DCM), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (EDC) were all purchased from Thermo Scientific. 5-Norbornene-2-carboxylic
acid, tetrahydrofuran (THF), 4-dimethylaminopyridine (DMAP), and N,N′-dicyclohexylcarbodiimide (DCC)
were obtained from Sigma-Aldrich. Tetrazine-amine and methylTetrazine-amine
were purchased from Click Chemistry Tools. N-hydroxylsuccinimide
(NHS) and N,N-diisopropylethylamine
(DIEA) were obtained from Tokyo Chemical Industry (TCI). N,N-dimethylformamide (DMF), 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide
hexafluorophosphate (HATU), and cold soluble gelatin were purchased
from Alfa Aesar, AnaSpec, and Modernist Pantry, respectively. Calcein-AM
and ethidium homodimer stains were obtained from Biotium. F-actin
stain was purchased from Cytoskeleton, Inc.
Dimmitt N.H., Arkenberg M.R., de Lima Perini M.M., Li J, & Lin C.C. (2022). Hydrolytically Degradable PEG-Based Inverse Electron Demand Diels–Alder Click Hydrogels. ACS Biomaterials Science & Engineering, 8(10), 4262-4273.
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4

Synthesis and Characterization of PEG-PGlu Copolymers

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The poly(ethylene glycol)-b-poly(L-glutamic acid) diblock copolymer, PEG114-PGlu150 (ĐM = 1.01) was purchased from Alamanda Polymers, Inc. (Madison, AL, USA). PEG114-PGlu80 copolymer (ĐM = 1.07) was synthesized according to previously described procedure [17 (link)]. 7 - ethyl - 10 - hydroxylcamptothecin (SN38) for the conjugation (> 98%) and biological study (> 99.5%) were supplied by Acros Organics (Thermo Fisher, NJ, USA) and Selleckchem (Houston, TX, USA), respectively. Dichloro-(1, 2- diaminocyclohexane)-platinum (II) (DACHPt), MTT reagent (3- (4, 5- Dimethylthiazol- 2- yl)- 2, 5- diphenyltetrazolium bromide), 1, 2- ethylenediamine (ED), 4- dimethylaminopyridine (DMAP) were obtained from Sigma-Aldrich (St Louis, MO, USA). 1- (3- dimethylaminopropyl)- 3- ethylcarbodiimide hydrochloride (EDC) was purchased from Alfa Aesar (Haverhill, MA, USA). Fetal bovine serum (FBS), trypsin, Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Invitrogen Inc. (Carlsbad, CA, USA). Medium M3 Base was obtained from Incell Corp. (San Antonio, TX, USA). All reagent grade chemicals purchased from Sigma-Aldrich were used without any purification.
Lei F., Xi X., Rachagani S., Seshacharyulu P., Talmon G.A., Ponnusamy M.P., Batra S.K, & Bronich T.K. (2020). Nanoscale Platform for Delivery of Active IRINOX to Combat Pancreatic Cancer. Journal of controlled release : official journal of the Controlled Release Society, 330, 1229-1243.
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5

Synthesis and Characterization of PLGA Nanoparticles

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Poly(d,l-lactide-co-glycolide) 50:50 was from Evonik Industries (Birmingham, AL, USA). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-ethyldiisopropylamine (N,N-diisopropylethylamine) (DIEA, 99%), thiazolyl blue tetrazolium bromide (MTT, 98%) were from Alfa Aesar (Heysham, England). N-hydroxysuccinimide (NHS, 98%) was from Acros Organics Co. Inc. (Fair Lawn, NJ, USA). Cy5.5-NHS was from Lumiprobe Corporation (Hallandale Bench, FL, USA). T7-FITC peptide (MW 1395.57 g/mol, FITC-Asp-His-Ala-Ile-Tyr-Pro-Arg-His-OH) and R9-peptide (MW 1422.3 g/mol Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-OH) were synthesized by Kelowna International Scientific Inc. (Taipei, Taiwan). Polystyrenes (MW 770, 2430, 3700, 13,700, 18,700, 29,300, 44,000, 114,200 g/mol) were from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Poly(vinyl alcohol) (PVA, 88% hydrolyzed) was from Acros Organics Co. Inc. (Fair Lawn, NJ, USA). Phosphotungstic acid (PTA) was from Electron Microscopy Sciences (Hatfield, PA, USA). The bEnd.3 and U87-MG cell lines were from Bioresource Collection and Research Center (Hsinchu, Taiwan). Dulbecco’s Modified Eagle Medium (DMEM) powder was from Thermo Fisher Scientific Inc. (Grand Island, NY, USA).
Lo Y.C, & Lin W.J. (2021). Benefit of a Short Chain Peptide as a Targeting Ligand of Nanocarriers for a Brain-Driven Purpose. Pharmaceutics, 13(8), 1249.
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6

Synthesis of Bioactive PEG Hydrogel Precursors

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8-arm PEG-OH (20 kDa) and 4-arm PEG-amido succinic acid (PEG-ASA, 10 kDa) were purchased from JenKem Technology and Laysan Bio, respectively. 5-norbornene-2-carboxylic acid, N,N’-dicyclohexylcarbodiimide (DCC), 4-(dimethylamino)pyridine (DMAP), N,N-dimethylformamide (DMF), and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) were purchased from Sigma-Aldrich. Tetrazine-amine (Tz-NH2) and methyltetrazine amine (mTz-NH2) were obtained from Click Chemistry Tools. N,N’-diisopropylethylamine (DIEA) and 2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) were purchased from TCI and Anaspec, respectively. Heparin sodium salt was purchased from Celsus Laboratories and hyaluronic acid was obtained from Lifecore Biomedical. 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Acros and TCI, respectively. All other chemicals were purchased from Fisher Scientific unless noted otherwise. All peptides (>90% purity) were purchased from GenScript.
Arkenberg M.R., Dimmitt N.H., Johnson H.C., Koehler K.R, & Lin C.C. (2020). Dynamic click hydrogels for xeno-free culture of induced pluripotent stem cells. Advanced biosystems, 4(11), e2000129.
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7

Synthesis of High Molecular Weight γ-PGA Hydrogel

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High molecular weight poly(γ-glutamic acid) (γ-PGA, Mw 1,000,000 Da) was obtained from Vedan Corp (Taiwan). Dithiothreitol (DTT) was purchased from Fisher Scientific. 4-arm-PEG-SH (PEG4SH) was purchased from Laysan Bio, Inc. Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) was purchased from Sigma Aldrich. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Acros Organics and N-Hydroxysuccinimide (NHS) and 5-norbornene-2-methylamine were purchased from Tokyo Chemical Industry Co., Ltd. All reagents were used as received without further purification.
Seebach B.S., Intrator N., Lieberman P, & Cooper L.N. (1994). A model of prenatal acquisition of speech parameters.. Proceedings of the National Academy of Sciences of the United States of America, 91(16), 7473-7476.
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8

Graphene Oxide-Based Drug Delivery System

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Graphene oxide (N002-PDE) powder was obtained from Angstron Materials Inc. (Dayton, OH, USA). Potassium bromide (KBr) was purchased from Showa Chemical Co. (Tokyo, Japan). 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from Acros Organics (Geel, Belgium). Avidin and cetuximab (CET) was purchased from Calbiochem (San Diego, CA, USA) and Merck (Darmstadt, Germany), respectively. Biotin-PEG-NHS (molecular weight = 3400 Da) was purchased from Nanocs Inc. (New York, NY, USA). Doxorubicin (DOX), 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI), and RPMI-1640 medium for cell culture were all purchased from Sigma (St Louis, MO, USA). Live/Dead viability/cytotoxicity kit was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) purchased from Hyclone, GE Healthcare (Logan, UT, USA) was used for cell culture. All of the reagents were of analytical grade.
Lu Y.J., Lin P.Y., Huang P.H., Kuo C.Y., Shalumon K.T., Chen M.Y, & Chen J.P. (2018). Magnetic Graphene Oxide for Dual Targeted Delivery of Doxorubicin and Photothermal Therapy. Nanomaterials, 8(4), 193.
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9

Retinol-Based Nanoparticle Synthesis

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β-CD (97%), oleic acid, and DMSO were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All-trans-retinol and stearic acid were purchased from Sigma-Aldrich Chemicals Co. (Sigma-Aldrich, St. Louis, MO, USA). NHS was obtained from Fluka (Fluka Chemie AG, Buchs, Switzerland). 1-(3-Dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC) was purchased from Acros Organics (Morris, NJ, USA). Organic solvents, such as chloroform, hexane, ethyl acetate, acetone, and n-propyl alcohol, were of analytical grade, and the high-purity (Milli-Q, Darmstadt, Germany) water was used through the study.
Kim H., Hu Y., Jeong D., Jun B.H., Cho E, & Jung S. (2016). Synthesis, Characterization, and Retinol Stabilization of Fatty Amide-β-cyclodextrin Conjugates. Molecules, 21(7), 963.
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10

Stearic Acid Activation and Conjugation

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To prepare this formulation, activation of the carboxylic
acid groups of stearic acid was needed. It was achieved as described
in our previous study.39 (link) We dissolved 284
mg of stearic acid (MW: 284.48, Acros Organics, Geel, Belgium), 206
mg of 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride
(EDC; Acros Organics, Geel, Belgium), 140 mg of N-hydroxysuccinimide (NHS; Acros Organics, Geel, Belgium), and 0.250
mL of triethanol amine (TEA; Molekula GmbH, Munich, Germany) in 20
mL of DMSO in a beaker. We stirred the mixture for 2 h at 80 °C
and another 24 h at RT. In addition, we slowly added the activated
solution containing stearic acid to the CSFI solution, stirred for
10 h at 60 °C, and kept it at RT for another 24 h. The resulting
solution (CSFISA) was stored at −20 °C until further use.
AbouAitah K., Abdelaziz A.M., Higazy I.M., Swiderska-Sroda A., Hassan A.M., Shaker O.G., Szałaj U., Stobinski L., Malolepszy A, & Lojkowski W. (2024). Functionalized Carbon Nanotubes for Delivery of Ferulic Acid and Diosgenin Anticancer Natural Agents. ACS Applied Bio Materials, 7(2), 791-811.
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