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Bovine pancreas insulin

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Bovine pancreas insulin is a laboratory product derived from the pancreas of cows. It serves as a source of insulin, a hormone that regulates blood sugar levels. The product is used for research and analytical purposes in various scientific and medical applications.

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Lab products found in correlation

β glycerophosphate disodium salt hydrate, by Merck Group (1 mentions) 2 phospho l ascorbic acid trisodium salt, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions) Alizarin red s, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Low endotoxin bovine serum albumin, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Corticosterone, by Tokyo Chemical Industry (1 mentions) Epidermal growth factor (egf), by Fujifilm (1 mentions) Multiscan fc microplate reader, by Thermo Fisher Scientific (1 mentions) Riboflavin, by Merck Group (1 mentions) Auranofin, by Merck Group (1 mentions) Imidazole, by Merck Group (1 mentions) Chloramphenicol, by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Euroclone (1 mentions) 1 4 dithiothreitol, by Merck Group (1 mentions) Ampicillin, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions) L cysteine, by Merck Group (1 mentions)

11 protocols using bovine pancreas insulin

1

Multipotent MSC Differentiation into Adipocytes and Osteocytes

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The multipotent MSC D1 cell line was differentiated into adipocytes and osteocytes. For that purpose, the medium was exchanged immediately after labelling and replaced with fresh medium containing adipogenic or osteogenic supplementation. Adipogenic supplementation consisted of 100 nM Dexamethasone, 10 mM β-glycerophosphate disodium salt hydrate and 77 µM 2-Phospho-L-ascorbic acid trisodium salt (all from Sigma), whereas the osteogenic supplementation consisted of 100 nM Dexamethasone, 155 µM 2-Phospho-L-ascorbic acid trisodium salt, 50 µM indomethacin and 175 nM bovine pancreas insulin (all from Sigma). The cells were then allowed to grow for a further 9 d with periodic medium changes. Effective differentiation was evaluated by fixation and histochemical staining with 0.5% Oil Red O (Sigma) in isopropanol or 2% Alizarin Red S (Sigma).
Taylor A., Herrmann A., Moss D., Sée V., Davies K., Williams S.R, & Murray P. (2014). Assessing the Efficacy of Nano- and Micro-Sized Magnetic Particles as Contrast Agents for MRI Cell Tracking. PLoS ONE, 9(6), e100259.
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2

Glioblastoma Stem Cell Culture and Stress Induction

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Brain tumor–derived cultures were isolated from biopsies and established as previously described [28] (link). GSC-ECLs were cultured in serum-free medium consisting of neurobasal medium supplemented with B27, N2, 20 ng/ml basic fibroblast growth factor (bFGF), 20 ng/ml epidermal growth factor (EGF), 2 mM L-glutamine, 2 mM nonessential amino acids, 50 U/ml penicillin/streptomycin (all from Invitrogen, Carlsbad, CA), 20 μg/ml bovine pancreas insulin, and 75 μg/ml low-endotoxin bovine serum albumin (Sigma, St. Louis, MO) and plated onto Geltrex-coated plates (10 μg/ml) (Invitrogen, Carlsbad, CA). Cells were routinely grown to confluence, dissociated using Accutase, and then split 1:2 to 1:3. Medium was replaced every 2 to 3 days. To induce differentiation, cells were cultured for 14 days in the same medium without bFGF and EGF.
To induce genotoxic stress, cells were incubated in 1 μM CPT (Sigma, St. Louis, MO). To generate nutritional stress, cells were incubated in neurobasal medium without supplements.
Morris-Hanon O., Furmento V.A., Rodríguez-Varela M.S., Mucci S., Fernandez-Espinosa D.D., Romorini L., Sevlever G.E., Scassa M.E, & Videla-Richardson G.A. (2017). The Cell Cycle Inhibitors p21Cip1 and p27Kip1 Control Proliferation but Enhance DNA Damage Resistance of Glioma Stem Cells. Neoplasia (New York, N.Y.), 19(7), 519-529.
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3

Epidermal Growth Factor Signaling Protocol

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Epidermal growth factor (EGF) (human, recombinant, animal-derived-free) was purchased from FUJIFILM Wako (Osaka, Japan), fatty acid-free bovine serum albumin (BSA) from
Calbiochem-Novabiochem (San Diego, CA, USA), bovine pancreas insulin from Sigma-Aldrich (Tokyo, Japan), human GRF from the Peptide Institute (Osaka, Japan), and corticosterone from Tokyo
Chemical Industry (Tokyo, Japan). GPR4 antagonists were kindly provided by Dr S Shuto [14 (link)].
MUSHA S., YOSHIDA S., MURAKAMI S., KOJIMA R., DEAI M., SASO N., MOGI C., SATO K., OKAJIMA F, & TOMURA H. (2020). Involvement of GPR4 in increased growth hormone and prolactin expressions by extracellular acidification in MtT/S cells. The Journal of Reproduction and Development, 66(2), 175-180.
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4

Insulin Reduction Assay for PDI Activity

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This assay is based on the measurement of the catalytic reduction of insulin as described by Lundstrom and Holmgren [39 ]. PDI facilitates the reduction of insulin in the presence of Dithiothreitol (DTT). The reduced insulin chains aggregate and the turbidity is monitored spectrophotometrically at 650 nm. The assay was performed in a 96-well plate format and a volume of 100 μl in the presence of 1 mM DTT, 1 μg PDI (Sigma), 0.15 mM bovine pancreas insulin (Sigma), and 0.2 mM EDTA in 100 mM potassium phosphate, pH 7.0. The progress of the reaction was monitored on a 96-well plate reader (Multiscan FC Microplate Reader, Fisher Scientific) at 650 nm for 60 min at 25 °C. The non-enzymatic reduction of insulin by DTT was recorded in control well without PDI.
Inostroza-Nieves Y., Valentin-Berrios S., Vega C., Prado G.N., Luciano-Montalvo C., Romero J.R, & Rivera A. (2022). Inhibitory effects of Syzygium jambos extract on biomarkers of endothelial cell activation. BMC Complementary Medicine and Therapies, 22, 101.
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5

Recombinant protein purification protocol

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Isopropyl β-d-1-thiogalactopyranoside (IPTG), sodium selenite (Na2SeO3), l-cysteine, ampicillin, chloramphenicol, riboflavin, lysozyme, Tris(hydroxymethyl) aminomethane (Tris), 1,4-Dithiothreitol (DTT), β-mercaptoethanol (βme), potassium phosphate, sodium chloride (NaCl), imidazole, flavin adenine dinucleotide (FAD), Auranofin were from Sigma-Aldrich. 2-methyl-2,4-pentandiol v/v 100% (MPD) from Molecular Dimension. EDTA was from Euroclone. Phenylmethanesulfonylfluoride (PMSF) was from ICN Biomedicals Inc. NADPH, Tetrasodium salt was from Roche and/or Sigma. 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) and bovine pancreas insulin were obtained from Sigma. TRi-1 and TRi-2 were obtained from Oblique Therapeutics.
Fata F., Gencheva R., Cheng Q., Lullo R., Ardini M., Silvestri I., Gabriele F., Ippoliti R., Bulman C.A., Sakanari J.A., Williams D.L., Arnér E.S, & Angelucci F. (2022). Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus. Redox Biology, 51, 102278.
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6

Insulin Absorption and Lactoferrin Measurement

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Bovine pancreas insulin, FITC-Dextrans, Lactoferrin, and Krebs-Henseleit buffer were purchased from Sigma-Aldrich. Aimstrip Plus blood glucose strips, blood glucose monitor, and suture thread were obtained from VWR. Lactoferrin ELISA kits were purchased from Abcam. qRT-PCR reagents and kits were purchased from LifeTechnologies.
Gleeson J.P., Fein K.C., Chaudhary N., Doerfler R., Newby A.N, & Whitehead K.A. (2020). The enhanced intestinal permeability of infant mice enables oral protein and macromolecular absorption without delivery technology. International journal of pharmaceutics, 593, 120120.
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7

Fabrication of Microneedle Array Delivery System

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Soybean lecithin, propylene glycol (Tianjin Chemical Reagent Co., Ltd., China), and cetyltrimethyl ammonium bromide (Shanghai Aobo Chemical Reagent Co., Ltd., China) were purchased for the preparation of the nanovesicles. MA female molds (Micropoint Technologies Pte Ltd., Singapore) were purchased for the fabrication of the MA using a micromolding method. FITC, STZ and bovine pancreas insulin were purchased from Sigma-Aldrich (USA).
A New Zealand rabbit (male, 2–3 months, 3.0 kg) was offered by the Xinhua Experimental Animal Farm (Huadu district, Guangzhou). The rabbit was euthanized for hair removal. The rabbit skin was cut to an appropriate size. SD rats (male, 200 ± 30 g) were provided and quarantined from the Experimental Animal Center of Sun Yat-Sen University.
Yang J., Li Y., Ye R., Zheng Y., Li X., Chen Y., Xie X, & Jiang L. (2020). Smartphone-powered iontophoresis-microneedle array patch for controlled transdermal delivery. Microsystems & Nanoengineering, 6, 112.
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8

DMEM-based Cell Culture Protocol

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Dulbecco’s modified eagle’s medium (DMEM) was obtained from Life Technologies (Carlsbad, CA, USA). Dexamethasone (DEX), 3-isobutyl-1-methyl-xanthine (IBMX) and insulin (bovine pancreas) were purchased from Sigma (St. Louis, MO, USA).
Tsuji T., Ikado K., Koizumi H., Uchiyama S, & Kajimoto K. (2017). Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli. Scientific Reports, 7, 12889.
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9

Characterization of IFITM1 Regulation

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Ham’s F-12 (1×) nutrient mixture (catalogue number 11765-054), RPMI 1640 medium (catalogue number 11875-093), fetal bovine serum (FBS; catalogue number 16000-044), antibiotic/antimycotic solution (containing 10,000 U/ml penicillin, 10 mg/ml streptomycin, and 25 μg/ml Fungizone®), minimum essential medium nonessential amino acids, l-glutamine, and TrypLE (containing trypsin and ethylenediaminetetraacetic acid) were obtained from Life Technologies (Grand Island, NY, USA). Insulin (bovine pancreas), anti-β-actin, and hydrocortisone were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-IFITM1, anti-STAT1, anti-STAT2, anti-BRG1, anti-p-STAT2 (Tyr690), anti-interferon regulatory factor (IRF)-7, anti-IFNα, anti-p21, anti-cyclin D1, and anti-cyclin E antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and rabbit polyclonal and mouse monoclonal secondary antibodies and anti-p-STAT1 (Tyr701) were purchased from Cell Signaling Technology (Danvers, MA, USA). IFITM1 promoter constructs were kindly provided by Dr. Yeon-Su Lee from the Cancer Genomics Branch, National Cancer Center, Goyang-si, South Korea.
Ogony J., Choi H.J., Lui A., Cristofanilli M, & Lewis-Wambi J. (2016). Interferon-induced transmembrane protein 1 (IFITM1) overexpression enhances the aggressive phenotype of SUM149 inflammatory breast cancer cells in a signal transducer and activator of transcription 2 (STAT2)-dependent manner. Breast Cancer Research : BCR, 18, 25.
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10

Insulin-stimulated Glucose Uptake in Blastocysts

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Nonradioactive insulin-stimulated 2-deoxyglucose uptake into single blastocysts was performed using microfluorometric assays combined with enzymatic cycling reactions as previously described (Chi, et al., 2002 (link)). Briefly, two separate types of experiments were conducted. First, C57Bl/6 blastocysts or TallyHO blastocyst were isolated. Alternatively, C57Bl/6 or TallyHO embryos were collected at morula stage and cultured for 24 h in HTF media with added 25µg/ml Metformin. Next, blastocysts from each condition were incubated in HTF medium with or without a final glucose concentration of 5.6mmol/l with 500nmol/l insulin (bovine pancreas; Sigma, St. Louis, MO USA) for 30 min. 2-Deoxyglucose uptake was then measured as described previously (Chi, Hoehn and Moley, 2002 (link)) and expressed as millimoles per kilogram wet weight over a 15-min time interval. The rate is expressed as the difference between insulin-stimulated and basal values. This assay was conducted at least 3 times with each set of experiments. Fifteen to 20 blastocysts were used in each assay.
Louden E.D., Luzzo K.M., Jimenez P.T., Chi T., Chi M, & Moley K.H. (2014). TallyHO obese female mice experience poor reproductive outcomes and abnormal blastocyst metabolism which is reversed by metformin. Reproduction, fertility, and development, 27(1), 31-39.
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