The normal lung cell line LL24 and lung squamous cell line HCC-1588 were obtained from the Korean Cell Line Bank (Seoul, Korea). The human embryonic kidney cell line HEK293 and lung adenocarcinoma cell line A549 were obtained from ATCC. HEK293 cells were selected for higher transfection efficiency. LL24, A549, and HCC-1588 cells were grown in RPMI-1640 medium (Gibco, Life Technologies, Grand Island, NY) with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2. HEK293 cells were grown in Dulbecco’s modified Eagle’s medium (Gibco, Life Technologies, Grand Island, NY) with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2. These cell lines were authenticated by monitoring of cell morphology and by contamination inspection.
Hcc1588
Manufactured by Korean Cell Line Bank
Sourced in United States
The HCC1588 is a laboratory equipment product designed for cell culture and research applications. It is a specialized incubator that provides a controlled environment for the growth and maintenance of cell lines. The HCC1588 regulates temperature, humidity, and CO2 levels to ensure optimal conditions for cell culture. It is suitable for a range of cell types and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Hcc95, by Korean Cell Line Bank (3 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Hek293, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Bronchial epithelial cell growth medium bullet kit, by Lonza (1 mentions)
Hcc2108, by Korean Cell Line Bank (1 mentions)
Hcc827, by Korean Cell Line Bank (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Sk mes 1, by Korean Cell Line Bank (1 mentions)
Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Mk 1775, by Selleck Chemicals (1 mentions)
Doxycycline d9891, by Merck Group (1 mentions)
Matrigel, by Corning (1 mentions)
Calu 6, by American Type Culture Collection (1 mentions)
Nci h23, by American Type Culture Collection (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
4 protocols using hcc1588
1
Cell Line Characterization and Culture
2
Culturing NSCLC and Lung Epithelial Cells
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Healthy BEAS-2B human epithelial lung and H1703 human squamous carcinoma cells (American Type Culture Collection, Manassas, VA, USA), and A549, HCC827, HCC2108, HCC95, HCC1588, and HCC1195 NSCLC cells (Korean Cell Line Bank, Seoul, Korea) were cultured as follows. BEAS-2B cells were cultured using the Bronchial Epithelial Cell Growth Medium Bullet Kit (Lonza Group Ltd., Basel, Switzerland). NSCLC cells were maintained in RPMI-1640 (WELGENE Inc., Gyeongsan-si, Korea) supplemented with 10% heat-inactivated fetal bovine serum, glutamine (2 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL).
3
Lung SCC Cell Lines Knockdown of MIF
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Five human lung SCC cell lines, HCC‐95, HCC‐1588, SNU‐1300, SW‐900 and SK‐MES‐1, were purchased from the Korean cell line bank (Seoul, South Korea). The cell lines were cultured in RPMI‐1640 medium (Gibco, 22400‐089) supplemented with 10% fetal bovine serum (FBS, Gibco, 26140‐079), 1% penicillin‐streptomycin (Corning, 30‐002‐CI) at 37°C in 5% CO2.
To achieve knockdown of MIF, HCC‐1588 cells were transfected with small interfering RNA (siRNA). Cells were cultured in Opti‐MEM reduced serum medium (Gibco) in accordance with the manufacturer's instructions. All siRNAs were transfected using lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. Cells were harvested 72 hours after transfection and the levels of MIF mRNA were assessed by semi‐quantitative polymerase chain reaction (PCR). Western blot analysis was used to measure MIF.
To achieve knockdown of MIF, HCC‐1588 cells were transfected with small interfering RNA (siRNA). Cells were cultured in Opti‐MEM reduced serum medium (Gibco) in accordance with the manufacturer's instructions. All siRNAs were transfected using lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. Cells were harvested 72 hours after transfection and the levels of MIF mRNA were assessed by semi‐quantitative polymerase chain reaction (PCR). Western blot analysis was used to measure MIF.
4
Lung Cancer Cell Lines Culture Protocol
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A549, Calu-6, NCI-H1299, NCI-H1703, NCI-H1975, NCI-H23, NCI-H460, HCC2279, NCI-H522, A427, Calu-3, NCI-H358, HCC827 and BEAS2B were obtained from the American Type Culture Collection (Rockville, MD, USA). HCC95 and HCC1588 were obtained from the Korean Cell Line Bank (Seoul, Korea), and Normal human bronchial epithelial (NHBE) purchased from Lonza. A549, Calu-6, NCI-H1299, NCI-H1703, NCI-H1975, NCI-H23, NCI-H460, HCC2279, NCI-H522, NCI-H358, HCC827, HCC95, HCC1588, and BEAS2B were cultured with RPMI1640. Calu-3 and A427 were cultured with Dulbecco’s modified eagle medium (DMEM). NHBE was cultured with bronchial epithelial cell growth medium (BEGM) BulletKit (Lonza, Walkersville, MD, USA). All cell lines were maintained in media supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin, and cultured under standard conditions at 37 °C in a humidified atmosphere of 95% air and 5% CO2. Stock solutions of the Wee1 inhibitor MK-1775 (Selleck Chemicals, Houston, TX, USA) were dissolved in dimethyl sulfoxide (DMSO) and added to the media at the indicated concentrations (100 nM). Doxycycline (D9891) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Matrigel was purchased from Corning Inc (Corning, NY, USA).
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