Direct q water purification system

BPA and BPS were obtained from Sigma-Aldrich (St. Luis, MO, USA). HPLC grade acetonitrile and ethanol were purchased from S. Witko - JT Baker (Lodz, Poland). Water used as a HPLC solvent was purified with a Direct-Q Water Purification System (Merck). All other chemicals were of analytical grade and purchased from Merck.
Water and sediments samples were collected from Rawa River in South Poland (50° 15′ 36″ N 19° 01′ 41″ E). The river is a sewage receiver from the wastewater treatment plant Klimzowiec in Chorzów, Poland. The water sample was collected under 10 cm of the water surface, while the sediment samples were taken from depths 0 to 10 cm. The samples were packed into sterile glass jars and immediately transferred to the laboratory and used to prepare microcosms.

CHX (99.5%) was obtained from Merck (Darmstadt, Germany). OCT dihydrochloride (98%) was obtained from Alfa Aesar (Ward Hill, MA, USA). The tested compounds were diluted in DMSO (99.9%, Merck). Malt extract broth (Oxoid, Basingstoke, UK) and glucose (Penta, Prague, Czech Republic) were used for the MEG medium for fungal cultivation. ACN (HPLC grade and LC-MS grade, VWR, Prague, Czech Republic), milli-Q water prepared by the Direct-Q^® water purification system (18.2 MΩ·cm, Merck), FA (98%, Penta, Czech Republic), FA (LC-MS grade, Honeywell, Charlotte, NC, USA), and trifluoroacetic acid (TFA ≥ 99%, Merck) were used for quantitative analyses.

Bisphenol A and ethyl acetate (HPLC grade) were obtained from Merck (Darmstadt, Germany). HPLC grade acetonitrile and ethanol were purchased from S. Witko - JT Baker (Lodz, Poland). Water used as a HPLC solvent was purified with a Direct-Q® Water Purification System (Merck). The standard stock solutions of BPA (7 g L^− 1 or 5 mg 5 mL^− 1) were prepared in 70% ethanol and stored at 4 °C up to three months. Final concentration of BPA and ethanol in medium were 100 mg L^− 1 and 1% (v/v), respectively. Basal Salt Medium (BSM) (Badiefar et al. 2015 (link)) was used for isolation and purification of bacterial species and the degradation study. For bacteria isolation, BSM was supplemented with nystatin (4 g L^− 1) and actidione (4 g L^− 1) to inhibit the growth of fungi.

Rosmarinic acid and the 7-O-glucoside derivatives of apigenin, luteolin, eriodyctiol, quinic acid, caffeic acid, 5-O-caffeoylquinic acid, 4-O-hydroxybenzoic acid, salvianolic acid B, caffeic acid, ferulic acid, and 4-O-coumaric acid were obtained from Extrasynthese (Genay Cedex, France). Trolox, sulforhodamine B (SRB), DPPH (2,2-diphenyl-1-picrylhydrazyl) radical, acetic acid, ellipticine, trypan blue, trichloroacetic acid (TCA), Tris, lipopolysaccharide (LPS), carnosol, nisin, ascorbic acid, and butylated hydroxyanisole (BHA) were obtained from Sigma Chemical Co (St Louis, MO, USA). Fetal bovine serum (FBS), L-glutamine, trypsin-ethylenediaminetetraacetic acid (EDTA), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), Roswell Park Memorial Institute (RPMI) 1640 Medium and Dulbecco’s Modified Eagle Medium (DMEM) were from Hyclone (Logan, Utah, UT, USA). The Griess reagent system was purchased from Promega Corporation (Madison, WI, USA). Mueller-Hinton agar was from VWR (Prolabo Chemicals, USA); formic acid and ethanol was from Panreac (Barcelona, Spain). n-Hexane, methanol, and acetonitrile were purchased from Lab-Scan (Lisbon, Portugal). Purified water was obtained from a Direct-Q^® water purification system (Merck Life Science, Darmstadt, Germany).

P-cresol (Acros Organics, NJ, 99+%) was dissolved in methanol. For the detection of tri-p-cresol phosphate (TCP, Pfaltrz & Bauer, CT) in solution, it was dissolved in methanol. Folin–Denis Reagent was purchased from Sigma-Aldrich (St. Louis, MO, USA). All D.I. water used was from a Millipore Direct-Q water purification system (EMD Millipore, Bedford, MA, USA, with a resistivity of 18 MΩcm⁻²).

Rosmarinic acid, apigenin-7-O-glucoside, luteolin-7-O-glucoside and eriodictyol-7-O-glucoside were obtained from Extrasynthese (Genay, France). Gallic acid, nisin, ascorbic acid and 2,6-Di-tert-butyl-4-methylphenol and the fatty acids methyl ester (FAME) reference standard mixture 37 (fatty acids C4 to C24; standard 47885-U) from Supelco (Bellefonte, PA, USA) were obtained from Sigma Chemical Co (St. Louis, MO, USA). Folin–Ciocalteu reagent, Na₂CO₃, formic acid and ethanol were purchased from Panreac (Barcelona, Spain). n-hexane, methanol, and acetonitrile with HPLC purity were purchased from Lab-Scan (Lisbon, Portugal). Mueller–Hinton agar was obtained from VWR, Prolabo Chemicals (Radnor, PA, USA). Water was treated in a Direct-Q ^® water purification system (Merck Life Science, Darmstadt, Germany).