H1703

Manufactured by Thermo Fisher Scientific

Sourced in United States

The H1703 is a laboratory centrifuge designed for general-purpose applications. It is capable of reaching a maximum speed of 6,000 RPM and has a maximum capacity of 4 x 250 mL. The centrifuge is constructed with a durable metal housing and features an easy-to-use control panel.

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NCI-H1703 (6 citations)

9 protocols using h1703

Cultivation of BEAS-2B and NSCLC Cell Lines

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The human nontumorigenic bronchial epithelial cell line BEAS-2B was purchased from the Shanghai Academy of Life Science (Shanghai, China) and cultured in the BEGM™ Bronchial Epithelial Cell Growth Medium (Lonza/Clonetics Corporation) supplemented with 0.5 ng/ml epidermal growth factor, 500 ng/ml hydrocortisone, 0.035 ng/ml bovine pituitary extract, 500 mM ethanolamine, 500 nM ethanolamine phosphate, 0.01 mg/ml adrenaline, and 0.1 g/ml retionic acid.
Five human NSCLC cell lines, namely, H522 (human lung adenocarcinoma cell carcinoma), H460 (human lung large cell carcinoma), H1703 (human lung squamous cell carcinoma), A549 (NSCLC), and SK-MES-1 (human lung squamous cell carcinoma), were also obtained from the Shanghai Academy of Life Science. The cell lines H522, H460, H1703, and A549 were maintained in RPMI-1640 media (Gibco/Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco/Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Gibco/Thermo Fisher Scientific, Inc.). SK-MES-1 cells were cultured in Minimal Essential Medium (Gibco/Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were cultured at 37°C in an incubator supplied with 5% CO₂.

Liu X., Huang S., Guan Y, & Zhang Q. (2020). Long noncoding RNA OSER1-AS1 promotes the malignant properties of non-small cell lung cancer by sponging microRNA-433-3p and thereby increasing Smad2 expression. Oncology Reports, 44(2), 599-610.

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Cell Line Culture Protocols for NSCLC

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Five NSCLC adenocarcinoma cell lines (PC9, SPC-A1, NCI-H1975, H1299, and A549), and three NSCLC squamous carcinomas cell lines (H520, H1703, and SK-MES-1) were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). A549, H1975, H1299, H1703 and H520 cells were cultured in RPMI 1640; 16HBE, SK-MES-1, PC9 and SPC-A1 cells were cultured in DMEM (GIBCO-BRL) medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C/5% CO₂.

Zang C., Nie F.Q., Wang Q., Sun M., Li W., He J., Zhang M, & Lu K.H. (2016). Long non-coding RNA LINC01133 represses KLF2, P21 and E-cadherin transcription through binding with EZH2, LSD1 in non small cell lung cancer. Oncotarget, 7(10), 11696-11707.

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Cell Lines for NSCLC Research

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Three human NSCLC adenocarcinoma cell lines (SPCA1, A549, and PC9), three NSCLC squamous carcinomas cell lines (H1703, SK-MES-1, and H226), and one normal human bronchial epithelial cell line (16HBE) were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). SPCA1, PC9, and H1703 were cultured in DMEM (GIBCO-BRL, NY, USA) medium, A549 and H226 were cultured in RPMI 1640 (GIBCO-BRL, NY, USA) medium, while SK-MES-1 was cultured in EMEM (GIBCO-BRL, NY, USA) medium. All cells were supplemented with 10% fetal bovine serum (FBS, AUS), 100 U/ml penicillin, and 100 mg/ml streptomycin and kept in an incubator at 37 °C with 5% CO₂.

Wang J., He X., Yao Q., Wang C., Lu X., Wang R, & Miao D. (2023). LncRNA PTTG3P promotes tumorigenesis and metastasis of NSCLC by binding with ILF3 to maintain mRNA stability and form a positive feedback loop with E2F1. International Journal of Biological Sciences, 19(13), 4291-4310.

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Cultivation of Human Lung Cancer Cell Lines

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Human LUSC cell lines SK-MES-1 (HTB-67), H1703 (CRL-2073), H226 (CRL-5826), H520 (HTB-182), and the normal human bronchial epithelial Beas-2B cell line (CRL-9609) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). SK-MES-1 and Beas-2B cells were cultured in DMEM (12100046, Gibco, USA) while H1703, H226, and H520 cell lines were cultivated in RPMI 1640 medium (61870127, Gibco, USA). All the cells were supplemented with 10% fetal bovine serum (FBS; 10270-106, Gubco, USA) and 100× penicillin/streptomycin solution (SV30010, PERFEMIKER, Shanghai, China) in a humid incubator with 5% CO₂ at 37°C.

Hu S., Cao P., Kong K., Han P., Yue J., Deng Y., Li F, & Zhao B. (2022). circCNN2 Accelerates Cell Proliferation and Invasion in Lung Squamous Cell Carcinoma via Regulating miR-184/E2F1 and Activating MAPK Signaling Pathway. Disease Markers, 2022, 6329097.

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Protocol for Establishing Murine Lung Carcinoma Models

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Female BALB/c nude mice (BALB/cAnNShjhnu) and C57BL/6 mice (C57BL/6JShjh) (6–8 weeks old) were purchased from Shanghai Jihui Laboratory Animal Care Co., Ltd (Shanghai, China) and maintained under SPF conditions in a controlled environment of 20–22 °C, with a 12/12 h light/dark cycle, 50–70% humidity, and food and water provided ad libitum. The small animal euthanasia equipment was used for animal euthanasia. Mice were put into the euthanasia chamber filling with 99.9% of CO₂ gas for 10 min. All animal experiments were performed under approval by the Shanghai Medical Experimental Animal Care Commission.
All of human NSCLC cell lines (A549, H1299, HCC827, H1975, H1703, H460, and H1650), human bronchial epithelial cell line (BEAS-2B), and murine lung carcinoma cell line (LLC1) were purchased from the American Type Culture Collection (ATCC). A549 cells were cultured in F-12K medium (Gibco) with 10% FBS (Gbico). H1299, HCC827, H1975, H1703, H460, and H1650 cells were cultured in RPMI-1640 medium (Gibco) with 10% FBS. BEAS-2B cells were cultured in BEGM medium (Gibco) with 10% FBS. LLC1 cells were cultured in DMEM medium (Gibco) with 10% FBS. They were all cultured at 37 °C with 5% CO₂.

Li B., Zhu L., Lu C., Wang C., Wang H., Jin H., Ma X., Cheng Z., Yu C., Wang S., Zuo Q., Zhou Y., Wang J., Yang C., Lv Y., Jiang L, & Qin W. (2021). circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity. Nature Communications, 12, 295.

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Characterization of NSCLC Cell Lines

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Human NSCLC cell lines H1975 (adenocarcinoma), H1703 (squamous carcinoma), and A549 (squamous carcinoma) were purchased from American Type Culture Collection (ATCC, Manassas, VA). ATCC characterizes each cell line using cytochrome C oxidase I (COI) and short tandem repeat (STR) testing. H1975 and H1703 were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA) and 1.0% penicillin-streptomycin at 37°C in an atmosphere of 5% CO₂. A549 was maintained in Roswell Park Memorial Institute medium (RPMI-1640, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA) and 1.0% penicillin-streptomycin at 37°C in an atmosphere of 5% CO₂.

Dorsey J.F., Kao G.D., MacArthur K.M., Ju M., Steinmetz D., Wileyto E.P., Simone CB I.I, & Hahn S.M. (2014). Tracking Viable Circulating Tumor Cells (CTCs) in the Peripheral Blood of Non-Small Cell Lung Cancer Patients Undergoing Definitive Radiation Therapy: Pilot Study Results. Cancer, 121(1), 139-149.

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Cell Line Culture Conditions

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The lung SqCC H1703, H2170, SK-MES-1, and H520 cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). The H1703, H2170, and H520 cell lines were maintained in Roswell Park Memorial Institute (RPMI)-1640 medium containing 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA), and SK-MES-1 cells were maintained in Dulbecco's modified Eagle's medium (DMEM)/F12 containing 10% FBS.

Che J., Yue D., Zhang B., Zhang H., Huo Y., Gao L., Zhen H., Yang Y, & Cao B. (2018). Claudin-3 Inhibits Lung Squamous Cell Carcinoma Cell Epithelial-mesenchymal Transition and Invasion via Suppression of the Wnt/β-catenin Signaling Pathway. International Journal of Medical Sciences, 15(4), 339-351.

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Comprehensive Cell Lines Culturing Protocol

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Human breast cancer cell lines MDA-MB-231 (HTB-26), BT549 (HTB-122), SK-BR-3 (HTB-30), lung cancer cell lines A549 (CCL-185), H1703 (CRL-5889), H1792 (CRL-5895) and H358 (CRL-5807), and embryonic kidney cell line HEK293 (CRL-1573) were obtained from American Type Culture Collection. Human breast cancer cell line SUM159 was a kind gift from Dr. Chenfang Dong, Zhejiang University. MDA-MB-231, BT549, H1703, H1792, and H358 cells were cultured in RPMI 1640 medium (Invitrogen), whereas SK-BR-3, SUM159, A549, and HEK293 cells were cultured in DMEM medium (Invitrogen), supplemented with 10% fetal bovine serum (Gibco) and incubated at 5% CO₂ incubator (Thermo) at 37°C and 95% humidity.
Cells were transfected with various plasmids using Lipofectamine 3000 Transfection Reagent or with si-RNA oligonucleotides by Lipofectamine RNAiMAX Transfection Reagent, according to the manufacturer’s instructions, respectively.

Zhou Q., Lin W., Wang C., Sun F., Ju S., Chen Q., Wang Y., Chen Y., Li H., Wang L., Hu Z., Jin H., Wang X, & Sun Y. (2022). Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. Nature Communications, 13, 3034.

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Culturing Lung Cancer Cell Lines

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Three cell lines (two lung adenocarcinoma cell lines, A549 and SPC-A1, and one NSCLC squamous carcinomas cell line, H1703) were purchased from the Institute of Biochemistry and Cell Biology, The Chinese Academy of Sciences (Shanghai, China). A549, H1703 cells were cultured in RPMI Medium 1640 basic media (GIBCO-BRL, Invitrogen, Carlsbad, CA), and SPCA1 was cultured in Dulbecco's modified Eagle's media (DMEM; GIBCO-BRL, Invitrogen) supplemented with heat-inactivated 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) (Invitrogen, Carlsbad), in a humidified incubator at 37°C with 5% CO₂.

Ma C., Wu G., Zhu Q., Liu H., Yao Y., Yuan D., Liu Y., Lv T, & Song Y. (2017). Long intergenic noncoding RNA 00673 promotes non-small-cell lung cancer metastasis by binding with EZH2 and causing epigenetic silencing of HOXA5. Oncotarget, 8(20), 32696-32705.

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