Tpl s eslide manger database

Manufactured by Leica

The TPL's eSlide Manager database is a software solution designed to manage digital slide images and associated data. It provides a centralized platform for storing, organizing, and accessing microscope slide images and related information.

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Lab products found in correlation

Aperio scanscope fl, by Leica (1 mentions) Aperio versa digital pathology scanner, by Leica (1 mentions) Bond rx autostainer, by Leica (1 mentions)

2 protocols using tpl s eslide manger database

Quantitative Analysis of EBI3 and IL12a Co-expression in Tumor and Lymphoid Tissues

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Slides containing fluorescently labeled tissue sections were scanned in the Aperio ScanScope FL (Leica Biosystems) using the 20X objective and images were archived in TPL’s eSlide Manger database (Leica Biosystems). Images were manually annotated to demarcate tumor (all images) and lymphoid regions (present on three images) using Aperio ImageScope (v12.4.2.700). To reduce operator bias, annotations in lymphoid regions were made using the Hoechst nuclear counterstain layer (EBI3 and IL12a image layers were not visible). Annotated images were then analyzed using Tissue Studio software (Tissue Studio version 2.7 with Tissue Studio Library version 4.4.2; Definiens Inc., Carlsbad CA). For analysis of tumors, regions positive for cytokeratin were digitally separated from the surrounding stroma (Tissue Studio Composer software) followed by cellular analysis for co-expression of EBI3, IL12a and cytokeratin. In lymphoid areas, cellular analysis for co-expression of EBI3 and IL12a was performed on all cells within the annotation regions. Analysis data included the percentage of co-expressing cells and the average cellular fluorescent intensities for each antibody marker.

Mirlekar B., Michaud D., Lee S.J., Kren N.P., Harris C., Greene K., Goldman E.C., Gupta G.P., Fields R.C., Hawkins W.G., DeNardo D.G., Rashid N.U., Yeh J.J., McRee A.J., Vincent B.G., Vignali D.A, & Pylayeva-Gupta Y. (2020). B cell–derived IL35 drives STAT3-dependent CD8+ T-cell exclusion in pancreatic cancer. Cancer immunology research, 8(3), 292-308.

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Visualizing KRAS RNA Expression

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KRAS RNA was detected using the RNAscope® 2.5 LS Probe for Hs-KRAS-O1 (ACD Biotechne cat. # 522968) in a Bond RX autostainer (Leica Biosystems) following the manufacturer’s directions. TSA-Cy5 and DAPI were used to visualize RNAScope signal and nuclei, respectively. Slides containing RNAscope fluorescently labeled cells were scanned either in the Aperio ScanScope FL or the Aperio Versa Digital Pathology Scanner using a 20X objective (Leica Biosystems). Images were archived in TPL’s eSlide Manger database (Leica Biosystems). Images were manually annotated for regions of interest using Tissue Studio software (Definiens Inc.Tissue Studio version 2.7 with Tissue Studio Library version 4.4.2). See supplementary methods for a detailed protocol.

Goulet D.R., Foster JP I.I., Zawistowski J.S., Bevill S.M., Noël M.P., Olivares-Quintero J.F., Sciaky N., Singh D., Santos C., Pattenden S.G., Davis I.J, & Johnson G.L. (2020). Discrete Adaptive Responses to MEK Inhibitor in Subpopulations of Triple-Negative Breast Cancer. Molecular cancer research : MCR, 18(11), 1685-1698.

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