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Prominence lc 20 ab hplc system

Manufactured by Waters Corporation
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The Prominence LC-20 AB HPLC system is a high-performance liquid chromatography (HPLC) instrument designed and manufactured by Waters Corporation. It is a modular system that includes a solvent delivery unit, an autosampler, a column oven, and a photodiode array (PDA) detector. The system is capable of performing a variety of HPLC separations and analyses.

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Lab products found in correlation

Resipore columns, by Agilent Technologies (3 mentions) 2410 refractive index detector, by Waters Corporation (3 mentions) Labsolutions software, by Shimadzu (1 mentions)

4 protocols using prominence lc 20 ab hplc system

1

Molecular Weight Analysis of Polymers

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After drying, retrieved samples were dissolved in tetrahydrofuran (10 mg/ml), filtered (0.45μm filter) and analyzed for the weight average molecular weight using two ResiPore columns (300 X 7.5 mm, 3 μm particle size; Agilent Technologies) in series in a Shimadzu Prominence LC-20 AB HPLC system with Waters 2410 refractive index detector. Polystyrene (Mw: 162 Da -364 kDa, Agilent, Santa Clara, CA) was used as standard and samples were injected at flow rate of 1mL/min at 40 °C [14 (link)].
Venkatesan N., Liyanage A.D., Castro-Núñez J., Asafo-Adjei T., Cunningham L.L., Dziubla T.D, & Puleo D.A. (2019). Biodegradable Polymerized Simvastatin Stimulates Bone Formation. Acta biomaterialia, 93, 192-199.
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2

Measuring Polymer Molecular Weight

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Molecular weight was measured using a Shimadzu Prominence LC-20 AB HPLC system with a Waters 2410 refractive index detector. Two 300 × 7.5 mm, 3 μm particle size ResiPore columns (Agilent Technologies) in series were used for sample separation. Samples were injected using THF as the eluent at a 1.0 ml/min flow rate. Standard curves were prepared using polystyrene standards ranging from 160 Da to 370 kDa. Shimadzu Lab Solutions software was used to calculate weight- (Mw) and number-average molecular weight (Mn) and the polydispersity index (PDI, Mw/Mn). Simvastatin monomer conversion (i.e., molecular weight growth of the poly(simvastatin) block) was determined as a function of time.
Asafo-Adjei T.A., Dziubla T.D, & Puleo D.A. (2014). Synthesis and Characterization of a Poly(ethylene glycol)-Poly(simvastatin) Diblock Copolymer. RSC advances, 4(102), 58287-58298.
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3

HPLC Polymer Molecular Weight Analysis

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A Shimadzu Prominence LC-20 AB HPLC system connected to a Waters 2410 refractive index detector was used to measure the number average (Mn) and weight average (Mw) molecular weights of polymeric prodrugs. Two Resipore columns in series (300 × 7.5 mm, 3 μm particle size; Agilent Technologies) were used for separation. Samples were dissolved in THF at 1 mg/mL and injected using THF as the mobile phase at a 1.0 mL/min flow rate at 40 ºC. The polystyrene standards used to calculate molecular weight ranged from 162 Da to 364 kDa.
Liyanage A.D., Chen A.J, & Puleo D.A. (2018). Biodegradable Simvastatin-Containing Polymeric Prodrugs with Improved Drug Release. ACS biomaterials science & engineering, 4(12), 4193-4199.
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4

Molecular Weight Determination of Polymers

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GPC was used to determine the molecular weight of the synthesized polymers in a Shimadzu Prominence LC-20 AB HPLC system installed with a Waters 2410 refractive index detector. A Polargel-M 300x7.5 mm 8μm column (Agilent) was used for separation. The linear range of calibration for molecular weight determination was from 1,840 DA to 2,210,000 DA of polymethylmethacrylate (PMMA) standards (Agilent). A set of polymeric beads from Group 1 were dissolved in DMSO at 5-7 mg/ml and passed through the GPC for determination of the molecular weight. Five replicates were completed for each polymeric system and the values are reported with their respective standard deviation.
Shah R.A., Runge T., Ostertag T.W., Tang S., Dziubla T.D, & Hilt J.Z. (2021). Development of temperature-responsive polymeric gels with physical crosslinking due to intermolecular – interactions. Polymer international, 71(3), 292-300.
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