Orbitrap xl ms

Manufactured by Thermo Fisher Scientific

The Orbitrap XL MS is a high-resolution mass spectrometer produced by Thermo Fisher Scientific. It utilizes Orbitrap technology to provide accurate mass measurements and high-resolution data for a variety of analytical applications.

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Lab products found in correlation

Nicolet 6700 ft ir, by Thermo Fisher Scientific (3 mentions) Hypersil gold c18 column, by Thermo Fisher Scientific (1 mentions) Accela pda detector, by Thermo Fisher Scientific (1 mentions) Thermal analyzer, by TA Instruments (1 mentions) Inova 400 or500 mhz spectrometer, by Agilent Technologies (1 mentions)

3 protocols using orbitrap xl ms

Kinetics of FVIII Amide Hydrogen-Deuterium Exchange

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Local amide hydrogen/deuterium exchange (HDX) kinetics were followed after 3, 10 and 30 s; 2, 10 and 60 min; 3 h; and 3 days of incubation. All HDX reactions were performed at 22°C, except for the 3-s reaction (6°C). Human rFVIII containing 70% B-domain (B70-rFVIII) was mixed with deuterated buffer (Tris, pH 6.7, containing CaCl₂ and NaCl). The reaction was stopped with ice-cold phosphate buffer, pH 2.3, containing 100 mM Tris(2-carboxyethyl)phosphine and 3.3 M urea, and by subsequent snap freezing in liquid nitrogen. Samples were digested using a high-performance liquid chromatography (HPLC) column (ACE, Aberdeen, UK) packed with pepsin-agarose from porcine gastric mucosa (Sigma-Aldrich) and desalted on a C18 pre-column (ACE). Peptic peptides were subjected to liquid chromatography coupled to mass spectrometry (MS) using a HALO C18/1.8 μm column (Advanced Materials Technology, Wilmington, DE, USA). Peptides were eluted by an acetonitrile gradient and analysed on an Orbitrap XL MS (60,000 resolution at m/z 400; Thermo Fisher Scientific). Peptic peptides were identified by three independent liquid chromatography-MS/MS analyses of a non-deuterated sample using the same procedure as for the deuterated samples.

Anzengruber J., Feichtinger M., Bärnthaler P., Haider N., Ilas J., Pruckner N., Benamara K., Scheiflinger F., Reipert B.M, & Malisauskas M. (2019). How Full-Length FVIII Benefits from Its Heterogeneity – Insights into the Role of the B-Domain. Pharmaceutical Research, 36(5), 77.

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UHPLC-HRMSn Characterization of Compounds

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The UHPLC-HRMSn system consists of a LTQ Orbitrap XL MS with an Accela 1250 binary Pump, a PAL HTC Accela TMO autosampler, an Accela PDA detector (Thermo Fisher Scientific, San Jose, CA), and an Agilent G1316A column compartment (Agilent, Santa Clara, CA). The operating conditions of the electrospray (ESI) source were similar to our previous work [25 (link)] with the differeces were as follows: sheath gas at 70 (arbitrary units), spray voltage at 4.5 kV; capillary temperature, 250 °C; capillary voltage, 50 V; tube lens offset, 120 V. MSⁿ activation parameters used an isolation width of 2.0 amu, max ion injection time of 200 ms, and normalized collision energy at 35%. The UHPLC separation was carried out on a Hypersil Gold C₁₈ column (200 mm × 2.1 mm, 1.9 μm) (Thermo Fisher Scientific, San Jose, CA) with a flow rate of 0.3 mL/min. The mobile phase consisted of a combination of A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile). A gradient elution program was employed as follows: from 5% to 25% B over 0–30.0 min, to 30% B at 40.0min, to 85% B at 60.0 min, to 95 % B at 65.0 min, and then a return to the initial conditions. The column temperature was set at 50 °C.

Geng P., Harnly J.M., Sun J., Zhang M, & Chen P. (2017). Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of Black Cohosh (Actaea racemosa) – an UHPLC-HRAM-MS Chemometrics Study. Analytical and bioanalytical chemistry, 409(10), 2591-2600.

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Characterization of Organic Compounds

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NMR spectra were collected on a Varian Inova 400 or 500 MHz spectrometer using residual proton solvent signals as an internal reference. Mass spectrometry data were collected by one of two methods. 3a, 3b, 8a, and 8b were analyzed by direct infusion into a Synap HDMS quadrupole TOF-MS (Waters, Beverly, MA) with a resolving power of 9000. The remainder of the positive ion mass spectra were recorded by direct infusion into an Orbitrap XL MS (Thermo-Fisher Scientific, Waltham, MA) at a resolving power of 30 000. Differential scanning calorimetry traces were recorded on a TGA Q10 DSC system with a TA Instruments thermal analyzer at a heating rate of 10 °C min⁻¹. Elemental analyses were collected by Robertson Microlit Laboratories, Inc. IR spectra were recorded via the dropcast method from methanolic solution onto a KBr plate with a Thermo Nicolet 6700 FT-IR. For each spectrum, the resolution was 4 cm⁻¹ and 256 scans were collected. X-ray crystallographic data were collected by the Indiana University Molecular Structure Center on a Bruker APEX II Kappa Duo diffractometer using Mo Kα radiation (graphite monochromator) at 150 K. Details of the X-ray analysis can be found in the Supporting Information.

Kirschner K.M., Ratvasky S.C., Pink M, & Zaleski J.M. (2019). Anion Control of Lanthanoenediyne Cyclization. Inorganic chemistry, 58(14), 9225-9235.

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