Wl00009b

Manufactured by Wanlei

Sourced in China

The WL00009b is a lab equipment product manufactured by Wanlei. It is a precision measurement device designed for use in scientific and research applications. The core function of the WL00009b is to provide accurate and reliable measurements, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated goat anti rabbit igg zb 2301, by ZSGB-BIO (2 mentions) Kgp2100, by Keygen Biotech (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) Protein assay kit, by Boster Bio (1 mentions) Ab53066, by Abcam (1 mentions) Ab7213, by Abcam (1 mentions) Ab89887, by Abcam (1 mentions) Sc 398182, by Santa Cruz Biotechnology (1 mentions) Vegfa, by Wanlei (1 mentions) Ab5694, by Abcam (1 mentions) β actin, by ZSGB-BIO (1 mentions) P iκbα, by Cell Signaling Technology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) β actin, by Boster Bio (1 mentions) Sc 504, by Santa Cruz Biotechnology (1 mentions)

5 protocols using wl00009b

Quantitative Analysis of VEGFA Levels

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HUVECs with or without SCF were
lysed with RIPA lysate (Pro Tech) at hours 3, 6, and 9. Protein concentrations
were tested with a protein assay kit (Bosterbio). The total proteins
were separated by SDS-PAGE, transferred to a PVDF membrane (the membrane
was blocked with 5% non-fat milk), incubated with a vascular endothelial
growth factor A (VEGFA) (Wanleibio, WL00009b) primary antibody at
4 °C overnight, and then incubated with a secondary antibody
(ZSGBBI O, ZB2301) after washing. The signal of the immunoreactive
protein was visualized using a hypersensitive ECL chemiluminescence
reagent (Boster, AR1171) and then analyzed by using Image J software.

Mu X., Shi L., Pan S., He L., Niu Y, & Wang X. (2020). A Customized Self-Assembling Peptide Hydrogel-Wrapped Stem Cell Factor Targeting Pulp Regeneration Rich in Vascular-Like Structures. ACS Omega, 5(27), 16568-16574.

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Pathway analysis of fibroblast activation

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Cell lysates were separated by 10% SDS-PAGE. The specific primary antibodies used in Western blotting analysis are as follows: FAP (1:1000, ab53066, Abcam, UK), α-SMA (1:1000, ab5694, Abcam), IL11 (1:1000, ab89887, Abcam), IL15 (1:800, ab7213, Abcam); VEGFA (1:1000, wl00009b, Wanleibio, China); p-VEGFR2 (1:800, D155165, Sangon, China), T-VEGFR2 (1:1000, D151118, Sangon), p-KIT (1:800, D151515, Sangon), p-PDGFRβ (1:800, D151409, Sangon); FXR1 (1:1000, BS70701, bioworld, China); YAP1 (1:1000, sc-398182, Santa Cruz, USA); T-STAT3 (1:1000, #4904, CST, USA), p-STAT3 (1:1000, #9131, CST), T-AKT (1:1000, #9272, CST), p-AKT (1:1000, #2965, CST), T-ERK (1:1000, #9102, CST), p-ERK (1:1000, #4348, CST), T-P38 (1:1000, #9212, CST), p-P38 (1:1000, #4631, CST) and β-Actin (1:1500, ZSBIO, China). For ELISA assay, conditioned medium was collected from 1 × 10⁶ NFs or CAFs in a 6-well plate, and the concentrations of IL11 and IL15 were measured using a standard ELISA Kit (Raybiotech, USA) according to the manufacturer's instructions.

Du Y.E., Tu G., Yang G., Li G., Yang D., Lang L., Xi L., Sun K., Chen Y., Shu K., Liao H., Liu M, & Hou Y. (2017). MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling. Theranostics, 7(16), 3972-3988.

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Western Blot Analysis of Lung Tissue

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Lung tissue homogenates were lysed to extract total protein, quantified by the bicinchoninic acid assay (BCA) method. Fifty micrograms of total protein per sample were separated by SDS-PAGE and electro-transferred onto a nitrocellulose membrane. After blocking, primary antibodies raised against VEGFA (1:800, WL00009b, Wanleibio), VEGFR2 (1:300, sc-504; Santa Cruz Biotechnology Co., Ltd.), p-VEGFR2 (1:200 sc-16629-R Santa Cruz), Akt (1:500, 4691s; Santa Cruz), P-Akt (Ser473, 1:500, 4060 s; Cell Signaling Technology, USA), HIF-1α (1:800, 20960-1-AP; ProteinTech Group), β-actin (1:5000, BM0005; Boster, China), p-IκBα (1:1000; 2859, Cell Signaling Technology), and IκBα (1:1000, 9242; Cell Signaling Technology) were added, respectively, for overnight incubation at 4°C. After washing, the corresponding horseradish peroxidase-labeled secondary antibodies were added for 1.5 h at room temperature. Enhanced chemiluminescent detection was performed, followed by exposure to X-ray film and development. β-actin was used as an internal reference. Protein bands were quantitated with the Image-Pro Plus 7.0 software.

Li X., Chen Y., Wang L., Shang G., Zhang C., Zhao Z., Zhang H, & Liu A. (2016). Quercetin alleviates pulmonary angiogenesis in a rat model of hepatopulmonary syndrome. Brazilian Journal of Medical and Biological Research, 49(7), e5326.

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Protein Expression Analysis of VEGF and VEGFR1

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Ovary and kidney samples were homogenized and lysed in lysis buffer (KGP2100, KeyGEN BioTECH, China). Protein extract was collected and subjected to BCA protein assays (Thermo, USA). Thirty microgram of protein was separated by SDS-PAGE and subsequently transferred to PVDF membrane. Afterwards, the membranes were blotted with 10% skim milk for 1h at 37℃ and incubated with primary antibodies against VEGF (WL00009b, Wanleibio, China) and VEGFR1 (AF6204, A nity Biosciences, USA) overnight at 4°C. After incubation with the horseradish peroxidase-conjugated goat anti-rabbit IgG (ZB-2301, ZSGB-BIO, China) for 2h at room temperature, the membranes were visualized with an enhanced chemiluminescence plus kit (P10100, NCM Biotech, China) and Bio-Rad ChemiDoc MP imaging system. Band density was quanti ed using ImageJ software.

Zhang J., Huang J., He X., Li N., Miao Y., Li B., Shao X, & Wang N. (2022). Ginkgo biloba extract 761 reduces vascular permeability of the ovary and improves the symptom of ovarian hyperstimulation syndrome in a rat model. Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 38(2).

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Protein Expression Analysis of VEGF and VEGFR1

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