The cDNA coding for isoform 1 (transcript variant 1) of Slc8a3 was obtained from GenBank
(NM_001167920.1). The coding region of Slc8a3 was amplified by PCR using primers (TableS2 ) and subcloned to the pmEGFP-C1 vector using restriction enzymes NheI and BamHI (Addgene plasmid #36412). The modified vector plasmid was confirmed by DNA sequencing and plasmid DNA was extracted by endotoxin free plasmid extraction kit (TIANGEN, China). 2 µg plasmid DNA of modified vector was diluted into 200 µL jetPRIME® buffer (Polyplus-transfection, Illkirch, France) and mixed. 4 µL jetPRIME® was added into mixed solution and incubated for 10 min at room temperature. 200 µL of transfection mix was added per well dropwise onto the cells in serum containing medium. The plates were incubated at 37 °C for 6 h and then the medium was changed.
(NM_001167920.1). The coding region of Slc8a3 was amplified by PCR using primers (Table