Sodium periodate (NaIO4, > 99.8%) was obtained from Acros Organics (Fair Lawn, NJ). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA). Phosphate buffer saline (PBS), tetraethyl orthosilicate (TEOS, 99.8%), ethanol (200 proof), sodium hydroxide, and acetic acid (Glacier) were purchased from Fisher Scientific Co. (Pittsburgh, PA). Histology mounting medium Polyfreeze, Trichrome Stain (Masson) Kit, Bouin’s solution, and Weigert’s iron hematoxylin solution were purchased from Sigma-Aldrich (St. Louis, MO). Anti-CD163 antibody (ab87099), goat anti-rabbit IgG H&L (Alexa Fluor 488) (ab150077), anti-CD68 antibody (ab125212), and goat anti-rabbit IgG H&L (Alexa Fluor 647) were purchased from Abcam (Cambridge, MA). 4,6-Diamidino-2-phenylindole (DAPI) was obtained from Invitrogen (Grand Island, NY). PEG-DA (Figure S1 ) was prepared using an 8-armed PEG (MW = 20 kDa, JenKem USA, TX) while following previously published protocols (22 (link), 30 (link)).
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide 98 mtt
Manufactured by Thermo Fisher Scientific
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 98% (MTT) is a chemical compound used as a colorimetric assay for assessing cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to a purple formazan product by the action of dehydrogenase enzymes in metabolically active cells.
Automatically generated - may contain errors
Lab products found in correlation
Sodium periodate naio4, by Thermo Fisher Scientific (2 mentions)
Trichrome stain masson kit, by Merck Group (1 mentions)
Goat anti rabbit igg h l alexa fluor 488 ab150077, by Abcam (1 mentions)
Goat anti rabbit igg h l alexa fluor 647, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Bouin s solution, by Merck Group (1 mentions)
Polyfreeze, by Merck Group (1 mentions)
Weigert s iron hematoxylin solution, by Merck Group (1 mentions)
Cd68 antibody ab125212, by Abcam (1 mentions)
Sodium phosphate monobasic monohydrate, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Corning (1 mentions)
Dulbecco s modified eagle s medium dmem, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Concentrated hydrochloric acid, by Thermo Fisher Scientific (1 mentions)
Anhydrous dimethylformamide, by Thermo Fisher Scientific (1 mentions)
Dopamine hcl, by Thermo Fisher Scientific (1 mentions)
Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (1 mentions)
2 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide 98 mtt
1
Synthesis and Characterization of PEG-DA Hydrogel
2
Dopamine-PEG Conjugation for Tissue Engineering
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dopamine
HCl and N-methylmorpholine
were purchased from Acros Organics (Fair Lawn, New Jersey). The 4-arm
10k Da N-hydroxysuccinimide ester activated poly(ethylene
glycol) (PEG-NHS) was purchased from JenKem U.S.A., Inc. (Allen, TX).
Anhydrous dimethylformamide, sodium phosphate monobasic monohydrate,
sodium phosphate dibasic anhydrous, concentrated hydrochloric acid
(36.5–38%), and sodium periodate (NaIO4) were purchased
from Acros Organics (Fair Lawn, New Jersey). Fresh bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
Spectra/Por dialysis membrane (molecular weight cut off (MWCO): 3500
Da) was purchased from Spectrum Laboratories (Rancho Dominguez, California).
Dulbecco’s modified Eagle’s medium (DMEM; with 4.5 g/L
glucose and glutamine, without sodium pyruvate) and trypsin EDTA (0.05%
trypsin/0.53 mM EDTA in HBSS) were obtained from Corning Cellgro (Manassas,
VA). Fetal bovine serum and penicillin-streptomycin (10 units/mL)
were purchase from Thermo Scientific (Rockford, IL). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA).
HCl and N-methylmorpholine
were purchased from Acros Organics (Fair Lawn, New Jersey). The 4-arm
10k Da N-hydroxysuccinimide ester activated poly(ethylene
glycol) (PEG-NHS) was purchased from JenKem U.S.A., Inc. (Allen, TX).
Anhydrous dimethylformamide, sodium phosphate monobasic monohydrate,
sodium phosphate dibasic anhydrous, concentrated hydrochloric acid
(36.5–38%), and sodium periodate (NaIO4) were purchased
from Acros Organics (Fair Lawn, New Jersey). Fresh bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
Spectra/Por dialysis membrane (molecular weight cut off (MWCO): 3500
Da) was purchased from Spectrum Laboratories (Rancho Dominguez, California).
Dulbecco’s modified Eagle’s medium (DMEM; with 4.5 g/L
glucose and glutamine, without sodium pyruvate) and trypsin EDTA (0.05%
trypsin/0.53 mM EDTA in HBSS) were obtained from Corning Cellgro (Manassas,
VA). Fetal bovine serum and penicillin-streptomycin (10 units/mL)
were purchase from Thermo Scientific (Rockford, IL). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA).
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