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9609bnwp

Manufactured by Thermo Fisher Scientific
Sourced in United States

The 9609BNWP is a laboratory equipment product from Thermo Fisher Scientific. It is designed to perform a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Additional information is not available.

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5 protocols using 9609bnwp

1

Potentiometric Fluoride Quantification

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The fluoride levels in the plasma and kidney were estimated measured according to a potentiometric method1 (link) using Thermo Orion 720A+ with fluoride ion sensitive electrode 9609BNWP (Thermo, USA).
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2

Fluoride Analysis in Milk Samples

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For fluoride analysis, the milk containers were shaken briefly and gently, and a 3.0 ml aliquot was taken from each container. The fluoride concentration of the aliquot was then determined using a fluoride ion-selective electrode (ISE, 9609 BNWP, ThermoFisher Scientific, Fort Collins, CO) in conjunction with a pH/ISE meter (model 710, Thermo Orion, Beverly, MA). Five fluoride standards ranging from 0.1 ppm, 1 ppm, 5, ppm, 10 ppm, and 25 ppm were used to produce the calibration line18 (link),19 (link). To test effects of adjusting and stabilizing the total ion strength on the results, a set of experiments were also carried out by mixing 2.7 ml of milk samples with 0.3 ml of total ionic strength adjusting buffer (TISAB) III (Orion Ionplus, Chemlsford, MA) or TISAB I as recommended by the manufacturer to adjust the total ion strength and pH of the alternative milk beverages. All samples were analyzed three separate times to ensure the reliability and consistency.
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3

Fluoride Concentration Analysis Protocol

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All samples were analyzed for F concentration by a fluoride combination ion selective electrode (9609BNWP, Thermo Science, Waltham, MA) and an ion meter (930A, Thermo Science). The standard fluoride concentrations were 10, 1.0, and 0.1 ppm, and the solutions were prepared by dissolving NaF in the mixture of TISAB III at the ratio of 1–10. Fluoride concentrations were calculated by comparison with a standard curve using three standards ranging from 0.1 to 10 ppm standard.
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4

Fluoride Concentration Analysis by Ion Selective Electrode

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Liquid samples were analyzed for F concentration by a fluoride combination ion selective electrode (9609BNWP, Thermo Science, USA) and an ion meter (930A, Thermo Science, USA). The standard fluoride concentrations were 10, 1.0, and 0.1 ppm were prepared. TISAB III (Total ionic strength adjustment buffer III solution, Sigma–Aldrich Co. LLC., Tokyo, Japan) was added to each sample at the ratio of 10 to 1. Fluoride concentrations of samples were calculated by comparison with a standard curve using 3 standards ranging.
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5

Fluoride Concentration Measurement in Artificial Saliva

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Fluoride concentration in the artificial saliva was measured using an ion-selective electrode (9609BNWP, Thermo Fisher Scientific, Waltham, MA, USA) by mixing 0.5 mL artificial saliva with 0.5 mL TISAB solution (Thermo Fisher Scientific, Waltham, MA, USA). The data output was converted to mg/L fluoride ion with reference to calibration standards of 0.1, 0.5, 1, 2 and 5 mg/L F-, also diluted with equal quantities of TISAB.
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