Treated cells were collected by centrifugation, and the supernatant was discarded. The cells were washed twice with PBS and resuspended in 500 μl Binding Buffer (Wanleibio Co., Ltd., Shenyang, China). Next, 5 μL Annexin V-FITC (Wanleibio Co., Ltd.) and 10 μL propidium iodide (Wanleibio Co., Ltd.) were added and samples were mixed. Samples were incubated at room temperature away from light for 5-15 min. Analysis by flow cytometry was then performed.
Propidium iodide
Manufactured by Wanlei
Sourced in China
Propidium iodide is a fluorescent dye used in molecular biology and cell biology. It is commonly used to stain and identify dead cells in a population by binding to the DNA of cells with compromised cell membranes.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using propidium iodide
1
Annexin V-FITC and Propidium Iodide Apoptosis Assay
2
Cell Cycle Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each group of cells was washed twice with PBS; cells were harvested by centrifugation and resuspended in PBS, then fixed with 70% ethanol at 4°C for 2 hours. To remove ethanol, cells were washed twice in ice‐cold PBS, then resuspended in a solution containing 100‐μL RNase A and 500‐μL propidium iodide (Wanleibio) at 37°C for 30 minutes in the dark. Cell cycle was detected by flow cytometry (NovoCyte; Acea Biosciences, San Diego, CA).
3
Apoptosis Detection by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were collected and ltered through a 40-μm nylon cell strainer. The cells were washed with PBS and resuspended in binding buffer with Annexin V-FITC (Wanleibio, China) and Propidium Iodide (Wanleibio) at 4℃ for 15min and acquired on a FACS on CytoFLEX S (BECKMAN COULTER, USA). Data were analyzed using software (CytExpert 2.3).
4
Annexin V-Light 650 and PI Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were extracted and washed twice with phosphate-buffered saline (PBS). Then those cells were coated in 500 µl of binding buffer containing 5 µl of Annexin V-Light 650 and 5 µl of propidium iodide (PI; Wanleibio) at room temperature in the dark for 15 min. Flow cytometry with a NovoCyte cytometer (ACEA Biosciences, San Diego, CA, USA) was performed to detect the stained cells.
5
Cell Cycle Analysis of VSMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each group of VSMCs was washed twice with ice-cold phosphate buffered saline (PBS); cells were harvested by centrifugation and re-suspended in PBS, then fixed and permeabilized with 70% ethanol. Cells were washed twice in ice-cold PBS to remove ethanol, then treated with 500 μl propidium iodide (PI; Wanleibio, China) in the dark at 4°C for 30 min. Flow cytometry (ACEABio, NovoCyte, USA) was used to detect cell cycle.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!