Cell proliferation was determined using the CellTiter 96TM Aqueous non-radioactive cell proliferation kit (Promega) according to manufacturer instructions. Approximately 5000 cells were plated into 96-well plates in quintuplicate. Cells were treated with control (vehicle), Vorinostat (0.1, 0.25, 0.5, 1.0, 2.5, 5.0 and 10.0 μM), or cisplatin (2.5,5.0, 7.5, 10.0, 12.5, 15.0, 17.5 and 20.0 μg/ml) for 24 h. Cells were incubated with MTS at 37 °C for 4 h, and the results were assessed by absorbance (Bio-Tek EL-311, Bio-Tek Instruments) at 490 nm.
Celltiter 96tm aqueous non radioactive cell proliferation kit
Manufactured by Promega
The CellTiter 96TM Aqueous non-radioactive cell proliferation kit is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The kit utilizes a novel tetrazolium compound and an electron coupling reagent to produce a colored formazan product that is soluble in tissue culture medium.
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3 protocols using celltiter 96tm aqueous non radioactive cell proliferation kit
1
Cell Proliferation Assay with Vorinostat and Cisplatin
2
Determination of Cytotoxicity IC50 Values
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We used the CellTiter 96TM AQueous non-radioactive cell proliferation kit (Promega) to determine concentrations of Cisplatin and SAHA that inhibited cell proliferation by 50% (IC50). Cell proliferation was determined by reduction of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) following the manufacturer's protocol. In brief, 2500 cells were plated into 96-well plates and treated in triplicate with control (vehicle), SAHA (0.1, 0.25, 0.5, 1.0, 2.5, 5.0 and 10.0 μM), or Cisplatin (2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 17.5 and 20.0 μg/ml) for 24 h. Cells were incubated with MTS at 37°C for 4h, and the results were assessed by absorbance (Bio-Tek EL-311, Bio-Tek Instruments) at 490 nm.
3
Cell Proliferation Assay with Vorinostat and Cisplatin
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