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β actin antibody

Manufactured by GenScript
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The β-Actin antibody is a protein-specific antibody designed to detect the presence and quantity of the β-actin protein. β-Actin is a widely expressed cytoskeletal protein that plays a fundamental role in various cellular processes. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the β-actin protein.

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Lab products found in correlation

Ecl prime, by GE Healthcare (1 mentions) β tubulin ab6046, by Abcam (1 mentions) Cleaved caspase 3 5a1e, by Cell Signaling Technology (1 mentions) Triton x 100, by Merck Group (1 mentions) Gsk 3β, by Thermo Fisher Scientific (1 mentions) Luteolin, by Merck Group (1 mentions) Staurosporine, by Merck Group (1 mentions) Gsk3β ps9, by Thermo Fisher Scientific (1 mentions) Tau 5, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) 5 bromo 4 chloro 3 indolyl phosphate nitro blue tetrazolium bcip nbt, by Merck Group (1 mentions) Tdzd 8, by Merck Group (1 mentions) Alkaline phosphatase conjugated secondary antibodies, by Southern Biotech (1 mentions) β amyloid fragment peptide 1 42 aβ42, by AnaSpec (1 mentions) Kinase selectivity profiling assay kit, by Promega (1 mentions) Celltiter 96 aqueous one solution cell proliferation mtx assay kit, by Promega (1 mentions) Tau ps199 ps202, by Thermo Fisher Scientific (1 mentions) Adp glo kinase assay kit, by Promega (1 mentions) Hrp conjugated goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

β-actin (33 citations) Anti-β-actin antibody (5 citations) Mouse anti-β-actin monoclonal antibody (2 citations)

5 protocols using β actin antibody

1

Western Blotting of C. elegans RACK-1

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Nematode lysates were prepared as previously described.90 (link) Samples were separated by 10% Bis-Tris polyacrylamide gels and transferred onto PVDF membranes for western blotting. The polyclonal antibody against C. elegans RACK-1 amino acids 264–282 (IWDLEDKKEIEELKPEIAS) was raised in rabbits by LTK BioLaboratories, and unprocessed immune serum was used at a 1:5000 dilution to detect RACK-1 as a single band. Actin was detected by monoclonal mouse β-Actin antibody (A00702, GenScript, 1:1000 dilution). Horseradish peroxidase-conjugated anti-rabbit (211-032-171, Jackson ImmunoResearch Labs, 1:10 000 dilution) or anti-mouse (115-035-174, Jackson ImmunoResearch Labs, 1:10 000 dilution) light-chain-specific secondary antibodies were used for signal detection by ECL Prime (GE Healthcare). Images were acquired by UVP BioSpectrum 500 imaging system.
Chu Y.D., Wang W.C., Chen S.A., Hsu Y.T., Yeh M.W., Slack F.J, & Chan S.P. (2014). RACK-1 regulates let-7 microRNA expression and terminal cell differentiation in Caenorhabditis elegans. Cell Cycle, 13(12), 1995-2009.
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2

Phosphorylation-specific MCM2 Antibody Protocol

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Anti-pSer40/41MCM2 antibody was raised by GenScript (GenScript USA Inc) using a CAPLT(p)S(p)SPGR peptide and further affinity purified. Cleaved PARP (19F4) and cleaved Caspase 3 (5A1E) antibodies were from Cell Signalling Technology; CDC7 (clone SMP171) and β-tubulin (ab6046) antibodies were from Abcam; β-Actin antibody (Cat No. A00702) was from GenScript; MCM2 antibody (Cat No. MCA1859) was from AbD Serotec; pSer41MCM2 antibody (Cat no. A300-117A-1) was from Bethyl Laboratories Inc; γ-H2AX antibody, recognizing pSer139-H2AX (Cat No. 05-636) was from Millipore; pSer108MCM2 antibody was previously described [9] (link). Secondary antibodies labeled with infrared fluorophores (800CW anti-rabbit Cat No. 926-32211, 800CW anti-mouse Cat no. 926-32210, 680LT anti-rabbit Cat No. 926-68021 and anti-mouse Cat no. 926-68020) were obtained from Li-COR Biosciences Ltd, secondary antibodies labeled with TRITC fluorophore were obtained from Jackson ImmunoResearch. Odyssey Infrared Imaging Systems were from Li-COR Biosciences Ltd.
FitzGerald J., Murillo L.S., O'Brien G., O'Connell E., O'Connor A., Wu K., Wang G.N., Rainey M.D., Natoni A., Healy S., O'Dwyer M, & Santocanale C. (2014). A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE, 9(6), e98891.
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3

APP, PS1, and β-Actin Detection in Detergent-Insoluble Lysates

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Each hemicortex was weighed and homogenized on ice in 8 volume of lysis buffer (50 mM Tris, pH7.4, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, and 0.5% sodium deoxycholate) using a Kontes homogenizer. The homogenates were centrifuged at 16,000 g for 20 min at 4 °C, and the supernatant fractions were saved as the detergent-soluble lysates. 100 μg of the detergent-insoluble lysates was resolved on a Tris-tricine gel or on a 10% SDS-PAGE to detect APP fragments, PS1 fragments and β-actin. 6E10 (Bio legend) was used to detect human specific full-length APP, soluble APPα, CTFβ, while Aβ. C1/6.1 (Biolegend) was used to detect both mouse and human full-length APP and CTFα and CTFβ. PS1 NT1 (Biolegend) was used to detect PS1, and β-actin antibody (Genscript) was used to detect β-actin.
Shaik S.M., Cao Y., Gogola J.V., Dodiya H.B., Zhang X., Boutej H., Han W., Kriz J, & Sisodia S.S. (2023). Translational profiling identifies sex-specific metabolic and epigenetic reprogramming of cortical microglia/macrophages in APPPS1-21 mice with an antibiotic-perturbed-microbiome. Molecular Neurodegeneration, 18, 95.
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4

Alzheimer's Disease In Vitro Assays

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Staurosporine, 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD-8), lithium chloride (LiCl), luteolin, Triton X-100, protease inhibitor cocktail, and 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT) reagents were from Sigma-Aldrich (Saint Louis, MO, USA). β-Amyloid fragment peptide 1–42 (Aβ42) was from AnaSpec (Fremont, CA, USA). Kinase Selectivity Profiling Assay Kit, ADP-Glo Kinase Assay Kit, and CellTiter 96 AQueous One Solution Cell Proliferation MTX Assay Kit were from Promega (Madison, WI, USA). Antibodies Tau-5, Tau pS199/pS202, Tau pS396, GSK3β, and GSK3β pS9 were from Invitrogen (Camarillo, CA, USA). β-Actin antibody was from GenScript (Piscataway, NJ, USA). Alkaline phosphatase-conjugated secondary antibodies were from Southern Biotech (Birmingham, AL, USA).
Liang Z., Zhang B., Su W.W., Williams P.G, & Li Q.X. (2016). C-Glycosylflavones Alleviate Tau Phosphorylation and Amyloid Neurotoxicity through GSK3β Inhibition. ACS chemical neuroscience, 7(7), 912-923.
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5

Immunoblotting of key muscle proteins

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Unless otherwise mentioned chemical reagents were purchased from Sigma-Aldrich (Saint Louis, MO). Primary DHPR α1 (Cav1.1), Mouse RyR1 (clone 34C) and G3PD (GAPDH) antibodies and secondary goat anti-mouse IgG HRP-conjugated antibody were bought from Thermo Scientific (Rockford, IL). anti-MHCIIa-FITC antibody was from Biorbyt (San Francisco, CA). β-actin antibody was bought from GenScript (Piscataway, NJ). Synaptobrevin (VAMP1) mouse antibody was bought from Invitrogen (Carlsbad, CA).
Ivannikov M.V, & Van Remmen H. (2015). Sod1 gene ablation in adult mice leads to physiological changes at the neuromuscular junction similar to changes that occur in old wild type mice. Free radical biology & medicine, 84, 254-262.
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