V450 mouse anti human cd44

For flow cytometry analyses and cell sorting tumor spheres were dissociated as single cells, washed and incubated with the appropriate dilution of control or specific antibody. Antibodies used were V450 Mouse Anti-Human CD44 (561292), PE-Cy7 Mouse Anti-Human CD45 (557748), PE Mouse Anti- Human CD146 (550315), PE-Mouse Anti-Human CD24 (555428), FITC Mouse Anti-Human CD90 (561969), FITC-Mouse Anti-Human EpCAM (347197), APC-Mouse Anti-Human CD10 (332777), (all from BD Bioscience Bedford, Franklin Lakes, NJ) and Mouse monoclonal antibody [5D3] to Cytokeratin 8 + 18 (Abcam Cambridge,UK, 17139). After 45 min incubation, cells were washed. Analysis was performed using a FACS Canto flow cytometer (BD Biosciences). Cell Sorting was performed by FACS ARIA cytometer equipped with three lasers (488, 633, 407 nm) (BD Biosciences). All the cytofluorimetric acquisitions were analyzed by BD FACSDiva Software version 6.1.3 (BD Biosciences). For the sorting, cells were incubated with antibodies for 1 h then washed in PBS. Antibodies were used following manufacturing protocol indication for sorting experiment. TO-PRO3 (Thermo Fisher) dye was used for viability evaluation and used following manufacturing protocol indication. All the cytofluorimetric acquisitions were analyzed by BD FACSDiva Software version 6.1.3 (BD Biosciences).