Sprague-Dawley (SD) rats were purchased from Nara biotech (Gangnam-gu, Seoul, Korea). All animal experiments were in accordance with the guidelines of the Kyung Hee University Institutional Animal Care and Use Committee (KHUASP (SE)-14-032). The rats were housed under controlled temperature (23 ± 3°C) with a relative humidity of 40-60% and 12-h light/dark cycles. Food and water were provided ad libitum.
Sprague dawley rats
Manufactured by Nara Biotech
Sourced in United States
Sprague Dawley rats are a commonly used rodent model in biomedical research. They are an outbred strain of albino rats that are known for their docile temperament and reliable breeding characteristics. Sprague Dawley rats are widely used in various research applications, including studies on metabolism, toxicology, and drug development.
Automatically generated - may contain errors
20 protocols using sprague dawley rats
1
Housing and Care of Sprague-Dawley Rats
2
Rat Biofluid Extraction for Physiological Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five-week-old female Sprague Dawley (SD) rats weighing around 150 g were obtained from Nara Biotech Co., Ltd. (Seoul, Korea) and acclimated to environments for seven days before experiment. Animals were housed in plastic animal cages in a ventilated room maintained at 20 ± 2 °C and 60 ± 10% relative humidity under a 12 h light/dark cycle. Water and commercial laboratory complete feed for rats were provided ad libitum. All animal experiments were performed in accordance with the approved animal protocol and guideline established by the Animal and Ethics Review Committee of Seoul Women’s University (IACUC-2016A-3).
Rat biofluids, such as GI fluids and plasma, were obtained as previously reported [50 ]. Briefly, stomachs and small intestines were collected and rinsed with saline, and then gastric and intestinal fluids were obtained by centrifugation at 16,000× g for 15 min at 4 °C. To obtain plasma, blood sample was collected via tail vein using a catheter, and centrifuged at 16,000× g for 3 min at 4 °C.
Rat biofluids, such as GI fluids and plasma, were obtained as previously reported [50 ]. Briefly, stomachs and small intestines were collected and rinsed with saline, and then gastric and intestinal fluids were obtained by centrifugation at 16,000× g for 15 min at 4 °C. To obtain plasma, blood sample was collected via tail vein using a catheter, and centrifuged at 16,000× g for 3 min at 4 °C.
3
Sabinene Administration and Gastrocnemius Muscle Analysis in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH publication No. 85-23, revised 1996). They were approved by the Animal Subjects Committee following institutional guidelines of Konkuk University, Korea. Sprague-Dawley (SD) rats (8-week-old, male, 250–300 g; Nara Biotech, Seoul, Korea) were divided into three groups (n = 10 per group). One group of rats were orally administered sabinene once daily for 2 days (6.4 mg/kg body weight) and Tween-80 (1%) in saline solution. The other two groups of rats were treated with saline solution containing Tween-80 (1%) with or without food pellets. In fasted groups, food pellets were removed from cages of rats to be fasted. Two days later, rats were anesthetized intraperitoneally with Zoletil® (40 mg/kg body weight; Virbac Laboratories, Carros, France) and Rompun® (10 mg/kg body weight; Bayer Korea, Seoul, Korea). The adequacy of anesthesia was determined by the lack of reflex response to foot pinching in anesthetized rats. Gastrocnemius muscles of rats were dissected and isolated for analysis.
4
Animal Protocol for Ophthalmic Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sprague Dawley (SD) rats with a mean weight of 250 g (5 weeks old; Nara Biotech, Seoul, Korea) were used in these studies. All animal studies were approved by the SKKU School of Pharmacy Institutional Animal Care and Use Committee in accordance with the ethical guidelines of the SKKU School of Pharmacy and the Association for Research in Vision and Ophthalmology statement regarding the use of animals in ophthalmic and vision research. The animals were maintained under a controlled 12-h light/dark cycle at an ambient temperature and had free access to water and standard rodent chow.
5
Osteoporosis Model in Ovariectomized Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vivo experiments were approved by the Institutional Animal Care and Use Committee at the Kyung Hee University Laboratory Animal Center (approval number: KHUASP (SE)-13–051). Forty 12-week-old, healthy, female Sprague-Dawley (SD) rats (Nara biotech, Seoul, Korea) were acclimatized for one week. After anesthesia with isoflurane, both ovaries were removed to complete a postmenopausal osteoporosis model. In addition, the sham-operated group was given the same surgical stress, but the ovaries were not ablated. The rats were randomly divided into 5 groups of 8 rats as follows:
(1) sham-operated + distilled water via oral administration;
(2) OVX + distilled water via oral administration;
(3) OVX + 17β-estradiol (E2, 100 μg/kg) via oral administration;
(4) OVX + low concentration of CF (CF-L, 35 mg/kg) via oral administration; and.
(5) OVX + high concentration of CF (CF-H, 350 mg/kg) via oral administration. Oral administration was carried out every morning for 8 weeks. At the end of the treatment, rats were anesthetized with pentobarbital sodium (80 mg/kg) and sacrificed by collecting blood close to the lethal dose with a cardiac puncture. The ovaries and femur were collected and weighed. Femurs were fixed with 10% neutral buffered formalin (NBF) overnight and were then stored at room temperature until used in the experiment.
(1) sham-operated + distilled water via oral administration;
(2) OVX + distilled water via oral administration;
(3) OVX + 17β-estradiol (E2, 100 μg/kg) via oral administration;
(4) OVX + low concentration of CF (CF-L, 35 mg/kg) via oral administration; and.
(5) OVX + high concentration of CF (CF-H, 350 mg/kg) via oral administration. Oral administration was carried out every morning for 8 weeks. At the end of the treatment, rats were anesthetized with pentobarbital sodium (80 mg/kg) and sacrificed by collecting blood close to the lethal dose with a cardiac puncture. The ovaries and femur were collected and weighed. Femurs were fixed with 10% neutral buffered formalin (NBF) overnight and were then stored at room temperature until used in the experiment.
6
Female Sprague Dawley Rat Housing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five-week-old female Sprague Dawley (SD) rats weighing 130–150 g were purchased from Nara Biotech, Co., Ltd (Seoul, South Korea). Animals were housed in plastic laboratory animal cages in a ventilated room maintained at 20 ± 2°C and 60 ± 10% relative humidity under a 12 h light/dark cycle. Water and commercial laboratory complete food were provided ad libitum. Animals were allowed to acclimate to the environment for 7 days before treatment. All animal experiments were performed in compliance with the guideline issued by the Animal and Ethics Review Committee of Seoul Women’s University and Ethics Review Committee of Seoul Women’s University approved the procedures used in this study (IACUC-2014A-3).
7
Sprague-Dawley Rat Care and Handling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thirty 8-week-old female Sprague-Dawley rats (weight, 250–300 g) were purchased (Nara Biotech, Pyeongtaek, Korea). All animal handling and surgical procedures were in compliance with guidelines for the care and use of laboratory animals published by the Institutional Animal Care and Use Committee of Bucheon St. Mary's Hospital (BSM 14-004) and the Animal Research: Reporting of In Vivo Experiments checklist. Animals were kept at a controlled temperature (25°C±1°C) with humidity of 55% and lighting conditions of a 12-/12-hour light/dark cycle, with unrestricted access to food and water.
8
Sprague Dawley Rat Housing and Acclimation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five-week-old male and female Sprague Dawley rats weighing 120–140 g were purchased from Nara Biotech Co., Ltd. (Seoul, South Korea). Animals were housed in plastic animal cages in a ventilated room maintained at 20°C±2°C and 60%±10% relative humidity, under a 12 hour light/dark cycle. Water and commercial laboratory complete food for rats were made available ad libitum. Animals were acclimated to this environment for 7 days before treatment. All animal experiments were performed after obtaining approval from the Animal and Ethics Review Committee of Seoul Women’s University.
9
Sprague-Dawley Rat Model for Ophthalmic Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The research conducted was approved by the Institutional Animal Care and Use Committee (IACUC) at Chungbuk National University (Approval No. CBNUA-2032-22-01), in accordance with the Association for Research in Vision and Ophthalmology statement for the Use of Animals in Ophthalmic and Vision Research.
Twenty-five male Sprague-Dawley rats, aged 8 weeks, were obtained from Nara Biotech (Pyeongtaek, Republic of Korea). They were housed in a conventional environment with a standard 12 h light/12 h dark cycle. The rats had free access to normal pellet chow (Experimental Rat & Mouse Diet, Purina, St. Louis, MO, USA) and water.
Twenty-five male Sprague-Dawley rats, aged 8 weeks, were obtained from Nara Biotech (Pyeongtaek, Republic of Korea). They were housed in a conventional environment with a standard 12 h light/12 h dark cycle. The rats had free access to normal pellet chow (Experimental Rat & Mouse Diet, Purina, St. Louis, MO, USA) and water.
10
Pharmacokinetic Study of Sprague-Dawley Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twelve male Sprague-Dawley rats (280±20 g, 7–9 weeks old) were purchased from the Nara Biotech (Seoul, South Korea). Prior to the experiments, the animals were freely provided with standard laboratory food and water. During all of the procedures, the animals were housed in cages under maintained temperature (23–26 °C) and relative humidity (50–55%) environmental conditions. Food was removed approximately 12 hr before the commencement of the pharmacokinetic procedures, but the animals were allowed free access to water. All animal care and procedures were conducted according to the Guiding Principles in the Use of Animals in Toxicology, as adopted in 1989, revised in 1999, and amended in 2008 by the Society of Toxicology (SOT). The protocols for the animal studies were also approved by the Institutional Animal Care and Use Committee (IACUC) at Hanyang University.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!