Zetasize nanozs

The structural integrity of aposomes in different storage conditions for extended periods was evaluated by size, polydispersity index, and zeta potential measurements every 24 h for 4 days. We analyzed aposomes made from FPLC fraction 12 and 13 and compared them to liposomes. For each sample, biological replicates were measured and for each replicates, half of the volume of particles was placed into room temperature storage and the other half into 4 °C storage. All samples were measured using Malvern Zetasize Nano ZS. Size and polydispersity index (P.D.I) were measured in a low volume disposable sizing cuvette (Malvern) with five ul of particles diluted in 495 ul of water 173 backscatter measurement angle. Measurement parameters were set to three measurements consisting of 10 runs of 10 s each with a general-purpose (standard resolution) analysis model. Zeta potential was measured using 10 ul of particles +900ul of deionized water 1 ml in a clear disposable zeta cell (Malvern) with measurement parameters set at three measurements, each with 20 runs using an “auto mode” analysis model.

The ability of the BBE and the BE to interact with the mucin was evaluated by means of the droplet size and the zeta potential, which were measured before and after contact with mucin solutions at different concentrations (200, 250, and 350 µg·mL⁻¹) [26 (link)].
Initially, the droplet size and the zeta potential were evaluated using a ZetaSize NanoZS (Malvern Instruments, Malvern, UK). Then, 50 µL of the emulsions were added in 10 mL of the mucin solutions and kept for 1 h at 37 °C under moderate magnetic stirring. Subsequently, the droplet size and the zeta potential were reevaluated under the same dilution condition as described in Section 2.2.6. All analyses were performed in triplicate.