Log In Sign up
The largest database of trusted experimental protocols

Iacs 010759

Manufactured by Chemietek
Visit Supplier

IACS-010759 is a high-precision laboratory instrument designed for scientific analysis and research applications. It features advanced technology and components to provide accurate and reliable measurements. The core function of this product is data collection and analysis, but a detailed description of its intended use is not available.

Automatically generated - may contain errors

Lab products found in correlation

Pbs protease inhibitor cocktail, by Roche (2 mentions) Type 2 collagen, by Chondrex (2 mentions) Qrt pcr, by Roche (2 mentions) Trametinib, by Selleck Chemicals (1 mentions) Pd0325901, by Selleck Chemicals (1 mentions) Palbociclib, by Pfizer (1 mentions) Etomoxir, by Merck Group (1 mentions) Wzb117, by Bio-Techne (1 mentions)

4 protocols using iacs 010759

1

Collagen-Induced Arthritis in Mice

Cited 5 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal studies were approved by the UIC Animal Care and Use Committee. Eight-week-old DBA1 mice were immunized with collagen type II (Chondrex, Redmond, WA) on days 0 and 21 [55 (link)–58 ]. CIA mice were treated i.p. 3 times weekly with 2-DG (7.5mg/kg, Sigma), IACS-010759 (10mg/kg; ChemieTek) [19 (link)] or control starting on day 25 until day 51 [55 (link),56 (link),59 (link),60 (link),57 (link),58 ]. Mice were sacrificed on day 52 post-onset and ankle circumference was measured with Caliper [59 (link)]. At the end of the experiment, ankles were dissected and homogenized for qRT-PCR (Trizol), ELISA (PBS+ protease inhibitor cocktail; Roche Diagnostics), or processed for immunohistochemistry (10% formalin).
Umar S., Palasiewicz K., Volin M.V., Romay B., Rahat R., Tetali C., Arami S., Guma M., Ascoli C., Sweiss N., Zomorrodi R.K., O’Neill L.A, & Shahrara S. (2021). Metabolic regulation of RA macrophages is distinct from RA fibroblasts and blockade of glycolysis alleviates inflammatory phenotype in both cell types. Cellular and molecular life sciences : CMLS, 78(23), 7693-7707.
+ Open protocol
+ Expand
2

Evaluating Novel Therapeutic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Trametinib and PD0325901 (22 (link)) were purchased from SelleckChem (Houston, TX). Palbociclib was from Pfizer (New York, NY). IACS-010759 was purchased from ChemieTek (Indianapolis, IN). All drugs were dissolved according to manufacturers’ instructions.
Teh J.L., Purwin T.J., Han A., Chua V., Patel P., Baqai U., Liao C., Bechtel N., Sato T., Davies M.A., Aguirre-Ghiso J, & Aplin A.E. (2020). Metabolic adaptations to MEK and CDK4/6 co-targeting in uveal melanoma. Molecular cancer therapeutics, 19(8), 1719-1726.
+ Open protocol
+ Expand
3

Dissolution of Chemical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
WZB117 (Tocris, Minneapolis, MN), 6-aminonicotinamide (Cayman Chemical Company, Ann Arbor, MI), Etomoxir (Sigma-Aldrich, St. Louis, MO), IACS-010759 (ChemieTek, Indianapolis, IN) were dissolved in DMSO.
Han A., Purwin T.J., Bechtel N., Liao C., Chua V., Seifert E., Sato T., Schug Z.T., Speicher D.W., Harbour J.W, & Aplin A.E. (2020). BAP1 mutant uveal melanoma is stratified by metabolic phenotypes with distinct vulnerability to metabolic inhibitors. Oncogene, 40(3), 618-632.
+ Open protocol
+ Expand
4

Collagen-Induced Arthritis in Mice

Cited 5 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal studies were approved by the UIC Animal Care and Use Committee. Eight-week-old DBA1 mice were immunized with collagen type II (Chondrex, Redmond, WA) on days 0 and 21 [55 (link)–58 ]. CIA mice were treated i.p. 3 times weekly with 2-DG (7.5mg/kg, Sigma), IACS-010759 (10mg/kg; ChemieTek) [19 (link)] or control starting on day 25 until day 51 [55 (link),56 (link),59 (link),60 (link),57 (link),58 ]. Mice were sacrificed on day 52 post-onset and ankle circumference was measured with Caliper [59 (link)]. At the end of the experiment, ankles were dissected and homogenized for qRT-PCR (Trizol), ELISA (PBS+ protease inhibitor cocktail; Roche Diagnostics), or processed for immunohistochemistry (10% formalin).
Umar S., Palasiewicz K., Volin M.V., Romay B., Rahat R., Tetali C., Arami S., Guma M., Ascoli C., Sweiss N., Zomorrodi R.K., O’Neill L.A, & Shahrara S. (2021). Metabolic regulation of RA macrophages is distinct from RA fibroblasts and blockade of glycolysis alleviates inflammatory phenotype in both cell types. Cellular and molecular life sciences : CMLS, 78(23), 7693-7707.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!