Anti cd27 percp cy5

Manufactured by BioLegend

Sourced in United Kingdom

The Anti-CD27-PerCP-Cy5.5 is a fluorescently-labeled antibody that binds to the CD27 protein, which is expressed on the surface of various immune cells. This product is intended for use in flow cytometry applications to identify and analyze CD27-positive cell populations.

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4 protocols using anti cd27 percp cy5

Profiling mRNA Translation in Leukemia

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mRNA translation was analyzed using O-propargyl-puromycin (OPP)-labeling and polysome profiling, as described [22 (link)]. For analysis of CLL samples, cells were stained with anti-CD5-PerCyP5.5 and anti-CD19-pacific blue antibodies (BD Biosciences) for 15 min on ice and OPP-labeling was quantified in CD5⁺CD19⁺ cells. For PBMCs from healthy donors, cells were stained with anti-CD5-APC-Cy7, anti-CD19-pacific blue, anti-CD27-PerCP-Cy5.5 and anti-IgG-FITC antibodies (all Biolegend) for 15 min on ice, and OPP-labeling was quantified separately on CD5⁻CD19⁺IgG⁻CD27⁻ and CD5⁻CD19⁺IgG⁻CD27⁺ cells. Polysome profiling was performed as described [22 (link)].

Wilmore S., Rogers-Broadway K.R., Taylor J., Lemm E., Fell R., Stevenson F.K., Forconi F., Steele A.J., Coldwell M., Packham G, & Yeomans A. (2021). Targeted inhibition of eIF4A suppresses B-cell receptor-induced translation and expression of MYC and MCL1 in chronic lymphocytic leukemia cells. Cellular and Molecular Life Sciences, 78(17-18), 6337-6349.

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Comprehensive Immunophenotyping of Immune Cells

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Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).

Reincke M.E., Payne K.J., Harder I., Strohmeier V., Voll R.E., Warnatz K, & Keller B. (2020). The Antigen Presenting Potential of CD21low B Cells. Frontiers in Immunology, 11, 535784.

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Flow Cytometric Immunophenotyping of Lymphocytes

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The following antibodies were used for flow cytometric stainings: anti-CD19 APC-Cy7, anti-CD27 PerCP-Cy5.5, anti-CD21 Pacific Blue, anti-CD4 APC-Cy7, anti-CD25 PE- Cy7, PE or PE Dazzle, anti-CD127 PerCP-Cy5.5 or APC, anti-CD45RO AF-700, anti-CXCR5 Pacific Blue, anti-PD-1 PE-Cy7 (all from BioLegend), anti-CD3 eFluor 605NC (eBioscience) and anti-IgG APC (Becton Dickinson). Intracellular staining with anti-Foxp3 Alexa Fluor 488 (eBioscience) or APC (Biolegend) was performed using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) in accordance with manufacturer instructions.

Romberg N., Le Coz C., Glauzy S., Schickel J.N., Trofa M., Nolan B.E., Paessler M., Xu M.L., Lambert M., Lakhani S.A., Khokha M.K., Jyonouchi S., Heimall J., Takach P., Maglione P.J., Catanzaro J., Hsu F.I., Sullivan K.E., Cunningham-Rundles C, & Meffre E. (2018). CVID patients with autoimmune cytopenias exhibit hyperplastic yet inefficient germinal center responses. The Journal of allergy and clinical immunology, 143(1), 258-265.

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Multiparameter Immune Cell Phenotyping

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Anti-CD8a-eFluoro® 450, anti-CD127-PE-Cy7, anti-IFNγ-eFluoro® 450, anti-TNFα-FITC were obtained from eBioscience (San Diego, USA), anti-CD44-FITC, anti-CD62L-alexa700 were obtained from BD Biosciences (Oxford, UK), and anti-CD27-PerCP-Cy5.5 was obtained from Biolegend (San Diego, USA). Cells were incubated with the indicated antibodies at 4°C for 20 minutes.

Colston J.M., Bolinger B., Cottingham M.G., Gilbert S, & Klenerman P. (2016). Modification of antigen impacts on memory quality after Adenovirus vaccination. Journal of immunology (Baltimore, Md. : 1950), 196(8), 3354-3363.

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