Hnrnpd

The following antibodies were used: HNRNPD (1:1000, 07-260, Millipore, RRID:AB_2117338), HNRNPD (1:1000, D6O4F, Cell Signalling, Danvers, MA, RRID:AB_2616009), RPA32 (1:5000, A300–244A, Bethyl Laboratories, RRID:AB_185548), RPA32 S4/S8 (1:2000, A300–245A, Bethyl Laboratories), H3 (1:1000, #9715, Cell Signalling, RRID:AB_331563), CHK1 S345 (1:1000, #2348, Cell Signalling), CHK1 (1:1000, #2360, Cell Signalling), GAPDH (1:1000, sc-25778, Santa Cruz, Dallas), MRE11 (1:1000, NB100–142, Novus Biological), EXOI (1:1000, A302–640A, Bethyl Laboratories), CtIP (1:1000, #61142, Active Motif) SAF-A (1:1000, ab10297, Abcam), RAD17 S645 (1:2000, ab3620, Abcam), FLAG-M2 (1:1000, F1804, SIGMA Aldrich), HA-tag (1:500, sc-805, Santa Cruz Biotechnology), Lamin A/C (1:1000, #4777, Cell Signalling), GFP (1:5000, ab6556, Abcam), His-tag (1:1000, 05-531, Millipore). For total protein extraction, cells were lysed at 4°C in 50 mM HEPES pH7.5, 1% Triton X-100, 150 mM NaCl, 5 mM EGTA, supplemented with protease and phosphatase inhibitor cocktail (Roche Applied Science). Lysates were clarified by centrifugation at 10 000 × g for 20 min. Lysates containing equal amounts of proteins, estimated through the Bradford assay (Bio-Rad), were subjected to SDS-page. The chemiluminescent images were obtained using the ImageQuant LAS 500 (GE Healthcare).

Western blot analysis was performed as previously described^{32 (link)}. Rabbit anti‐heterogeneous nuclear ribonucleoprotein D (HNRNPD) antibody (12382S, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti‐Y-box binding protein-1 (YBX1) (9744S, Cell Signaling Technology) antibody were used for western blot. The dilution of primary antibodies was 1:1000. An anti-GAPDH antibody was used as the endogenous loading control.