Rabbit anti caspase 3 antibody

Manufactured by Merck Group

Sourced in United States, Germany

Rabbit anti-caspase-3 antibody is a laboratory reagent used to detect and study the expression and activity of caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody is produced in rabbits and can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to facilitate research on cellular processes related to apoptosis.

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Caspase-3 (76 citations) Anti-caspase-3 (33 citations) Rabbit anti-caspase 3 (11 citations) Anti-caspase-3 antibody (6 citations) Rabbit anti- (2 citations)

2 protocols using rabbit anti caspase 3 antibody

Investigating Anti-Cancer Mechanisms

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All cell culture media, antibiotics, and trypsin were purchased from Gibco (Grand Island, NY, USA), and foetal bovine serum (FBS) was purchased from HyClone (Logan, UT, USA). Rabbit anti-NF-κΒ p65 antibody, rabbit anti-IKKα antibody, rabbit anti-IκΒα antibody, rabbit anti-caspase-8 antibody, rabbit anti-GAPDH antibody, rabbit anti-Poly[ADP-ribose] Polymerase (PARP) antibody, rabbit anti-cyclin B1 antibody, rabbit anti-survivin antibody, rabbit anti-caspase-3 antibody, mouse anti-α-tubulin antibody, rabbit anti-β-actin antibody, goat anti-rabbit IgG-peroxidase, goat anti-mouse IgG-peroxidase, goat anti-rabbit IgG-FITC, goat anti-mouse IgG-FITC, DAPI, methyl thiazolyl tetrazolium (MTT), and Taxol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Immobilon membranes were purchased from Merck Millipore (Bedford, MA, USA). Cell Tracker CM-Dil and Calcein-AM were purchased from Invitrogen (Carlsbad, CA, USA). ECL Plus substrate, bicinchoninic acid (BCA) reagents, and RIPA lysis buffer were purchased from CWBio (Beijing, China). Kanglaite injection was purchased from Zhejiang Kanglaite Pharmaceutical Co., Ltd, (Zhejiang, China).

Wang Y., Zhang C., Zhang S., Zhao Z., Wang J., Song J., Wang Y., Liu J, & Hou S. (2017). Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation. Scientific Reports, 7, 1280.

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Collagenase-Based Protein Extraction Assay

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Collagenase was obtained from Serva Feinbiochemica (Heidelberg, Germany); BSA (bovine serum albumin) fraction V, rabbit anti-caspase-3 antibody, mouse monoclonal anti β-actin antibody and other reagents were from Sigma-Aldrich. Anti-Bcl-2 (rabbit polyclonal, sc-492), and anti-microtubule-associated protein light chain 3 (LC3; goat polyclonal, sc 16755) antibodies were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Polyclonal rabbit antiserum to VMP1 was developed by Vaccaro's laboratory [9] . Alexa Fluor 488 and 594 antibodies (Molecular Probes) were used for immunofluorescence assays. Peroxidase-labeled anti-rabbit and anti-goat IgG antibodies were used for Western blot according to GE Healthcare.
Exendin-4 was obtained from Bachem California Inc. (Torrance, CA, USA) while sitagliptin was kindly provided by Process Research, Merck Research Laboratories.

Maiztegui B., Boggio V., Román C.L., Flores L.E., Zotto H.D., Ropolo A., Grasso D., Vaccaro M.I, & Gagliardino J.J. (2017). VMP1-related autophagy induced by a fructose-rich diet in β-cells: its prevention by incretins. Clinical science (London, England : 1979), 131(8).

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