P ezrin

Antibodies against Ezrin, p-Ezrin, E-cadherin, Vimentin, Snail, p-S6, p-Akt, p-4EBP-1, S6, Akt, 4EBP-1 and β-Actin were purchased from Cell Signaling Technology (Boston, USA). Cytokeratin 18, HPV18 E6 and E7 antibody was purchased from Santa Cruz (Dallas, USA). Antibody against MMP-2 was purchased from BOSTER (Wuhan, China). MMP-9 and β-cantenin were purchased from Affinity (Cincinnati, USA). Antibody against Twist was purchased from Abcam (Cambridge, UK).

Purified splenic B cells (2 × 10⁶) were activated at 37°C with sAg for 5, 10 and 30 min. Cell lysates were obtained using a mixed RIPA buffer, as previously described.²⁴ Lysates were run through SDS‐PAGE and western blotting. Abs from Cell Signaling Technology: BTK (8547S), pAKT (4060L), AKT (9272S), SHIP‐1 (2728S), pFOXO1 (9461S), FOXO1 (2880S), pS6 (4856S), S6 (2217S), pPI3K (4228S), PI3K (4292S), pMST1 (3681S), MST1 (PA5‐22015), pmTOR (5536S), mTOR (2983S), pEZRIN (3726S), STAT1 (14994S), P65 (4764S), pIKKB (2697S), IKKB (8943S), and anti‐human‐CCR2 (12199S). From Abcam: STAT5 (ab194898). From Santa Cruz Biotechnology: WASP (sc‐13139). Loading controls: anti‐mouse β‐actin (60008‐1‐IG‐10, Proteintech), anti‐human GAPDH (5174S, Cell Signaling Technology). Western blotting imaging was performed using the ChemiDoc™XRS + imaging systems (Bio‐Rad).