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Mouse hemoglobin a1c kit

Manufactured by Crystal Chem
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The Mouse Hemoglobin A1c Kit is a laboratory instrument designed to measure the glycated hemoglobin (HbA1c) levels in mouse samples. HbA1c is a widely used biomarker for assessing long-term blood glucose control in diabetes research and management.

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Lab products found in correlation

Edta coated microvette, by Sarstedt (2 mentions) Onetouch ultra2 meter, by LifeScan (1 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (1 mentions) Mouse igf 1, by R&D Systems (1 mentions) Mouse insulin elisa, by Mercodia (1 mentions) Glucose assay kit, by Abcam (1 mentions) Protein carbonyl content assay kit, by Abcam (1 mentions) Lipid peroxidation colorimetric assay kit, by Abcam (1 mentions) Accu chek performa blood glucose meter, by Roche Diabetes Care (1 mentions) Polarstar omega spectrophotometer, by BMG Labtech (1 mentions) Vacutainer, by BD (1 mentions) Onetouch ultra, by LifeScan (1 mentions)

Close spelling variants of this product

Mouse Hemoglobin A1c (HbA1c) Assay Kit Hemoglobin A1c

7 protocols using mouse hemoglobin a1c kit

1

Fasting Glucose and Insulin Measurement

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Ten-hour fasting blood glucose levels were measured in whole blood drawn from the tail vein using the OneTouch Ultra 2 Meter (LifeScan, Inc. New Brunswick NJ). Insulin and HbA1c levels were determined using an Ultra-Sensitive Mouse Insulin ELISA Kit and Mouse Hemoglobin A1c Kit, respectively (Crystal Chem, Inc. Downers Grove, IL).
Hammoudi N., Jeong D., Singh R., Farhat A., Komajda M., Mayoux E., Hajjar R, & Lebeche D. (2017). Empagliflozin Improves Left Ventricular Diastolic Dysfunction in a Genetic Model of Type 2 Diabetes. Cardiovascular drugs and therapy, 31(3), 233-246.
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2

Serum Biomarker Quantification in Mice

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Serum samples were collected by retro-orbital bleed and were stored at −80°C. Total ghrelin levels were measured using a Mouse Ghrelin (total) ELISA kit by Millipore according to manufacturer’s instructions. Serum leptin concentrations were measured by ELISA (Mouse Leptin DuoSet, R&D Systems) as per the manufacturer’s instructions. Insulin concentrations in serum and glucose-stimulated insulin secretion supernatant were measured by ELISA (cat. no. 10-1247-01; mouse insulin ELISA, Mercodia), as per the manufacturer’s instructions. HbA1c was measured using a Mouse Hemoglobin A1c kit for the quantitative determination of HbA1c in mouse whole blood from Crystal Chem per the manufacturer’s instructions. Serum IGF-1 concentrations were measured by ELISA (Mouse IGF-1, R&D Systems) per the manufacturer’s instructions.
Dooley J., Garcia-Perez J.E., Sreenivasan J., Schlenner S.M., Vangoitsenhoven R., Papadopoulou A.S., Tian L., Schonefeldt S., Serneels L., Deroose C., Staats K.A., Van der Schueren B., De Strooper B., McGuinness O.P., Mathieu C, & Liston A. (2015). The microRNA-29 Family Dictates the Balance Between Homeostatic and Pathological Glucose Handling in Diabetes and Obesity. Diabetes, 65(1), 53-61.
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3

Evaluation of Metabolic Biomarkers in Mice

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A portion of the whole blood collected at the sacrifice of all experimental animals was used for hemoglobin A1c (HbA1c) measurement, using a Mouse Hemoglobin A1c Kit (Crystal Chem Inc., USA). The remaining whole blood was centrifuged (3,000 rpm at 4°C for 20 min) to collect serum samples. Glucose Assay Kits, Insulin (Mouse) ELISA Kits, Protein Carbonyl Content Assay Kit (Biovision), and a TNF-α and IL-1β R&D Duoset ELISA kit (R&D Systems, Minneapolis, MN, USA) were used for serum or urine analysis.
The isolated liver was homogenized and lipid peroxidation was measured using the Lipid Peroxidation Colorimetric Assay Kit (Biovision). All experiments were conducted according to the specifications of the manufacturers.
Park S.J., Lee D., Kim D., Lee M., In G., Han S.T., Kim S.W., Lee M.H., Kim O.K, & Lee J. (2019). The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in vivo. Journal of Ginseng Research, 44(2), 362-372.
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4

Characterizing Glucose Metabolism in Mice

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The wt and ob/ob mice were weighed, and peripheral blood was collected for measurements of blood glucose and glycated hemoglobin (HbA1c). Blood was collected in sterile EDTA-treated tubes (#367838, BD Vacutainer; BD, Franklin Lakes, NJ) and stored at 4 °C. Blood glucose levels were measured using an Accu-Chek Performa blood glucose meter (Roche Diabetes Care, Sydney, Australia). HbA1c was measured using a Mouse Hemoglobin A1c Kit (#80310; Crystal Chem, Elk Grove Village, IL) according to the manufacturer’s instructions. HbA1c levels were read on a POLARstar Omega Spectrophotometer (BMG LABTECH, Ortenberg, Germany). Samples were averaged with n=8 per biological group, and statistical significance (p<0.05) was measured using a Student t test.
Natoli R., Fernando N., Dahlenburg T., Jiao H., Aggio-Bruce R., Barnett N.L., Chao de la Barca J.M., Tcherkez G., Reynier P., Fang J., Chu-Tan J.A., Valter K., Provis J, & Rutar M. (2018). Obesity-induced metabolic disturbance drives oxidative stress and complement activation in the retinal environment. Molecular Vision, 24, 201-217.
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5

Glycated Hemoglobin A1c Analysis

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The cardiac blood was collected in an EDTA-coated microvette (Sarstedt AG and Co. Numbrecht, Germany) and frozen at −20˚C. We analyzed the thawed blood for glycated hemoglobin A1c content using the mouse hemoglobin A1c kit (Crystal Chem, Downers Grove, IL).
Toh H., Smolentsev A., Bozadjian R.V., Keeley P.W., Lockwood M.D., Sadjadi R., Clegg D.O., Blodi B.A., Coffey P.J., Reese B.E, & Thomson J.A. (2019). Vascular changes in diabetic retinopathy – A longitudinal study in the Nile rat. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1547-1560.
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6

Glycated Hemoglobin A1c Analysis

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The cardiac blood was collected in an EDTA-coated microvette (Sarstedt AG and Co. Numbrecht, Germany) and frozen at −20˚C. We analyzed the thawed blood for glycated hemoglobin A1c content using the mouse hemoglobin A1c kit (Crystal Chem, Downers Grove, IL).
Toh H., Smolentsev A., Bozadjian R.V., Keeley P.W., Lockwood M.D., Sadjadi R., Clegg D.O., Blodi B.A., Coffey P.J., Reese B.E, & Thomson J.A. (2019). Vascular changes in diabetic retinopathy – A longitudinal study in the Nile rat. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1547-1560.
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7

Glycemic Changes in IAPP Transgenic Mice

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Long term changes (7–54 weeks) in non-fasting blood glucose and glycosylated hemoglobin levels were assessed in wild type, hemizygous (IAPP+/−) and homozygous (IAPP+/+) male and female mice. Blood was collected from mice tail vein, and blood glucose levels were determined using glucometer (ONE TOUCH Ultra, LIFESCAN). Glycated hemoglobin (HbA1c) levels were measured using Mouse Hemoglobin A1C kit (Crystal Chem., IL, cat. No. 80310) following manufacturer’s instruction. Briefly, equal volumes (5µl) of total blood from each group were collected and subjected to extensive proteases digestion, following which glycated hemoglobin levels were determined using horseradish peroxidase based colorimetric assay. The row data (absorbance values at 700nm) were converted to % HbaA1c using manufacturer protocol.
Singh S., Bhowmick D.C., Pany S., Joe M., Zaghlula N, & Jeremic A.M. (2018). Apoptosis Signal Regulating Kinase-1 and NADPH Oxidase Mediate Human Amylin Evoked Redox Stress and Apoptosis in Pancreatic Beta-Cells. Biochimica et biophysica acta. Biomembranes, 1860(9), 1721-1733.
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