The following reagents with catalogue numbers were acquired from the indicated vendors: EHT1864 #E1657 (Sigma-Aldrich, St. Louis, MO); monoclonal mouse anti-Rac1 #ARC-03, polyclonal rabbit anti-Rac #ARC-01, polyclonal rabbit anti-Rho #ARH-02, monoclonal mouse anti-Cdc42 #ACD-03 (Cytoskeleton, Denver, CO); Alexa Fluor 594 Phalloidin #A12381, Alexa Fluor 488 Phalloidin #A12379 (ThermoFisher Scientific, Waltham, MA); monoclonal mouse anti-apCAM clone 4E8 (gift from E. Kandel, Columbia University); CK666 #182515, CK689 #182517, polyclonal rabbit anti-p34 Arc/ARPC2 #07-227, monoclonal mouse anti-cortactin (p80/85, clone 4F11) #05-180 (EMD Millipore, Billerica, MA), rabbit monoclonal anti-WAVE2 (clone D2C8) XP #3659 (Cell Signaling Technology, Danvers, MA).
Anti cdc42 acd 03
Manufactured by Cytoskeleton
Anti-Cdc42 #ACD-03 is a laboratory reagent that binds to and detects the Cdc42 protein. Cdc42 is a small Rho-family GTPase involved in regulating cellular processes such as cytoskeletal organization, cell morphology, and cell cycle progression. This product can be used to study the expression, localization, and activity of Cdc42 in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Eht1864, by Merck Group (1 mentions)
Alexa fluor 594 phalloidin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Anti pan akt clone c67e7, by Cell Signaling Technology (1 mentions)
Anti rhoa arh04, by Cytoskeleton (1 mentions)
Anti rac1, by Cytoskeleton (1 mentions)
Proteoextract native membrane protein extraction kit, by Merck Group (1 mentions)
Anti e cadherin clone 36, by BD (1 mentions)
2 protocols using anti cdc42 acd 03
1
Rho GTPase Signaling Pathway Assay
2
Membrane Protein Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein lysates and conditioned media (CM) samples were prepared as previously described (Kamata et al., 2015 (link), 2020 (link)). Membrane protein purification was performed using a ProteoExtract® Native Membrane Protein Extraction Kit (Merck) according to the manufacturer's instructions. Immunoblotting and enzyme-linked immunosorbent assay (ELISA) were performed as previously described (Kamata et al., 2015 (link), 2020 (link)). Primary antibodies used for immunoblotting were as follows: anti-CCL6 (ab83134, Abcam; 1:2000), anti-P-AKT (clone D9E, Cell Signaling Technology; 1:2000), anti-pan-AKT (clone C67E7, Cell Signaling Technology; 1:5000), anti-RAC1 (ARC03, Cytoskeleton, Inc.; 1:2000), anti-RHOA (ARH04, Cytoskeleton, Inc.; 1:2000), anti-CDC42 (ACD03, Cytoskeleton, Inc.; 1:2000), anti-pan-RAS (clone EP1125Y, Merck; 1:2000), anti-E-cadherin (clone 36, BD Biosciences; 1:1000), anti-GAPDH (clone GA1R, Thermo Fisher Scientific; 1:4000) and anti-MAC2 (CL8942AP, Cedarlane; 1:2000) antibodies. Antibody validation profiles were provided by respective companies upon purchase.
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