Log In Sign up
The largest database of trusted experimental protocols

Cd23 b3b4

Manufactured by Thermo Fisher Scientific
Visit Supplier

The CD23 (B3B4) is a lab equipment product by Thermo Fisher Scientific. It is a cell surface marker that is expressed on B cells and activated monocytes. The CD23 antibody can be used for the identification and enumeration of B cells and activated monocytes in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Igd 11 26c 2a, by BioLegend (2 mentions) Gr 1 rb6 8c5, by BD (2 mentions) Cd8α 53 6, by Thermo Fisher Scientific (2 mentions) Mouse anti myc tag 4a6, by Merck Group (2 mentions) Pax5 1h9, by Thermo Fisher Scientific (2 mentions) Igm il 41, by Thermo Fisher Scientific (2 mentions) Cd4 rm405, by BioLegend (2 mentions) Rabbit anti myc tag 71d10, by Cell Signaling Technology (2 mentions) Tcf1 c63d9, by Cell Signaling Technology (2 mentions) 2 nbdg, by Cayman Chemical (2 mentions) Cd44 im7, by Thermo Fisher Scientific (1 mentions) Nk1.1 pk136, by Thermo Fisher Scientific (1 mentions) Cd8a 53 6, by Thermo Fisher Scientific (1 mentions) Pd 1 rmp1 30, by BioLegend (1 mentions) B220 ra3 6b2, by Thermo Fisher Scientific (1 mentions) Fas 15a7, by Thermo Fisher Scientific (1 mentions) Cd19 1d3, by Thermo Fisher Scientific (1 mentions) Cxcr5 2g8, by BD (1 mentions) Tcrb h57 597, by Thermo Fisher Scientific (1 mentions) Cd4 rm4 5, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD23 (B3B4, (3 citations) CD23 (clone B3B4) (2 citations)

3 protocols using cd23 b3b4

1

Single-cell Immunophenotyping and Glucose Uptake

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were prepared and stained as previously described (Lin et al., 2016 (link)). Antibodies used include rabbit anti-myc tag (71D10, Cell Signaling Technology), mouse anti-myc tag (4A6, Millipore), TCF1 (C63D9, Cell Signaling Technology), Pax5 (1H9, eBioscience), IgM (Il/41, eBioscience), IgD (11-26c.2a, BioLegend), CD23 (B3B4, eBioscience), CD4 (RM405, BioLegend), CD8α (53-6.7, eBioscience), and Gr1 (RB6-8C5, BD PharMingen). Glucose uptake was determined by staining with the fluorescent glucose analog 2-NBDG (Cayman, 100 μM) in glucose-free RPMI.
Chen Y.H., Kratchmarov R., Lin W.H., Rothman N.J., Yen B., Adams W.C., Nish S.A., Rathmell J.C, & Reiner S.L. (2018). Asymmetric PI3K Activity in Lymphocytes Organized by a PI3K-Mediated Polarity Pathway. Cell reports, 22(4), 860-868.
+ Open protocol
+ Expand
2

Single-cell Immunophenotyping and Glucose Uptake

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were prepared and stained as previously described (Lin et al., 2016 (link)). Antibodies used include rabbit anti-myc tag (71D10, Cell Signaling Technology), mouse anti-myc tag (4A6, Millipore), TCF1 (C63D9, Cell Signaling Technology), Pax5 (1H9, eBioscience), IgM (Il/41, eBioscience), IgD (11-26c.2a, BioLegend), CD23 (B3B4, eBioscience), CD4 (RM405, BioLegend), CD8α (53-6.7, eBioscience), and Gr1 (RB6-8C5, BD PharMingen). Glucose uptake was determined by staining with the fluorescent glucose analog 2-NBDG (Cayman, 100 μM) in glucose-free RPMI.
Chen Y.H., Kratchmarov R., Lin W.H., Rothman N.J., Yen B., Adams W.C., Nish S.A., Rathmell J.C, & Reiner S.L. (2018). Asymmetric PI3K Activity in Lymphocytes Organized by a PI3K-Mediated Polarity Pathway. Cell reports, 22(4), 860-868.
+ Open protocol
+ Expand
3

Comprehensive Flow Cytometry for Immune Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry reagents used were: TCRb (H57-597, eBioscience), CD4 (RM4-5, eBioscience), CD8a (53–6.7, eBioscience), CD21 (8D9, eBioscience), CD23 (B3B4, eBioscience), CD44 (IM7, eBioscience), NK1.1 (PK136, eBioscience), CD1d tetramer (PBS57, NIH Tetramer Core Facility), 2B4 (2B4, BD Biosciences), Ly9 (Ly9ab3, Biolegend), B220 (RA3-6B2, eBioscience), CD19 (1D3, eBioscience), Fas (15A7, eBioscience), GL-7 (GL-7, eBioscience), PD-1 (RMP-130, Biolegend), CXCR5 (2G8, BD Biosciences), biotin goat anti-rat (cat# 112-067-003, Jackson Immunoresearch), fluorophore-conjugated SAv (eBioscience). CXCR5 staining was performed as previously described [31 (link)]. Dead cells were excluded by staining with LIVE/DEAD Fixable Aqua Dead Cell Stain (Thermo).
Huang B., Gomez-Rodriguez J., Preite S., Garrett L.J., Harper U.L, & Schwartzberg P.L. (2016). CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development. PLoS ONE, 11(6), e0156072.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!