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Anti hoip

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Anti-HOIP is a recombinant protein produced by R&D Systems. It functions as an antibody that specifically binds to and detects the HOIP protein.

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Lab products found in correlation

Anti flag m2, by Merck Group (3 mentions) Anti ikkα, by Cell Signaling Technology (2 mentions) Anti lexa, by Merck Group (2 mentions) Anti rbck1 hoil 1l, by Merck Group (2 mentions) Anti tradd 3684, by Cell Signaling Technology (2 mentions) Anti ikbkg nemo antibodies, by Merck Group (2 mentions) Anti actin, by Merck Group (2 mentions) U2os htb 96, by American Type Culture Collection (2 mentions) Anti traf2, by Cell Signaling Technology (2 mentions) Anti tnfr1, by Cell Signaling Technology (2 mentions) Anti tak1 4505, by Cell Signaling Technology (2 mentions) Anti gfp, by Takara Bio (2 mentions) Anti myc 9e10 mms 150p, by Fortrea (2 mentions) Anti dgkζ, by Abcam (1 mentions) Anti β catenin, by BD (1 mentions) Anti erk, by Santa Cruz Biotechnology (1 mentions) Anti snx27, by Proteintech (1 mentions) Anti atp7a, by Santa Cruz Biotechnology (1 mentions) Anti normal mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions)

3 protocols using anti hoip

1

Comprehensive Antibody Validation Protocol

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Antibodies used are anti-LexA (06-719, Millipore), anti-Myc 9E10 (MMS-150P, Covance), anti-Flag M2 (A8592, Sigma Aldrich), anti-GFP (632592, Clontech), anti-Actin (A2066, Sigma Aldrich), anti-HOIP (MAB8039, R&D systems), anti-RBCK1/HOIL-1L (HPA024185, Sigma Aldrich). Anti-Phospho-IκBα (2859), anti-IκBα (4814), anti-Phospho-p65 (3033), anti-p65 (8242), anti-TRADD (3684), anti-TRAF2 (4712), anti-IKKα (11930), anti-TNF-R1 (3736) and anti-TAK1 (4505) were purchased from Cell Signaling. Anti-IKBKG/NEMO antibodies were purchased from Sigma Aldrich (SAB1404591) and Millipore (05-631).
Cell lines used in this study HEK293T (CRL-3216), HeLa (CCL-2), CaCo-2 (HTB-37) and U2OS (HTB-96) were purchased from ATCC. Parental cell lines were tested and determined to be mycoplasma free.
de Jong M.F., Liu Z., Chen D, & Alto N.M. (2016). Shigella flexneri suppresses NF-kB activation by inhibiting linear ubiquitin chain ligation. Nature microbiology, 1(7), 16084.
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2

Comprehensive Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used are anti-LexA (06-719, Millipore), anti-Myc 9E10 (MMS-150P, Covance), anti-Flag M2 (A8592, Sigma Aldrich), anti-GFP (632592, Clontech), anti-Actin (A2066, Sigma Aldrich), anti-HOIP (MAB8039, R&D systems), anti-RBCK1/HOIL-1L (HPA024185, Sigma Aldrich). Anti-Phospho-IκBα (2859), anti-IκBα (4814), anti-Phospho-p65 (3033), anti-p65 (8242), anti-TRADD (3684), anti-TRAF2 (4712), anti-IKKα (11930), anti-TNF-R1 (3736) and anti-TAK1 (4505) were purchased from Cell Signaling. Anti-IKBKG/NEMO antibodies were purchased from Sigma Aldrich (SAB1404591) and Millipore (05-631).
Cell lines used in this study HEK293T (CRL-3216), HeLa (CCL-2), CaCo-2 (HTB-37) and U2OS (HTB-96) were purchased from ATCC. Parental cell lines were tested and determined to be mycoplasma free.
de Jong M.F., Liu Z., Chen D, & Alto N.M. (2016). Shigella flexneri suppresses NF-kB activation by inhibiting linear ubiquitin chain ligation. Nature microbiology, 1(7), 16084.
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3

Antibody Characterization for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following antibodies were used at a dilution of 1:1000 except where otherwise stated: Anti-Actin (sc-1616 HRP; 1:10,000), anti-ATP7A (sc-376467; 1:100), anti-normal mouse IgG (sc-2025; 1.4 µg for IP), anti-ERK (sc-514302) from Santa Cruz; anti-GFP (2555), anti-OTULIN (14127; 1.4 µg for IP), anti-IκBα (4814), anti-phospho-IκBα (Ser32/36) (9246), anti-LAMP1 (15665; 1:100), anti-phospho-ERK (Thr202/Tyr204) (9101), anti-normal rabbit IgG (2729; 1.4 µg for IP), anti-EEA1 (3288, 1:100) all CST; anti-SNX27 (ab77799; 1.4 µg for IP and 1:50 for IF), anti-VPS26 (ab23892), anti-VPS35 (ab157220; 1:10,000), anti-MRP4 (ab15602; 1:2500), anti-DGKζ (ab105195; 1:100), anti-GLUT1 (ab15309; 1:100 and ab115730) all Abcam; anti-Beta PIX (07-1450-I; 1:5000), anti-Met1-Ub (MABS199) all Millipore; anti-STEAP3 (17186-1-AP), anti-KIDINS220 (21856-1-AP) all Proteintech; anti-SNX27 (for murine SNX27) gift from W. Hong; anti-Beta-catenin (610153, 1:2000) BD; anti-FLAG M2 (F3165; 1:10000 and 1 µg for IP) Sigma; anti-HOIP (MAB8039; 1:5000) R&D; anti-HA (Core facility monoclonal antibodies HMGU; 25 µl for IP); anti-CD2-APC (17-0029-41; 1:200) eBioscience.
Stangl A., Elliott P.R., Pinto-Fernandez A., Bonham S., Harrison L., Schaub A., Kutzner K., Keusekotten K., Pfluger P.T., El Oualid F., Kessler B.M., Komander D, & Krappmann D. (2019). Regulation of the endosomal SNX27-retromer by OTULIN. Nature Communications, 10, 4320.
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