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Syber green 1 reagents

Manufactured by Thermo Fisher Scientific
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SYBER Green I reagents are a commonly used fluorescent dye used for nucleic acid detection and quantification in various molecular biology applications, such as real-time PCR and gel electrophoresis. The reagents bind to double-stranded DNA, emitting a fluorescent signal that can be detected and measured to determine the presence and quantity of DNA in a sample.

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Lab products found in correlation

Superscript 3 first strand synthesis kit, by Thermo Fisher Scientific (3 mentions) Viia 7 real time pcr system, by Thermo Fisher Scientific (3 mentions) Rneasy plus micro kit, by Qiagen (1 mentions)

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Syber Green reagents

3 protocols using syber green 1 reagents

1

RT-qPCR Expression Analysis Protocol

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cDNA was made using Superscript III First-Strand Synthesis kit (Invitrogen). RT-qPCR was performed in triplicate with SYBER Green I reagents (Invitrogen) using 5.0ng cDNA per reaction on the ViiATM 7 Real-Time PCR system (Invitrogen – Life Technologies). Data were analyzed by ViiATM software, Microsoft Excel and PRISM. Gene-specific primers were designed and are listed in Supplementary Table 1.
Mesa K.R., Rompolas P., Zito G., Myung P., Sun T.Y., Brown S., Gonzalez D., Blagoev K.B., Haberman A.M, & Greco V. (2015). Niche induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature, 522(7554), 94-97.
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2

RT-qPCR Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA was made using Superscript III First-Strand Synthesis kit (Invitrogen). RT-qPCR was performed in triplicate with SYBER Green I reagents (Invitrogen) using 5.0ng cDNA per reaction on the ViiATM 7 Real-Time PCR system (Invitrogen – Life Technologies). Data were analyzed by ViiATM software, Microsoft Excel and PRISM. Gene-specific primers were designed and are listed in Supplementary Table 1.
Mesa K.R., Rompolas P., Zito G., Myung P., Sun T.Y., Brown S., Gonzalez D., Blagoev K.B., Haberman A.M, & Greco V. (2015). Niche induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature, 522(7554), 94-97.
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3

Quantitative Gene Expression Analysis

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Total RNA from FACS-isolated cells was isolated from sorted cells using the RNeasy Plus Micro Kit (Qiagen) and quantified. After quantification RNA was reverse transcribed using Superscript III First-Strand Synthesis kit (Invitrogen). cDNA levels were equalized across samples. Using the ViiA™ 7 Real-Time PCR system (Invitrogen – Life Technologies), qRT-PCR was performed in triplicate with SYBER Green I reagents (Invitrogen) using 4.5–5.0ng cDNA per 10µl reaction. Data were analyzed by ViiA™ software and Microsoft Excel and PRISM. Gene-specific primers were designed and are listed in Table S1.
Deschene E.R., Myung P., Rompolas P., Zito G., Sun T.Y., Taketo M.M., Saotome I, & Greco V. (2014). β-catenin activation regulates tissue growth non-cell autonomously in the hair stem cell niche. Science (New York, N.Y.), 343(6177), 1353-1356.
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