Opteia mouse il 10 elisa set

EL4 cells (a T-cell lymphoma cell line derived from B6) and 2.43 hybridomas (used to produce anti-mouse CD8 antibodies (Ab)) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Phoenix GP and Phoenix Eco cell lines were provided by Garry Nolan (Stanford University, USA). D3116, a mouse mesenchymal stem cell (MSC) clone, was kindly provided by Sun U. Song (Inha University, Incheon, Korea).^{40 (link)} The MSCV retroviral plasmids with IRES-GFP (pMIG) and pMIG containing murine IL-10 cDNA (pMIG-IL-10) were kind gifts from Il-Hoan Oh (The Catholic University, Korea).^{41 (link)} IL-10 retrovirus-producing and the empty vector (EV) retrovirus-producing Phoenix Eco packaging cell lines were generated using pMIG-IL-10 and pMIG according to a previously described method.^{36 (link)} IL-10-transduced and EV-transduced D3116 cells were generated by spin infection with the retroviral supernatants and cell sorting of the GFP-positive population with a FACSAria cell sorter (Becton Dickinson, Franklin Lakes, NJ, USA). IL-10 production from MSCs was quantified by measuring the amount of IL-10 accumulated in the culture supernatant over a 48 h-culture period by ELISA (BD OptEIA mouse IL-10 ELISA Set, BD Biosciences, San Jose, CA, USA).