Ae163

Manufactured by Mettler Toledo

Sourced in United States

The AE163 is a precision analytical balance from Mettler Toledo. It is designed to provide accurate and reliable weighing results for a variety of laboratory applications. The balance features a maximum capacity of 160 grams and a readability of 0.1 milligrams.

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Lab products found in correlation

Me204, by Mettler Toledo (1 mentions) Dma 5000 m densimeter, by Anton Paar (1 mentions)

10 protocols using ae163

Spleen Measurement in Mice

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Following the euthanasia of mice, the spleens were carefully removed. Apart from visual examination, the length of each spleen was determined using a scale in cm and the spleen weight was recorded in g using a digital balance (AE163, Mettler, Columbus, OH, USA).
The measurements are expressed as mean ± SD (n = 3 for experimental group and n = 4 for control). The values for the affected and control mice were then statistically analyzed using one-way ANOVA test followed by Dunnett multiple comparison post hoc analysis.

Jabeen M., Boisgard A.S., Danoy A., El Kholti N., Salvi J.P., Boulieu R., Fromy B., Verrier B, & Lamrayah M. (2020). Advanced Characterization of Imiquimod-Induced Psoriasis-Like Mouse Model. Pharmaceutics, 12(9), 789.

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Extensive Physical Characterization of PLG-OMP and iPLG-OMP

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Both PLG-OMP and iPLG-OMP underwent extensive physical characterization, as described below. pH measurements were performed using pHmetro Orma electronic p200; solution density was evaluated using the hydrostatic method (Mettler AE163, Columbus, OH, USA) calibrated with water for HPLC use; osmolality was measured by a Roebling osmometer (calibrated with NaCl 300 mOsm/kg H₂O), cinematic viscosity was determined by a Cannon-Fenske (50) viscosimeter under ASTM D-445 standard procedure, using water for HPLC use as a reference(Cannon, State College, PA, USA); liquid surface tension was measured using a Force Tensiometer (KRŰSS-K6, KRŰSS, Hamburg, Germany). All measures were performed under controlled temperature (22 ± 1 °C).

Piras A.M., Zambito Y., Lugli M., Ferro B., Roncucci P., Mori F., Salvatore A., Ascione E., Bellini M, & Crea R. (2020). Repurposing of Plasminogen: An Orphan Medicinal Product Suitable for SARS-CoV-2 Inhalable Therapeutics. Pharmaceuticals, 13(12), 425.

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Fruit Water Content Determination

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The water content was calculated by dividing the mass of the oven-dried fruit by the mass of the wet or dry fruit. The determination of the fruit water content was conducted by oven drying 10 g of fruit in duplicates at 105°C (Termaks TS8056, Mettler AE163 / Mettler Toledo ME204).

Blunk S., Hoffer J., Brosda S., de Heer M.I., Sturrock C.J, & Mooney S.J. (2020). Impact of fruit orientation and pelleting material on water uptake and germination performance in artificial substrate for sugar beet. PLoS ONE, 15(5), e0232875.

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Moisture Content Determination in Sponge Cake

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Five replicates with dimension size of 6 × 5 × 2 cm³ were cut and weighed (about 22 g). Then, the water content was determined according to the NF V03-707 standard by weighing (AE 163, Mettler-Toledo, Columbus, OH, USA, precision 10⁻⁴ g) them before and after drying in an oven (Termaks TS4057, Bergen, Norway) set at 105 °C for 90 min. The water content was calculated according to Equation (1):

X_{w} = \frac{m_{0} - m_{f}}{m_{0}} \times 100

where

X_{w}

is the water content of the sponge cake sample,

m_{0}

is the mass (kg) of the wet sample and

m_{f}

is the mass (kg) of the dried sample.

Zennoune A., Latil P., Ndoye F.T., Flin F., Perrin J., Geindreau C, & Benkhelifa H. (2021). 3D Characterization of Sponge Cake as Affected by Freezing Conditions Using Synchrotron X-ray Microtomography at Negative Temperature. Foods, 10(12), 2915.

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Measuring Leaf Characteristics Dynamics

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Leaves were weighed on a four-point balance (AE163; Mettler Toledo Ltd, Leicester, UK) and leaf widths were measured at the widest part. Both weight and width of each leaf were repeatedly recorded for 2h at 15min intervals in a cabinet with constant environment (23~24 °C, RH 60%). Later leaf curling characteristics were recorded and oven-dried leaves were weighed. Owing to the different starting and ending points for different species, all 165 leaf curling models were fitted manually. Leaf width loss rates were slopes of curves of the relative leaf width (% hr^–1) and absolute leaf width (mm hr^–1) against time. Leaf weight loss rate (% hr^–1) was the slope of relative water content against time. During the first hour, leaf stomata were assumed to be still open, but in the next hour most leaves had already curled with stomata closed, and cuticular conductance could therefore be calculated without stomatal transpiration (Boyer et al., 1997 (link)). In this study, leaf cuticular conductance (mmol m^–2 s^–1) was the slope of the weight loss curve normalized by original leaf area during the second hour. Relative width/weight loss (% hr^–1) was also calculated as width/weight ratio against time.

Liu H, & Osborne C.P. (2014). Water relations traits of C4 grasses depend on phylogenetic lineage, photosynthetic pathway, and habitat water availability. Journal of Experimental Botany, 66(3), 761-773.

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Monoolein Sample Preparation Protocol

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The concentration of monoolein samples varied from 0.5–5 wt %, with the most successful samples prepared at < 1 wt%. Monoolein powder (~10 mg) and milliQ water (~1.2 g) were weighed on an analytical balance (Mettler AE 163). The sample was heated in an oven at 60 °C for 10 minutes and sonicated briefly, 3 times 3-sec. bursts, at room temperature. The sample was replaced at 60 °C for 10 minutes, resonicated in short bursts, replaced at 60 °C for 30 minutes and resonicated in short bursts. Care was taken not to cause foam on the top of the sample from the sonication. This sample preparation differed from the usual protocol of temperature cycling between 60 °C and −20 °C with vortexing,^{54 (link)} since vortexing and cold temperature caused the lipid to clump. Samples with a monoolein concentration > 1 wt % also clumped more easily. The sample was then loaded into the DMA 5000M densimeter (Anton-Paar) and held at 50 °C for three days prior to the first cooling scan. Six heating and cooling scans between 7 °C and 50 °C were performed with the density recorded at 0.5 °C increments. The heating scans occurred at a rate of 12 °C/hr and the cooling scans at 4 °C/hr. Data were averaged using the Origin 8 software and the standard deviation was calculated.

Reese C.W., Strango Z.I., Dell Z.R., Tristram-Nagle S, & Harper P.E. (2015). Structural insights into the cubic-hexagonal phase transition kinetics of monoolein modulated by sucrose solutions. Physical chemistry chemical physics : PCCP, 17(14), 9194-9204.

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Density Measurement of Sponge Cake

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After batter preparation, the density was determined by weighing (AE 163, Mettler-Toledo, Columbus, OH, USA, precision 10⁻⁴ g) 100 mL of dough using a measuring cylinder. Two replicates were performed.
After baking, five replicates (5.5 × 5 × 2 cm³) were sampled and weighed (about 20 g), then the apparent density was calculated. In order to determine the density of the model sponge cake without air (real density), the same sample was compacted using a vacuum sealing machine (Foodsaver, V2860, Newell Brands, Atlanta, GA, USA) to remove the air from the pores, and the new dimensions were measured to calculate the new volume.

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Heart Morphometric Measurements in Animals

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Body mass was determined by suspending the carcass from an appropriate spring balance (0.2-, 2.0-, 5.0-, 20-, or 100-kg capacity, Salter, Melbourne). The thoracic cavity was then opened, and the whole heart was removed, sealed in a plastic zipper-lock bag, stored under ice, and transported to the University of Adelaide. In the laboratory, congealed blood was emptied from the chambers, the major blood vessels dissected free, and the heart rinsed clean with an isotonic saline solution (0.90% w/v NaCl). The height, width, and depth of each heart were measured to 0.01 mm with digital callipers and the average calculated. Then each heart was separated into left and right atrium and left and right ventricle. The interatrial septum was dissected as part of the right atrium, and the interventricular septum was dissected as part of the left ventricle, following what have become standard procedures (Fulton et al. 1952; Keen 1955; Joyce et al. 2004 ). Chambers ! 30 g were weighed to 0.1 mg (AE163, Mettler, Greifensee, Switzerland) and chambers 1 30 g were weighed to 1.0 mg (1265 MP, Sartorius, Göttingen, Germany), and then chamber masses were summed to provide total heart mass.

Snelling E.P., Taggart D.A., Maloney S.K., Farrell A.P, & Seymour R.S. (2015). Biphasic Allometry of Cardiac Growth in the Developing Kangaroo Macropus fuliginosus. Physiological and biochemical zoology : PBZ, 88(2).

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Leptin-induced Tissue Sampling in Rats

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After leptin delivery, rats remained anesthetized for one hour on a heat pad. Thereafter, 30 mL cold 0.9% saline were perfused in the circulatory system to remove blood from the brain. The hypothalamus was dissected from the whole brain by a medial incision to the piriform lobes, caudal to the optic chiasm and anterior to the cerebral crus to a depth of 2.5 mm and immediately snap frozen in liquid nitrogen. Several other organs and tissues were removed and weighed (Mettler AE 163). Five fat depots were collected: mesenteric (mWAT), perirenal (pWAT), epididymal for males (eWAT) or parameterial for females (paWAT), retroperitoneal (rtWAT), and interscapular brown adipose tissue (iBAT). Several lean tissues were collected: gastrocnemius, soleus, plantaris, tibialis anterior (TA), and extensor digitorum longus (EDL), heart, liver, and kidneys. The hypothalamus samples were stored at −80 °C until analyses were performed.

Côté I., Green S.M., Toklu H.Z., Morgan D., Carter C.S., Tümer N, & Scarpace P.J. (2017). Differential physiological responses to central leptin overexpression in male and female rats. Journal of neuroendocrinology, 29(12), 10.1111/jne.12552.

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Isolation and Evaluation of Sweet Potato Phenolic Compounds' Bioactivity

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The natural compound 3 (3,5-dicaffeoyl quinic acid), was extracted with ethanol:water (70:30) from lyophilized non-tuberized roots of sweet potato Ipomea batatas and purified by semi-preparative HPLC on a Hibar® 250 mm x 25 mm column filled with Lichrospher® 100 RP18e 5µM stationary phase. The compound was eluted with an acidified water/methanol gradient. 27 Compounds 10a, 10c, 10d, 10e, 4a, 4c, 4d and 12 were administrated pure by weighing after HPLC purification, solubilized at 1 mM in a standard AP3 artificial diet for the pea aphid Acyrthosiphon pisum, 61 and diluted 3 times to obtain the experimental dose range (125 µM-1mM). Stability of compounds was checked by HPLC analysis in AP3 artificial diet samples after feeding bioassays. Toxicity was evaluated by scoring survival daily over the whole nymphal life of the pea aphid (7 days), starting from neonate aphids born on faba bean leaves. Growth was also measured by weighing adult aphids on an analytical microbalance (Mettler AE163) at the closest 10 µg, as fully described previously for testing peptide toxicity. 62 Aphid weights were analysed by ANOVA followed by Tukey-Kramer HSD test for comparing multiple means (treating separately either the dose factor for every compound, or the compound factor, at each dose; see

Li X., Grand L., Pouleriguen T., Queneau Y., da Silva P., Rahbé Y., Poëssel J.L, & Moebs-Sanchez S. (2016). Synthesis of new dicinnamoyl 4-deoxy quinic acid and methyl ester derivatives and evaluation of the toxicity against the pea aphid Acyrthosiphon pisum. Organic & biomolecular chemistry, 14(8).

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