The largest database of trusted experimental protocols

2 protocols using cycloheximide chx n11534

1

Cellular Pathways Investigated Using Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
SD70 (M60194-2s) was purchased from Xcessbio Biosciences Inc; Cycloheximide (CHX; N11534), Calpeptin (C8999), chloroquine (C6628), iodoacetamide (I1149), and N-ethylmaleimide (E-3876) were purchased from Sigma-Aldrich; Sorafenib (8705) was purchased from Cell Signaling Technology; MG-132 (S2619) was purchased from Selleck; Z-VAD-FMK (2163) was purchased from Tocris. Treatment conditions were described in detail in the text.
+ Open protocol
+ Expand
2

Immunoblotting analysis of drug-treated cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drugs used in the study include: Bortezomib (S1013), MLN4924 (S7109), MG132 (S2619), Docetaxel (S1148), Doxorubicin (S1208), Etoposide (S1255), SN‐38 (S4908), and Voreloxin (S7518) from Selleckchem. Cycloheximide (CHX; N11534) was purchased from Sigma‐Aldrich. Drugs were dissolved in dimethyl sulfoxide (DMSO). Prior to drug treatment, cells were plated in six‐well plates. When cells reached 60% confluence, they were treated with indicated drugs for various length of time at the concentration described in corresponding figure legend. After treatment, cells were collected for protein extraction and immunoblotting analysis.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!