Micromass lct premier mass spectrometer

Manufactured by Waters Corporation

The Micromass LCT Premier Mass Spectrometer is a high-performance liquid chromatography-mass spectrometry (LC-MS) system designed for analytical applications. It features a time-of-flight (TOF) mass analyzer that provides accurate mass measurements for the identification and characterization of compounds.

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Lab products found in correlation

Acquity uplc system, by Waters Corporation (3 mentions) 2400 chns analyzer, by PerkinElmer (2 mentions) Emx spectrometer, by Bruker (1 mentions) Nicolet is5 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Cary 50 spectrophotometer, by Agilent Technologies (1 mentions) Accq tag kit, by Waters Corporation (1 mentions)

Close spelling variants of this product

Micromass-LCT Premier Time of Flight (TOF) mass spectrometer Micromass LCT Premier XE Mass Spectrometer LCT premier micromass spectrometer Micromass) LCT® mass spectrometer LCT Premier mass spectrometer Micromass LCT Premier LCT mass spectrometer LCT Premier

7 protocols using micromass lct premier mass spectrometer

Quantifying Methionine Cycle and Glutathione

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Metabolites implicated in the methionine cycle and reduced/oxidized glutathione were determined by LC/MS using a Waters ACQUITY-UPLC system coupled to a Waters Micromass LCT Premier Mass Spectrometer equipped with a Lockspray ionization source as described previously [22] (link).

Fernández-Tussy P., Fernández-Ramos D., Lopitz-Otsoa F., Simón J., Barbier-Torres L., Gomez-Santos B., Nuñez-Garcia M., Azkargorta M., Gutiérrez-de Juan V., Serrano-Macia M., Rodríguez-Agudo R., Iruzubieta P., Anguita J., Castro R.E., Champagne D., Rincón M., Elortza F., Arslanow A., Krawczyk M., Lammert F., Kirchmeyer M., Behrmann I., Crespo J., Lu S.C., Mato J.M., Varela-Rey M., Aspichueta P., Delgado T.C, & Martínez-Chantar M.L. (2019). miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease. Molecular Metabolism, 29, 40-54.

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Characterization of Organometallic Compounds

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Elemental analyses were performed on a Perkin-Elmer 2400 CHNS analyzer. UV-visible (UV-vis) spectra were recorded with a Cary 50 spectrophotometer or an Agilent 8453 spectrophotometer equipped with an Unisoku Unispeks cryostat using either a 0.10 cm or 1.00 cm quartz cuvette. FTIR spectra were collected on a Varian 800 Scimitar Series FTIR spectrometer in air or a Thermo Scientific Nicolet iS5 spectrophotometer with an iD5 an attenuated total reflectance (ATR) attachment in a dinitrogen filled glovebox. High-resolution mass spectra were collected using Waters Micromass LCT Premier Mass Spectrometer. CV experiments were conducted using a CH1600C electrochemical analyzer. A 2.0 mm glassy carbon electrode was used as the working electrode at scan velocities 0.1 V s^–1. The ferrocenium/ferrocene couple [FeCp₂]^+/0 was used as an internal reference to monitor the reference electrode (Ag^0/+). Tetrabutylammonium hexafluorophosphate (TBAP) was use as the supporting electrolyte at a concentration of 0.1 M. Perpendicular-mode X-band EPR spectra were collected using a Bruker EMX spectrometer at 4 K using liquid helium.

Lau N., Sano Y., Ziller J.W, & Borovik A.S. (2018). Modular Bimetallic Complexes with a Sulfonamido-Based Ligand. Dalton transactions (Cambridge, England : 2003), 47(35), 12362-12372.

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Reagent Synthesis and Characterization

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Reagents and solvents were purchased
from Wako Pure Chemical Industries (Osaka, Japan), Sigma-Aldrich (St.
Louis, MO), Watanabe Chemical Industries (Hiroshima, Japan), and Tokyo
Chemical Industries (Tokyo, Japan). All were used as received. Herceptin
was purchased from Chugai Pharmaceutical Co., Ltd. Mass spectra were
obtained on a Waters MICRO MASS LCT-premier mass spectrometer.

Muguruma K., Fujita K., Fukuda A., Kishimoto S., Sakamoto S., Arima R., Ito M., Kawasaki M., Nakano S., Ito S., Shimizu K., Taguchi A., Takayama K., Taniguchi A., Ito Y, & Hayashi Y. (2019). Kinetics-Based Structural Requirements of Human Immunoglobulin G Binding Peptides. ACS Omega, 4(11), 14390-14397.

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Amino Acid Derivatization and UPLC-MS Analysis

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Derivatization of amino acids was achieved using the AccQ-Tag kit obtained from Waters (Millford, MA) as specified in the manufacturer’s instructions. An amino acid standard mixture including 29 amino acids was prepared and diluted into eight concentrations ranging from 20 pmol to 0.1 pmol. An internal standard, norvaline [10 pmol/μl] was added to each sample and the amino-acid standards mixture. The samples were analysed on a Waters Acquity UPLC system coupled to a Micromass LCT Premier mass spectrometer (Waters, Millford, MA) operated in W-mode. The acquired data was exported as NetCDF files. MATLAB 7.11.0 (R2014b) (Mathworks, Natick, MA) was used with an in-house script for alignment and extraction of the integrated peak area for each amino acid.

Mörén L., Perryman R., Crook T., Langer J.K., Oneill K., Syed N, & Antti H. (2018). Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM. BMC Cancer, 18, 167.

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Multi-Technique Characterization of Complexes

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Elemental analyses were performed on a Perkin-Elmer 2400 CHNS analyzer. Electronic absorbance spectra were recorded with a Cary 50 using a 1.00 cm or 0.10 cm quartz cuvette. Fourier transform infrared (FTIR) spectra were collected on a Varian 800 Scimitar Series FTIR spectrometer in air. High-resolution mass spectra were collected using Waters Micromass LCT Premier Mass Spectrometer. Cyclic voltammetry (CV) experiments were conducted using a CH1600C electrochemical analyzer and measured on CH₂Cl₂ solutions of the complexes. A 2.0 mm glassy carbon electrode was used as the working electrode at a scan velocity of 0.1 V s⁻¹ and a Pt wire were used as the auxiliary electrode. A ferrocenium/ferrocene couple ([FeCp₂]^+/0) was used as an internal reference to monitor the reference electrode (Ag^0/+). Tetrabutylammonium hexafluorophosphate (TBAP) was use as the supporting electrolyte at a concentration of 0.1 M.

Sano Y., Lau N., Weitz A.C., Ziller J.W., Hendrich M.P, & Borovik A.S. (2017). Models for Unsymmetrical Active Sites in Metalloproteins: Structural, Redox, and Magnetic Properties of Bimetallic Complexes with MII–(μ-OH)–FeIII Cores. Inorganic chemistry, 56(22), 14118-14128.

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ES-MS Analysis of Compound 1

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ES-MS spectra were recorded with a Waters Micromass LCT Premier mass spectrometer. Sample requirements for this analysis necessitated dissolution of 1 in methanol:water (50:50) with gentle heating.

Al-Mahamad L.L., El-Zubir O., Smith D.G., Horrocks B.R, & Houlton A. (2017). A coordination polymer for the site-specific integration of semiconducting sequences into DNA-based materials. Nature Communications, 8, 720.

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Hepatic SAMe and SAH Quantification

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Hepatic SAMe and SAH were determined by Liquid-chromatography/mass spectrometry (LC/MS) using a Waters ACQUITY-UPLC system coupled to a Waters Micromass LCT Premier Mass Spectrometer equipped with a Lockspray ionization source as described previously^{45 (link)}.

Fernández-Ramos D., Fernández-Tussy P., Lopitz-Otsoa F., Gutiérrez-de-Juan V., Navasa N., Barbier-Torres L., Zubiete-Franco I., Simón J., Fernández A.F., Arbelaiz A., Aransay A.M., Lavín J.L., Beraza N., Perugorria M.J., Banales J.M., Villa E., Fraga M.F., Anguita J., Avila M.A., Berasain C., Iruzibieta P., Crespo J., Lu S.C., Varela-Rey M., Mato J.M., Delgado T.C, & Martínez-Chantar M.L. (2018). MiR-873-5p acts as an epigenetic regulator in early stages of liver fibrosis and cirrhosis. Cell Death & Disease, 9(10), 958.

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